Pathogenesis of plant-associated Pseudomonas aeruginosa in Caenorhabditis elegans model
Abstract Background Pseudomonas aeruginosa is a globally dreaded pathogen that triggers fatality in immuno-compromised individuals. The agricultural ecosystem is a massive reservoir of this bacterium, and several studies have recommended P. aeruginosa to promote plant growth. However, there were lim...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-022-02682-z |
| _version_ | 1797988513794228224 |
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| author | Sakthivel Ambreetha Dananjeyan Balachandar |
| author_facet | Sakthivel Ambreetha Dananjeyan Balachandar |
| author_sort | Sakthivel Ambreetha |
| collection | DOAJ |
| description | Abstract Background Pseudomonas aeruginosa is a globally dreaded pathogen that triggers fatality in immuno-compromised individuals. The agricultural ecosystem is a massive reservoir of this bacterium, and several studies have recommended P. aeruginosa to promote plant growth. However, there were limited attempts to evaluate the health risks associated with plant-associated P. aeruginosa. The current study hypothesized that agricultural P. aeruginosa strains exhibit eukaryotic pathogenicity despite their plant-beneficial traits. Results We have demonstrated that feeding with the plant-associated P. aeruginosa strains significantly affects Caenorhabditis elegans health. Out of the 18 P. aeruginosa strain tested, PPA03, PPA08, PPA10, PPA13, PPA14, PPA17, and PPA18 isolated from cucumber, tomato, eggplant, and chili exhibited higher virulence and pathogenicity. Correlation studies indicated that nearly 40% of mortality in C. elegans was triggered by the P. aeruginosa strains with high levels of pyocyanin (> 9 µg/ml) and biofilm to planktonic ratio (> 8). Conclusion This study demonstrated that plant-associated P. aeruginosa could be a potential threat to human health similar to the clinical strains. Pyocyanin could be a potential biomarker to screen the pathogenic P. aeruginosa strains in the agricultural ecosystem. |
| first_indexed | 2024-04-11T08:05:06Z |
| format | Article |
| id | doaj.art-bb61840ecd3d42bd8a4eeed952a91331 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-11T08:05:06Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-bb61840ecd3d42bd8a4eeed952a913312022-12-22T04:35:36ZengBMCBMC Microbiology1471-21802022-11-0122111010.1186/s12866-022-02682-zPathogenesis of plant-associated Pseudomonas aeruginosa in Caenorhabditis elegans modelSakthivel Ambreetha0Dananjeyan Balachandar1Department of Agricultural Microbiology, Tamil Nadu Agricultural UniversityDepartment of Agricultural Microbiology, Tamil Nadu Agricultural UniversityAbstract Background Pseudomonas aeruginosa is a globally dreaded pathogen that triggers fatality in immuno-compromised individuals. The agricultural ecosystem is a massive reservoir of this bacterium, and several studies have recommended P. aeruginosa to promote plant growth. However, there were limited attempts to evaluate the health risks associated with plant-associated P. aeruginosa. The current study hypothesized that agricultural P. aeruginosa strains exhibit eukaryotic pathogenicity despite their plant-beneficial traits. Results We have demonstrated that feeding with the plant-associated P. aeruginosa strains significantly affects Caenorhabditis elegans health. Out of the 18 P. aeruginosa strain tested, PPA03, PPA08, PPA10, PPA13, PPA14, PPA17, and PPA18 isolated from cucumber, tomato, eggplant, and chili exhibited higher virulence and pathogenicity. Correlation studies indicated that nearly 40% of mortality in C. elegans was triggered by the P. aeruginosa strains with high levels of pyocyanin (> 9 µg/ml) and biofilm to planktonic ratio (> 8). Conclusion This study demonstrated that plant-associated P. aeruginosa could be a potential threat to human health similar to the clinical strains. Pyocyanin could be a potential biomarker to screen the pathogenic P. aeruginosa strains in the agricultural ecosystem.https://doi.org/10.1186/s12866-022-02682-zCaenorhabditis elegansPlant-associated Pseudomonas aeruginosaPathogenicityPyocyaninVirulence factors |
| spellingShingle | Sakthivel Ambreetha Dananjeyan Balachandar Pathogenesis of plant-associated Pseudomonas aeruginosa in Caenorhabditis elegans model BMC Microbiology Caenorhabditis elegans Plant-associated Pseudomonas aeruginosa Pathogenicity Pyocyanin Virulence factors |
| title | Pathogenesis of plant-associated Pseudomonas aeruginosa in Caenorhabditis elegans model |
| title_full | Pathogenesis of plant-associated Pseudomonas aeruginosa in Caenorhabditis elegans model |
| title_fullStr | Pathogenesis of plant-associated Pseudomonas aeruginosa in Caenorhabditis elegans model |
| title_full_unstemmed | Pathogenesis of plant-associated Pseudomonas aeruginosa in Caenorhabditis elegans model |
| title_short | Pathogenesis of plant-associated Pseudomonas aeruginosa in Caenorhabditis elegans model |
| title_sort | pathogenesis of plant associated pseudomonas aeruginosa in caenorhabditis elegans model |
| topic | Caenorhabditis elegans Plant-associated Pseudomonas aeruginosa Pathogenicity Pyocyanin Virulence factors |
| url | https://doi.org/10.1186/s12866-022-02682-z |
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