Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol
Summary The use of the methylotrophic yeast Pichia pastoris (Komagataella phaffi) to produce heterologous proteins has been largely reported. However, investigations addressing the potential of this yeast to produce bulk chemicals are still scarce. In this study, we have studied the use of P. pastor...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-07-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13833 |
| _version_ | 1818456239389016064 |
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| author | Albert Fina Gabriela Coelho Brêda Míriam Pérez‐Trujillo Denise Maria Guimarães Freire Rodrigo Volcan Almeida Joan Albiol Pau Ferrer |
| author_facet | Albert Fina Gabriela Coelho Brêda Míriam Pérez‐Trujillo Denise Maria Guimarães Freire Rodrigo Volcan Almeida Joan Albiol Pau Ferrer |
| author_sort | Albert Fina |
| collection | DOAJ |
| description | Summary The use of the methylotrophic yeast Pichia pastoris (Komagataella phaffi) to produce heterologous proteins has been largely reported. However, investigations addressing the potential of this yeast to produce bulk chemicals are still scarce. In this study, we have studied the use of P. pastoris as a cell factory to produce the commodity chemical 3‐hydroxypropionic acid (3‐HP) from glycerol. 3‐HP is a chemical platform which can be converted into acrylic acid and to other alternatives to petroleum‐based products. To this end, the mcr gene from Chloroflexus aurantiacus was introduced into P. pastoris. This single modification allowed the production of 3‐HP from glycerol through the malonyl‐CoA pathway. Further enzyme and metabolic engineering modifications aimed at increasing cofactor and metabolic precursors availability allowed a 14‐fold increase in the production of 3‐HP compared to the initial strain. The best strain (PpHP6) was tested in a fed‐batch culture, achieving a final concentration of 3‐HP of 24.75 g l−1, a product yield of 0.13 g g−1 and a volumetric productivity of 0.54 g l−1 h−1, which, to our knowledge, is the highest volumetric productivity reported in yeast. These results benchmark P. pastoris as a promising platform to produce bulk chemicals for the revalorization of crude glycerol and, in particular, to produce 3‐HP. |
| first_indexed | 2024-12-14T22:23:30Z |
| format | Article |
| id | doaj.art-bb61c9effc374fd7b88dcb451ef88e06 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-14T22:23:30Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-bb61c9effc374fd7b88dcb451ef88e062022-12-21T22:45:26ZengWileyMicrobial Biotechnology1751-79152021-07-011441671168210.1111/1751-7915.13833Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerolAlbert Fina0Gabriela Coelho Brêda1Míriam Pérez‐Trujillo2Denise Maria Guimarães Freire3Rodrigo Volcan Almeida4Joan Albiol5Pau Ferrer6Department of Chemical, Biological and Environmental Engineering Universitat Autònoma de Barcelona Bellaterra Catalonia SpainDepartamento de Bioquímica, Instituto de Química Universidade Federal do Rio de Janeiro Rio de Janeiro BrazilServei de Ressonància Magnètica Nuclear, Facultat de Ciències i Biociències Universitat Autònoma de Barcelona Bellaterra Catalonia SpainDepartamento de Bioquímica, Instituto de Química Universidade Federal do Rio de Janeiro Rio de Janeiro BrazilDepartamento de Bioquímica, Instituto de Química Universidade Federal do Rio de Janeiro Rio de Janeiro BrazilDepartment of Chemical, Biological and Environmental Engineering Universitat Autònoma de Barcelona Bellaterra Catalonia SpainDepartment of Chemical, Biological and Environmental Engineering Universitat Autònoma de Barcelona Bellaterra Catalonia SpainSummary The use of the methylotrophic yeast Pichia pastoris (Komagataella phaffi) to produce heterologous proteins has been largely reported. However, investigations addressing the potential of this yeast to produce bulk chemicals are still scarce. In this study, we have studied the use of P. pastoris as a cell factory to produce the commodity chemical 3‐hydroxypropionic acid (3‐HP) from glycerol. 3‐HP is a chemical platform which can be converted into acrylic acid and to other alternatives to petroleum‐based products. To this end, the mcr gene from Chloroflexus aurantiacus was introduced into P. pastoris. This single modification allowed the production of 3‐HP from glycerol through the malonyl‐CoA pathway. Further enzyme and metabolic engineering modifications aimed at increasing cofactor and metabolic precursors availability allowed a 14‐fold increase in the production of 3‐HP compared to the initial strain. The best strain (PpHP6) was tested in a fed‐batch culture, achieving a final concentration of 3‐HP of 24.75 g l−1, a product yield of 0.13 g g−1 and a volumetric productivity of 0.54 g l−1 h−1, which, to our knowledge, is the highest volumetric productivity reported in yeast. These results benchmark P. pastoris as a promising platform to produce bulk chemicals for the revalorization of crude glycerol and, in particular, to produce 3‐HP.https://doi.org/10.1111/1751-7915.13833 |
| spellingShingle | Albert Fina Gabriela Coelho Brêda Míriam Pérez‐Trujillo Denise Maria Guimarães Freire Rodrigo Volcan Almeida Joan Albiol Pau Ferrer Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol Microbial Biotechnology |
| title | Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol |
| title_full | Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol |
| title_fullStr | Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol |
| title_full_unstemmed | Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol |
| title_short | Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol |
| title_sort | benchmarking recombinant pichia pastoris for 3 hydroxypropionic acid production from glycerol |
| url | https://doi.org/10.1111/1751-7915.13833 |
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