Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives
Bradysia odoriphaga (Diptera: Sciaridae) is a serious pest of Chinese chives cultivated in China. Chemosensory proteins (CSPs) are important components of insect olfactory systems that capture and bind environmental semiochemicals which are then transported to olfactory receptors. Despite their impo...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.989601/full |
| _version_ | 1811203990619160576 |
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| author | Yuting Yang Dengke Hua Jiaqi Zhu Fu Wang Youjun Zhang Youjun Zhang |
| author_facet | Yuting Yang Dengke Hua Jiaqi Zhu Fu Wang Youjun Zhang Youjun Zhang |
| author_sort | Yuting Yang |
| collection | DOAJ |
| description | Bradysia odoriphaga (Diptera: Sciaridae) is a serious pest of Chinese chives cultivated in China. Chemosensory proteins (CSPs) are important components of insect olfactory systems that capture and bind environmental semiochemicals which are then transported to olfactory receptors. Despite their importance, the mechanism of olfaction and related behavioral processes in B. odoriphaga have not been characterized. Here, we found that BodoCSP4 has an important olfactory function. RT-qPCR indicated that BodoCSP4 expression was highest in the heads (antennae removed) of adult males, followed by the antennae of adult males. Competitive binding assays with 33 ligands indicated that BodoCSP4 binds well with methyl allyl disulfide, diallyl disulfide, and n-heptadecane; the corresponding dissolution constants (Ki) were as high as 5.71, 5.71, and 6.85 μM, respectively. 3D-structural and molecular docking indicated that BodoCSP4 has five α-helices and surrounds the ligand with certain hydrophobic residues including Leu60, Leu63, Leu64, Ala67, Val28, Ile30, Ile33, Leu34, and Val86, suggesting these residues help BodoCSP4 bind to ligands. Silencing of BodoCSP4 significantly decreased the attraction of B. odoriphaga males to diallyl disulfide and n-heptadecane but not to methyl allyl disulfide in Y-tube olfaction assays. These results increase our understanding of how BodoCSP4 contributes to host and female localization by B. odoriphaga males. |
| first_indexed | 2024-04-12T03:05:17Z |
| format | Article |
| id | doaj.art-bb61235e8c824ae0bf4702707a76fa86 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-12T03:05:17Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-bb61235e8c824ae0bf4702707a76fa862022-12-22T03:50:32ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-09-011310.3389/fphys.2022.989601989601Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chivesYuting Yang0Dengke Hua1Jiaqi Zhu2Fu Wang3Youjun Zhang4Youjun Zhang5Hubei Engineering Technology Center for Pest Forewarning and Management, Institute of Insect Sciences, Yangtze University, Jingzhou, Hubei, ChinaInstitute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan, Hubei, ChinaHubei Engineering Technology Center for Pest Forewarning and Management, Institute of Insect Sciences, Yangtze University, Jingzhou, Hubei, ChinaHubei Engineering Technology Center for Pest Forewarning and Management, Institute of Insect Sciences, Yangtze University, Jingzhou, Hubei, ChinaHubei Engineering Technology Center for Pest Forewarning and Management, Institute of Insect Sciences, Yangtze University, Jingzhou, Hubei, ChinaDepartment of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaBradysia odoriphaga (Diptera: Sciaridae) is a serious pest of Chinese chives cultivated in China. Chemosensory proteins (CSPs) are important components of insect olfactory systems that capture and bind environmental semiochemicals which are then transported to olfactory receptors. Despite their importance, the mechanism of olfaction and related behavioral processes in B. odoriphaga have not been characterized. Here, we found that BodoCSP4 has an important olfactory function. RT-qPCR indicated that BodoCSP4 expression was highest in the heads (antennae removed) of adult males, followed by the antennae of adult males. Competitive binding assays with 33 ligands indicated that BodoCSP4 binds well with methyl allyl disulfide, diallyl disulfide, and n-heptadecane; the corresponding dissolution constants (Ki) were as high as 5.71, 5.71, and 6.85 μM, respectively. 3D-structural and molecular docking indicated that BodoCSP4 has five α-helices and surrounds the ligand with certain hydrophobic residues including Leu60, Leu63, Leu64, Ala67, Val28, Ile30, Ile33, Leu34, and Val86, suggesting these residues help BodoCSP4 bind to ligands. Silencing of BodoCSP4 significantly decreased the attraction of B. odoriphaga males to diallyl disulfide and n-heptadecane but not to methyl allyl disulfide in Y-tube olfaction assays. These results increase our understanding of how BodoCSP4 contributes to host and female localization by B. odoriphaga males.https://www.frontiersin.org/articles/10.3389/fphys.2022.989601/fullBradysia odoriphagachemosensory protein 4competitive binding assaysRNAiY-tube olfaction assay |
| spellingShingle | Yuting Yang Dengke Hua Jiaqi Zhu Fu Wang Youjun Zhang Youjun Zhang Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives Frontiers in Physiology Bradysia odoriphaga chemosensory protein 4 competitive binding assays RNAi Y-tube olfaction assay |
| title | Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives |
| title_full | Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives |
| title_fullStr | Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives |
| title_full_unstemmed | Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives |
| title_short | Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives |
| title_sort | chemosensory protein 4 is required for bradysia odoriphaga to be olfactory attracted to sulfur compounds released from chinese chives |
| topic | Bradysia odoriphaga chemosensory protein 4 competitive binding assays RNAi Y-tube olfaction assay |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.989601/full |
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