Development and Molecular Cytogenetic Identification of Two Wheat-<i>Aegilops geniculata</i> Roth 7M<sup>g</sup> Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust
Fusarium head blight (<i>Fhb</i>), powdery mildew, and stripe rust are major wheat diseases globally. <i>Aegilops geniculata</i> Roth (U<sup>g</sup>U<sup>g</sup>M<sup>g</sup>M<sup>g</sup>, 2<i>n</i> = 4x = 28), a wil...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/13/7056 |
| _version_ | 1797479813914558464 |
|---|---|
| author | Xiaoying Yang Maoru Xu Yongfu Wang Xiaofang Cheng Chenxi Huang Hong Zhang Tingdong Li Changyou Wang Chunhuan Chen Yajuan Wang Wanquan Ji |
| author_facet | Xiaoying Yang Maoru Xu Yongfu Wang Xiaofang Cheng Chenxi Huang Hong Zhang Tingdong Li Changyou Wang Chunhuan Chen Yajuan Wang Wanquan Ji |
| author_sort | Xiaoying Yang |
| collection | DOAJ |
| description | Fusarium head blight (<i>Fhb</i>), powdery mildew, and stripe rust are major wheat diseases globally. <i>Aegilops geniculata</i> Roth (U<sup>g</sup>U<sup>g</sup>M<sup>g</sup>M<sup>g</sup>, 2<i>n</i> = 4x = 28), a wild relative of common wheat, is valuable germplasm of disease resistance for wheat improvement and breeding. Here, we report the development and characterization of two substitution accessions with high resistance to powdery mildew, stripe rust and <i>Fhb</i> (W623 and W637) derived from hybrid progenies between <i>Ae. geniculata</i> and hexaploid wheat Chinese Spring (CS). Fluorescence in situ hybridization (FISH), Genomic in situ hybridizations (GISH), and sequential FISH-GISH studies indicated that the two substitution lines possess 40 wheat chromosomes and 2 <i>Ae. geniculata</i> chromosomes. Furthermore, compared that the wheat addition line parent W166, the 2 alien chromosomes from W623 and W637 belong to the 7M<sup>g</sup> chromosomes of <i>Ae. geniculata</i> via sequential FISH-GISH and molecular marker analysis. Nullisomic-tetrasomic analysis for homoeologous group-7 of wheat and FISH revealed that the common wheat chromosomes 7A and 7B were replaced in W623 and W637, respectively. Consequently, lines W623, in which wheat chromosomes 7A were replaced by a pair of <i>Ae. geniculata</i> 7M<sup>g</sup> chromosomes, and W637, which chromosomes 7B were substituted by chromosomes 7M<sup>g</sup>, with resistance to <i>Fhb</i>, powdery mildew, and stripe rust. This study has determined that the chromosome 7M<sup>g</sup> from <i>Ae. geniculata</i> exists genes resistant to <i>Fhb</i> and powdery mildew. |
| first_indexed | 2024-03-09T21:51:12Z |
| format | Article |
| id | doaj.art-bb5f6a86306f4cfa812578167d434bc5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:51:12Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bb5f6a86306f4cfa812578167d434bc52023-11-23T20:07:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012313705610.3390/ijms23137056Development and Molecular Cytogenetic Identification of Two Wheat-<i>Aegilops geniculata</i> Roth 7M<sup>g</sup> Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe RustXiaoying Yang0Maoru Xu1Yongfu Wang2Xiaofang Cheng3Chenxi Huang4Hong Zhang5Tingdong Li6Changyou Wang7Chunhuan Chen8Yajuan Wang9Wanquan Ji10College of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaFusarium head blight (<i>Fhb</i>), powdery mildew, and stripe rust are major wheat diseases globally. <i>Aegilops geniculata</i> Roth (U<sup>g</sup>U<sup>g</sup>M<sup>g</sup>M<sup>g</sup>, 2<i>n</i> = 4x = 28), a wild relative of common wheat, is valuable germplasm of disease resistance for wheat improvement and breeding. Here, we report the development and characterization of two substitution accessions with high resistance to powdery mildew, stripe rust and <i>Fhb</i> (W623 and W637) derived from hybrid progenies between <i>Ae. geniculata</i> and hexaploid wheat Chinese Spring (CS). Fluorescence in situ hybridization (FISH), Genomic in situ hybridizations (GISH), and sequential FISH-GISH studies indicated that the two substitution lines possess 40 wheat chromosomes and 2 <i>Ae. geniculata</i> chromosomes. Furthermore, compared that the wheat addition line parent W166, the 2 alien chromosomes from W623 and W637 belong to the 7M<sup>g</sup> chromosomes of <i>Ae. geniculata</i> via sequential FISH-GISH and molecular marker analysis. Nullisomic-tetrasomic analysis for homoeologous group-7 of wheat and FISH revealed that the common wheat chromosomes 7A and 7B were replaced in W623 and W637, respectively. Consequently, lines W623, in which wheat chromosomes 7A were replaced by a pair of <i>Ae. geniculata</i> 7M<sup>g</sup> chromosomes, and W637, which chromosomes 7B were substituted by chromosomes 7M<sup>g</sup>, with resistance to <i>Fhb</i>, powdery mildew, and stripe rust. This study has determined that the chromosome 7M<sup>g</sup> from <i>Ae. geniculata</i> exists genes resistant to <i>Fhb</i> and powdery mildew.https://www.mdpi.com/1422-0067/23/13/7056<i>Aegilops geniculata</i> Rothsubstitution linesFusarium head blight (<i>Fhb</i>)powdery mildewstripe rust |
| spellingShingle | Xiaoying Yang Maoru Xu Yongfu Wang Xiaofang Cheng Chenxi Huang Hong Zhang Tingdong Li Changyou Wang Chunhuan Chen Yajuan Wang Wanquan Ji Development and Molecular Cytogenetic Identification of Two Wheat-<i>Aegilops geniculata</i> Roth 7M<sup>g</sup> Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust International Journal of Molecular Sciences <i>Aegilops geniculata</i> Roth substitution lines Fusarium head blight (<i>Fhb</i>) powdery mildew stripe rust |
| title | Development and Molecular Cytogenetic Identification of Two Wheat-<i>Aegilops geniculata</i> Roth 7M<sup>g</sup> Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust |
| title_full | Development and Molecular Cytogenetic Identification of Two Wheat-<i>Aegilops geniculata</i> Roth 7M<sup>g</sup> Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust |
| title_fullStr | Development and Molecular Cytogenetic Identification of Two Wheat-<i>Aegilops geniculata</i> Roth 7M<sup>g</sup> Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust |
| title_full_unstemmed | Development and Molecular Cytogenetic Identification of Two Wheat-<i>Aegilops geniculata</i> Roth 7M<sup>g</sup> Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust |
| title_short | Development and Molecular Cytogenetic Identification of Two Wheat-<i>Aegilops geniculata</i> Roth 7M<sup>g</sup> Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust |
| title_sort | development and molecular cytogenetic identification of two wheat i aegilops geniculata i roth 7m sup g sup chromosome substitution lines with resistance to fusarium head blight powdery mildew and stripe rust |
| topic | <i>Aegilops geniculata</i> Roth substitution lines Fusarium head blight (<i>Fhb</i>) powdery mildew stripe rust |
| url | https://www.mdpi.com/1422-0067/23/13/7056 |
| work_keys_str_mv | AT xiaoyingyang developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT maoruxu developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT yongfuwang developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT xiaofangcheng developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT chenxihuang developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT hongzhang developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT tingdongli developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT changyouwang developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT chunhuanchen developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT yajuanwang developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust AT wanquanji developmentandmolecularcytogeneticidentificationoftwowheatiaegilopsgeniculatairoth7msupgsupchromosomesubstitutionlineswithresistancetofusariumheadblightpowderymildewandstriperust |