Molecular DNA identification of blood sources fed on, for Culicine mosquitoes (Diptera: Culicidae) collected in the Songkhla province, southern Thailand
Culicine mosquitoes are medically important vectors. Therefore, mosquito control measures are a crucial strategy to interrupt disease transmission. Collection of data on mosquito feeding patterns is crucial for developing an effective vector control strategy. The objective of this study was to use...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2017-12-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://rdo.psu.ac.th/sjstweb/journal/39-6/39-6-5.pdf |
| _version_ | 1819118449901699072 |
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| author | Theerakamol Pengsakul Napadol Sudsom Gregory Foakes Kartik Bhatt Marisa Eisenberg Padet Siriyasatien |
| author_facet | Theerakamol Pengsakul Napadol Sudsom Gregory Foakes Kartik Bhatt Marisa Eisenberg Padet Siriyasatien |
| author_sort | Theerakamol Pengsakul |
| collection | DOAJ |
| description | Culicine mosquitoes are medically important vectors. Therefore, mosquito control measures are a crucial strategy to
interrupt disease transmission. Collection of data on mosquito feeding patterns is crucial for developing an effective vector
control strategy. The objective of this study was to use molecular biology methods to identify the sources of DNA in
mosquito blood meals. The DNA from blood meals in the mosquito stomachs was extracted and amplified with multiplex PCR,
using specific primer sets based on the mitochondrial cytochrome b gene, to identify the DNA sources among human, pig,
goat, dog, cow, and chicken. Among the 297 mosquito samples collected in the Songkhla province of Thailand, in Aedes spp.
mosquitoes the percentages positive for human, dog, pig, chicken, cow, a mixture of 2 vertebrate DNAs, or of 3, and negative
(no identified DNA) were 61.90, 2.38, 2.38, 0.60, 0.60, 4.18, 1.20 and 26.79% respectively. In Culex spp. blood meals the rank
order was different: fractions positive for chicken, human, dog, cow, goat, pig, a mixture of 2 or 3 vertebrate DNAs, and
negative were 40.83, 10.00, 5.00, 4.17, 1.67, 0.83, 8.32, 3.32 and 25.83% respectively. This study shows that feeding behaviors
of the two species differ, with most Aedes spp. blood meals containing human blood, while Culex spp. had primarily consumed
chicken blood. An improved understanding of the feeding behaviors of mosquitoes could contribute to new, more effective
strategies for the control of mosquito populations. |
| first_indexed | 2024-12-22T05:49:04Z |
| format | Article |
| id | doaj.art-bb69ad0dea454b42a6e3f80b57b639aa |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-22T05:49:04Z |
| publishDate | 2017-12-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-bb69ad0dea454b42a6e3f80b57b639aa2022-12-21T18:36:56ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952017-12-0139673173710.14456/sjst-psu.2017.89Molecular DNA identification of blood sources fed on, for Culicine mosquitoes (Diptera: Culicidae) collected in the Songkhla province, southern ThailandTheerakamol Pengsakul0Napadol Sudsom1Gregory Foakes2Kartik Bhatt3Marisa Eisenberg4Padet Siriyasatien5Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla, 90110 ThailandNan Provincial Public Health Office, Mueang, Nan, 55000 ThailandDepartments of Epidemiology and Mathematics, School of Public Health, University of Michigan, Ann Arbor, Michigan, 48109 United States of AmericaDepartments of Epidemiology and Mathematics, School of Public Health, University of Michigan, Ann Arbor, Michigan, 48109 United States of AmericaDepartments of Epidemiology and Mathematics, School of Public Health, University of Michigan, Ann Arbor, Michigan, 48109 United States of AmericaDepartment of Parasitology, Faculty of Medicine, Chulalongkorn University, Pathum Wan, Bangkok, 10330 ThailandCulicine mosquitoes are medically important vectors. Therefore, mosquito control measures are a crucial strategy to interrupt disease transmission. Collection of data on mosquito feeding patterns is crucial for developing an effective vector control strategy. The objective of this study was to use molecular biology methods to identify the sources of DNA in mosquito blood meals. The DNA from blood meals in the mosquito stomachs was extracted and amplified with multiplex PCR, using specific primer sets based on the mitochondrial cytochrome b gene, to identify the DNA sources among human, pig, goat, dog, cow, and chicken. Among the 297 mosquito samples collected in the Songkhla province of Thailand, in Aedes spp. mosquitoes the percentages positive for human, dog, pig, chicken, cow, a mixture of 2 vertebrate DNAs, or of 3, and negative (no identified DNA) were 61.90, 2.38, 2.38, 0.60, 0.60, 4.18, 1.20 and 26.79% respectively. In Culex spp. blood meals the rank order was different: fractions positive for chicken, human, dog, cow, goat, pig, a mixture of 2 or 3 vertebrate DNAs, and negative were 40.83, 10.00, 5.00, 4.17, 1.67, 0.83, 8.32, 3.32 and 25.83% respectively. This study shows that feeding behaviors of the two species differ, with most Aedes spp. blood meals containing human blood, while Culex spp. had primarily consumed chicken blood. An improved understanding of the feeding behaviors of mosquitoes could contribute to new, more effective strategies for the control of mosquito populations.http://rdo.psu.ac.th/sjstweb/journal/39-6/39-6-5.pdfcytochrome b genemultiplex PCRAedes spp.Culex spp.blood meal |
| spellingShingle | Theerakamol Pengsakul Napadol Sudsom Gregory Foakes Kartik Bhatt Marisa Eisenberg Padet Siriyasatien Molecular DNA identification of blood sources fed on, for Culicine mosquitoes (Diptera: Culicidae) collected in the Songkhla province, southern Thailand Songklanakarin Journal of Science and Technology (SJST) cytochrome b gene multiplex PCR Aedes spp. Culex spp. blood meal |
| title | Molecular DNA identification of blood sources fed on, for Culicine mosquitoes (Diptera: Culicidae) collected in the Songkhla province, southern Thailand |
| title_full | Molecular DNA identification of blood sources fed on, for Culicine mosquitoes (Diptera: Culicidae) collected in the Songkhla province, southern Thailand |
| title_fullStr | Molecular DNA identification of blood sources fed on, for Culicine mosquitoes (Diptera: Culicidae) collected in the Songkhla province, southern Thailand |
| title_full_unstemmed | Molecular DNA identification of blood sources fed on, for Culicine mosquitoes (Diptera: Culicidae) collected in the Songkhla province, southern Thailand |
| title_short | Molecular DNA identification of blood sources fed on, for Culicine mosquitoes (Diptera: Culicidae) collected in the Songkhla province, southern Thailand |
| title_sort | molecular dna identification of blood sources fed on for culicine mosquitoes diptera culicidae collected in the songkhla province southern thailand |
| topic | cytochrome b gene multiplex PCR Aedes spp. Culex spp. blood meal |
| url | http://rdo.psu.ac.th/sjstweb/journal/39-6/39-6-5.pdf |
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