Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures
IntroductionThe successful cryopreservation of common carp sperm is crucial for its application in aquaculture and selective breeding programs. This study investigates the efficacy of cryopreserving sperm in large containers (5 mL) with a low dilution rate (1:1) in three different cryoprotective med...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-01-01
|
| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2024.1342483/full |
| _version_ | 1827374131410108416 |
|---|---|
| author | Anatolii Sotnikov Marek Rodina David Gela Sergii Boryshpolets Vitaliy Kholodnyy Vojtěch Kašpar Otomar Linhart Borys Dzyuba |
| author_facet | Anatolii Sotnikov Marek Rodina David Gela Sergii Boryshpolets Vitaliy Kholodnyy Vojtěch Kašpar Otomar Linhart Borys Dzyuba |
| author_sort | Anatolii Sotnikov |
| collection | DOAJ |
| description | IntroductionThe successful cryopreservation of common carp sperm is crucial for its application in aquaculture and selective breeding programs. This study investigates the efficacy of cryopreserving sperm in large containers (5 mL) with a low dilution rate (1:1) in three different cryoprotective media and thawing in different conditions.MethodsThe developed method utilizes a low-ionic (hypotonic) cryoprotective medium, freezing with a controlled cooling rate, and high-temperature sperm thawing (60°C). The investigation employs a detailed spermatozoon motility assessment.ResultsPost-thaw motility of 32.3% ± 14% and initial curvilinear velocity of 89 ± 20 μm/s across 30 males were observed. Principal component analysis of sperm kinematic characteristics revealed distinct populations of sperm cells exhibiting varying responses to cryopreservation. The developed method achieved successful fertilization comparable to that of the non-frozen control group using sperm from a single cryotube (2.5 mL, approximately 50 * 109 spermatozoa) to fertilize 200 g of eggs (1:120,000 egg:spz).DiscussionThis novel approach demonstrates an effective cryopreservation protocol for common carp sperm in large-volume cryo-containers in combination with low-ionic cryomedia and high thawing temperature, providing methods well-suited for fisheries practices and selective breeding programs. Future studies of the biological properties of different sperm subpopulations in post-thaw sperm samples can contribute to a deeper understanding of sperm biology, improve cryopreservation techniques, and enhance the success rates of assisted reproductive technologies. |
| first_indexed | 2024-03-08T11:28:17Z |
| format | Article |
| id | doaj.art-bb627dccd7894ae88e0d60aa296c0b2d |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-08T11:28:17Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-bb627dccd7894ae88e0d60aa296c0b2d2024-01-26T04:41:10ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452024-01-011110.3389/fmars.2024.13424831342483Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperaturesAnatolii SotnikovMarek RodinaDavid GelaSergii BoryshpoletsVitaliy KholodnyyVojtěch KašparOtomar LinhartBorys DzyubaIntroductionThe successful cryopreservation of common carp sperm is crucial for its application in aquaculture and selective breeding programs. This study investigates the efficacy of cryopreserving sperm in large containers (5 mL) with a low dilution rate (1:1) in three different cryoprotective media and thawing in different conditions.MethodsThe developed method utilizes a low-ionic (hypotonic) cryoprotective medium, freezing with a controlled cooling rate, and high-temperature sperm thawing (60°C). The investigation employs a detailed spermatozoon motility assessment.ResultsPost-thaw motility of 32.3% ± 14% and initial curvilinear velocity of 89 ± 20 μm/s across 30 males were observed. Principal component analysis of sperm kinematic characteristics revealed distinct populations of sperm cells exhibiting varying responses to cryopreservation. The developed method achieved successful fertilization comparable to that of the non-frozen control group using sperm from a single cryotube (2.5 mL, approximately 50 * 109 spermatozoa) to fertilize 200 g of eggs (1:120,000 egg:spz).DiscussionThis novel approach demonstrates an effective cryopreservation protocol for common carp sperm in large-volume cryo-containers in combination with low-ionic cryomedia and high thawing temperature, providing methods well-suited for fisheries practices and selective breeding programs. Future studies of the biological properties of different sperm subpopulations in post-thaw sperm samples can contribute to a deeper understanding of sperm biology, improve cryopreservation techniques, and enhance the success rates of assisted reproductive technologies.https://www.frontiersin.org/articles/10.3389/fmars.2024.1342483/fullsperm motilitycomputer-assisted sperm analysiscryopreservationCypriniformesartificial reproduction |
| spellingShingle | Anatolii Sotnikov Marek Rodina David Gela Sergii Boryshpolets Vitaliy Kholodnyy Vojtěch Kašpar Otomar Linhart Borys Dzyuba Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures Frontiers in Marine Science sperm motility computer-assisted sperm analysis cryopreservation Cypriniformes artificial reproduction |
| title | Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures |
| title_full | Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures |
| title_fullStr | Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures |
| title_full_unstemmed | Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures |
| title_short | Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures |
| title_sort | optimizing aquaculture scale common carp artificial reproduction a novel approach to sperm cryopreservation using large volume containers and elevated thawing temperatures |
| topic | sperm motility computer-assisted sperm analysis cryopreservation Cypriniformes artificial reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2024.1342483/full |
| work_keys_str_mv | AT anatoliisotnikov optimizingaquaculturescalecommoncarpartificialreproductionanovelapproachtospermcryopreservationusinglargevolumecontainersandelevatedthawingtemperatures AT marekrodina optimizingaquaculturescalecommoncarpartificialreproductionanovelapproachtospermcryopreservationusinglargevolumecontainersandelevatedthawingtemperatures AT davidgela optimizingaquaculturescalecommoncarpartificialreproductionanovelapproachtospermcryopreservationusinglargevolumecontainersandelevatedthawingtemperatures AT sergiiboryshpolets optimizingaquaculturescalecommoncarpartificialreproductionanovelapproachtospermcryopreservationusinglargevolumecontainersandelevatedthawingtemperatures AT vitaliykholodnyy optimizingaquaculturescalecommoncarpartificialreproductionanovelapproachtospermcryopreservationusinglargevolumecontainersandelevatedthawingtemperatures AT vojtechkaspar optimizingaquaculturescalecommoncarpartificialreproductionanovelapproachtospermcryopreservationusinglargevolumecontainersandelevatedthawingtemperatures AT otomarlinhart optimizingaquaculturescalecommoncarpartificialreproductionanovelapproachtospermcryopreservationusinglargevolumecontainersandelevatedthawingtemperatures AT borysdzyuba optimizingaquaculturescalecommoncarpartificialreproductionanovelapproachtospermcryopreservationusinglargevolumecontainersandelevatedthawingtemperatures |