Multi-Omics Analysis of GNL3L Expression, Prognosis, and Immune Value in Pan-Cancer
Guanine nucleotide-binding protein-like 3-like protein (GNL3L) is a novel, evolutionarily conserved, GTP-binding nucleolar protein. This study aimed to investigate the expression, prognosis, and immune value of GNL3L in pan-cancer from multiple omics analyses. Firstly, the expression and prognostic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/19/4595 |
| _version_ | 1797480373639184384 |
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| author | Pei Liu Wenjia Guo Ying Su Chen Chen Yuhua Ma Ping Ma Cheng Chen Xiaoyi Lv |
| author_facet | Pei Liu Wenjia Guo Ying Su Chen Chen Yuhua Ma Ping Ma Cheng Chen Xiaoyi Lv |
| author_sort | Pei Liu |
| collection | DOAJ |
| description | Guanine nucleotide-binding protein-like 3-like protein (GNL3L) is a novel, evolutionarily conserved, GTP-binding nucleolar protein. This study aimed to investigate the expression, prognosis, and immune value of GNL3L in pan-cancer from multiple omics analyses. Firstly, the expression and prognostic value of GNL3L in pan-cancer were discussed using the TIMER2 database, the GEPIA database, the cBioportal database, COX regression analysis, and enrichment analysis. The association of GNL3L with tumor mutational burden (TMB), tumor microsatellite instability (MSI), mismatch repair (MMR) genes, and immune cells was then analyzed. Finally, an esophageal cancer (ESCA) prediction model was established, and GNL3L clone formation assays were performed. The final results showed that GNL3L is differentially expressed in the vast majority of cancers, is associated with the prognosis of various cancers, and may affect cancer occurrence through processes such as ribonucleoprotein, ribosomal RNA processing, and cell proliferation. At the same time, it was found that the correlation between GNL3L and TMB, MSI, MMR, and various immune cells is significant. The established ESCA prediction model had a strong predictive ability, and GNL3L could significantly affect the proliferation of esophageal cancer cells. In conclusion, GNL3L may serve as an important prognostic biomarker and play an immunomodulatory role in tumors. |
| first_indexed | 2024-03-09T21:58:56Z |
| format | Article |
| id | doaj.art-bb668effaf364217b8678124115ecbe9 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T21:58:56Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-bb668effaf364217b8678124115ecbe92023-11-23T19:53:26ZengMDPI AGCancers2072-66942022-09-011419459510.3390/cancers14194595Multi-Omics Analysis of GNL3L Expression, Prognosis, and Immune Value in Pan-CancerPei Liu0Wenjia Guo1Ying Su2Chen Chen3Yuhua Ma4Ping Ma5Cheng Chen6Xiaoyi Lv7College of Information Science, Engineering Xinjiang University, Urumqi 830046, ChinaXinjiang Medical University Affiliated Tumor Hospital, Urumqi 830011, ChinaCollege of Software, Xinjiang University, Urumqi 830046, ChinaCollege of Information Science, Engineering Xinjiang University, Urumqi 830046, ChinaKaramay Central Hospital, Karamay 834099, ChinaCollege of Software, Xinjiang University, Urumqi 830046, ChinaCollege of Software, Xinjiang University, Urumqi 830046, ChinaCollege of Software, Xinjiang University, Urumqi 830046, ChinaGuanine nucleotide-binding protein-like 3-like protein (GNL3L) is a novel, evolutionarily conserved, GTP-binding nucleolar protein. This study aimed to investigate the expression, prognosis, and immune value of GNL3L in pan-cancer from multiple omics analyses. Firstly, the expression and prognostic value of GNL3L in pan-cancer were discussed using the TIMER2 database, the GEPIA database, the cBioportal database, COX regression analysis, and enrichment analysis. The association of GNL3L with tumor mutational burden (TMB), tumor microsatellite instability (MSI), mismatch repair (MMR) genes, and immune cells was then analyzed. Finally, an esophageal cancer (ESCA) prediction model was established, and GNL3L clone formation assays were performed. The final results showed that GNL3L is differentially expressed in the vast majority of cancers, is associated with the prognosis of various cancers, and may affect cancer occurrence through processes such as ribonucleoprotein, ribosomal RNA processing, and cell proliferation. At the same time, it was found that the correlation between GNL3L and TMB, MSI, MMR, and various immune cells is significant. The established ESCA prediction model had a strong predictive ability, and GNL3L could significantly affect the proliferation of esophageal cancer cells. In conclusion, GNL3L may serve as an important prognostic biomarker and play an immunomodulatory role in tumors.https://www.mdpi.com/2072-6694/14/19/4595GNL3Lpan-cancermulti-omicsprognostic analysisimmune |
| spellingShingle | Pei Liu Wenjia Guo Ying Su Chen Chen Yuhua Ma Ping Ma Cheng Chen Xiaoyi Lv Multi-Omics Analysis of GNL3L Expression, Prognosis, and Immune Value in Pan-Cancer Cancers GNL3L pan-cancer multi-omics prognostic analysis immune |
| title | Multi-Omics Analysis of GNL3L Expression, Prognosis, and Immune Value in Pan-Cancer |
| title_full | Multi-Omics Analysis of GNL3L Expression, Prognosis, and Immune Value in Pan-Cancer |
| title_fullStr | Multi-Omics Analysis of GNL3L Expression, Prognosis, and Immune Value in Pan-Cancer |
| title_full_unstemmed | Multi-Omics Analysis of GNL3L Expression, Prognosis, and Immune Value in Pan-Cancer |
| title_short | Multi-Omics Analysis of GNL3L Expression, Prognosis, and Immune Value in Pan-Cancer |
| title_sort | multi omics analysis of gnl3l expression prognosis and immune value in pan cancer |
| topic | GNL3L pan-cancer multi-omics prognostic analysis immune |
| url | https://www.mdpi.com/2072-6694/14/19/4595 |
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