Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, Brazil
The purpose of this study was to verify the seropositivity of IgG anti-T. gondii antibodies in serum samples and to detect T. gondii DNA detection in tissue from stray cats captured by the Zoonosis Control Center (ZCC) of Goiânia, state of Goiás, during 2016. Antibodies were analyzed by means of an...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2019-12-01
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| Series: | Revista de Biologia Neotropical |
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| Online Access: | https://revistas.jatai.ufg.br/RBN/article/view/56263 |
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| author | Hânstter Hállison Alves Rezende Jaqueline Ataíde Silva Lima Antonio Roberto Gomes-Júnior Jade de Oliveira Melo Heloísa Ribeiro Storchilo Taynara Cristina Gomes Marina Clare Vinaud Ana Maria de Castro |
| author_facet | Hânstter Hállison Alves Rezende Jaqueline Ataíde Silva Lima Antonio Roberto Gomes-Júnior Jade de Oliveira Melo Heloísa Ribeiro Storchilo Taynara Cristina Gomes Marina Clare Vinaud Ana Maria de Castro |
| author_sort | Hânstter Hállison Alves Rezende |
| collection | DOAJ |
| description | The purpose of this study was to verify the seropositivity of IgG anti-T. gondii antibodies in serum samples and to detect T. gondii DNA detection in tissue from stray cats captured by the Zoonosis Control Center (ZCC) of Goiânia, state of Goiás, during 2016. Antibodies were analyzed by means of an indirect hemagglutination assay (IHA), while DNA was detected by polymerase chain reaction (PCR), using primers that amplify the B1 gene in specimens of brain, muscle and heart from the animals. The serological analysis revealed that 87.4% (21/24) of the cats were positive and the molecular test indicated a positivity rate of 75% (18/24). These high rates of serological and molecular detection are worrisome for public health because they confirm the important role cats play in environmental contamination, and hence, in the transmission of toxoplasmosis to humans. |
| first_indexed | 2024-12-20T23:43:21Z |
| format | Article |
| id | doaj.art-bb696bcbabfd4213a95c195507cfb8a5 |
| institution | Directory Open Access Journal |
| issn | 1807-9652 2178-0579 |
| language | English |
| last_indexed | 2024-12-20T23:43:21Z |
| publishDate | 2019-12-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Revista de Biologia Neotropical |
| spelling | doaj.art-bb696bcbabfd4213a95c195507cfb8a52022-12-21T19:23:01ZengUniversidade Federal de GoiásRevista de Biologia Neotropical1807-96522178-05792019-12-0116210.5216/rbn.v16i2.56263Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, BrazilHânstter Hállison Alves RezendeJaqueline Ataíde Silva LimaAntonio Roberto Gomes-JúniorJade de Oliveira MeloHeloísa Ribeiro StorchiloTaynara Cristina GomesMarina Clare VinaudAna Maria de CastroThe purpose of this study was to verify the seropositivity of IgG anti-T. gondii antibodies in serum samples and to detect T. gondii DNA detection in tissue from stray cats captured by the Zoonosis Control Center (ZCC) of Goiânia, state of Goiás, during 2016. Antibodies were analyzed by means of an indirect hemagglutination assay (IHA), while DNA was detected by polymerase chain reaction (PCR), using primers that amplify the B1 gene in specimens of brain, muscle and heart from the animals. The serological analysis revealed that 87.4% (21/24) of the cats were positive and the molecular test indicated a positivity rate of 75% (18/24). These high rates of serological and molecular detection are worrisome for public health because they confirm the important role cats play in environmental contamination, and hence, in the transmission of toxoplasmosis to humans.https://revistas.jatai.ufg.br/RBN/article/view/56263Toxoplasmosiscatshemagglutinationpolymerase chain reactionGene |
| spellingShingle | Hânstter Hállison Alves Rezende Jaqueline Ataíde Silva Lima Antonio Roberto Gomes-Júnior Jade de Oliveira Melo Heloísa Ribeiro Storchilo Taynara Cristina Gomes Marina Clare Vinaud Ana Maria de Castro Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, Brazil Revista de Biologia Neotropical Toxoplasmosis cats hemagglutination polymerase chain reaction Gene |
| title | Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, Brazil |
| title_full | Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, Brazil |
| title_fullStr | Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, Brazil |
| title_full_unstemmed | Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, Brazil |
| title_short | Detection of DNA and anti-Toxoplasma gondii antibodies in errant cats (Felis catus domesticus, Linnaeus, 1758) captured by the Zoonoses Control Center of Goiânia, state of Goiás, Brazil |
| title_sort | detection of dna and anti toxoplasma gondii antibodies in errant cats felis catus domesticus linnaeus 1758 captured by the zoonoses control center of goiania state of goias brazil |
| topic | Toxoplasmosis cats hemagglutination polymerase chain reaction Gene |
| url | https://revistas.jatai.ufg.br/RBN/article/view/56263 |
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