Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, Brazil
The purpose of this study was to verify the seropositivity of IgG anti-T. gondii antibodies in serum samples and to detect T. gondii DNA detection in tissue from stray cats captured by the Zoonosis Control Center (ZCC) of Goiânia, state of Goiás, during 2016. Antibodies were analyzed by means of an...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Goiás
2019-12-01
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| Series: | Revista de Biologia Neotropical |
| Subjects: | |
| Online Access: | https://revistas.jatai.ufg.br/RBN/article/view/56263 |
| Summary: | The purpose of this study was to verify the seropositivity of IgG anti-T. gondii antibodies in serum samples and to detect T. gondii DNA detection in tissue from stray cats captured by the Zoonosis Control Center (ZCC) of Goiânia, state of Goiás, during 2016. Antibodies were analyzed by means of an indirect hemagglutination assay (IHA), while DNA was detected by polymerase chain reaction (PCR), using primers that amplify the B1 gene in specimens of brain, muscle and heart from the animals. The serological analysis revealed that 87.4% (21/24) of the cats were positive and the molecular test indicated a positivity rate of 75% (18/24). These high rates of serological and molecular detection are worrisome for public health because they confirm the important role cats play in environmental contamination, and hence, in the transmission of toxoplasmosis to humans. |
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| ISSN: | 1807-9652 2178-0579 |