A Novel Polysaccharide from <i>Auricularia</i><i>Auricula</i> Alleviates Thrombosis Induced by Carrageenan in Mice
The increasing incidence of cardiovascular diseases has created an urgent need for safe and effective antithrombotic agents. In this study, we aimed to elucidate the structural characteristics and antithrombotic activity of a novel polysaccharide isolated from <i>Auricularia</i><i>...
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MDPI AG
2022-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/15/4831 |
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| author | Chun Bian Lanyang Ji Hang Qu Zhenyu Wang |
| author_facet | Chun Bian Lanyang Ji Hang Qu Zhenyu Wang |
| author_sort | Chun Bian |
| collection | DOAJ |
| description | The increasing incidence of cardiovascular diseases has created an urgent need for safe and effective antithrombotic agents. In this study, we aimed to elucidate the structural characteristics and antithrombotic activity of a novel polysaccharide isolated from <i>Auricularia</i><i>auricula</i> fruiting bodies. The purified polysaccharide AAP-b2 (12.02 kDa) was composed of mannose, glucuronic acid, glucose and xylose, with a molar ratio of 89.25:30.50:4.25:1.00. Methylation and NMR analyses showed that AAP-b2 primarily consisted of →2,3)-Manp-(1→, →3)-Manp-(1→, →4)-GlcAp-(1→ and Manp-(1→. A thrombus mouse model induced by carrageenan was used in this research to evaluate its antithrombotic effect. AAP-b2 significantly inhibited platelet aggregation, reduced the black tail length and prolonged the coagulation time, including activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT), exerting a good inhibitory effect on thrombosis in mice. The antithrombotic activity of AAP-b2 was found to be related to the inhibition of platelet activation by regulation of endothelial nitric oxide synthases (eNOs), endothelin-1 (ET-1), prostacyclin (PGI2) and thromboxane B2 (TXB2), along with the enhancement of anticoagulant activity by affecting antithrombin III (AT-III) and protein C (PC) pathways. |
| first_indexed | 2024-03-09T05:10:23Z |
| format | Article |
| id | doaj.art-bb6a99b8a926427195c7129352a0207f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T05:10:23Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bb6a99b8a926427195c7129352a0207f2023-12-03T12:49:56ZengMDPI AGMolecules1420-30492022-07-012715483110.3390/molecules27154831A Novel Polysaccharide from <i>Auricularia</i><i>Auricula</i> Alleviates Thrombosis Induced by Carrageenan in MiceChun Bian0Lanyang Ji1Hang Qu2Zhenyu Wang3School of Food Engineering, Harbin University, Harbin 150036, ChinaHeilongjiang Province Grain Quality & Safety Monitor and Technology Center, Harbin 150008, ChinaSchool of Life & Environmental Science, Wenzhou University, Wenzhou 325035, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150006, ChinaThe increasing incidence of cardiovascular diseases has created an urgent need for safe and effective antithrombotic agents. In this study, we aimed to elucidate the structural characteristics and antithrombotic activity of a novel polysaccharide isolated from <i>Auricularia</i><i>auricula</i> fruiting bodies. The purified polysaccharide AAP-b2 (12.02 kDa) was composed of mannose, glucuronic acid, glucose and xylose, with a molar ratio of 89.25:30.50:4.25:1.00. Methylation and NMR analyses showed that AAP-b2 primarily consisted of →2,3)-Manp-(1→, →3)-Manp-(1→, →4)-GlcAp-(1→ and Manp-(1→. A thrombus mouse model induced by carrageenan was used in this research to evaluate its antithrombotic effect. AAP-b2 significantly inhibited platelet aggregation, reduced the black tail length and prolonged the coagulation time, including activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT), exerting a good inhibitory effect on thrombosis in mice. The antithrombotic activity of AAP-b2 was found to be related to the inhibition of platelet activation by regulation of endothelial nitric oxide synthases (eNOs), endothelin-1 (ET-1), prostacyclin (PGI2) and thromboxane B2 (TXB2), along with the enhancement of anticoagulant activity by affecting antithrombin III (AT-III) and protein C (PC) pathways.https://www.mdpi.com/1420-3049/27/15/4831<i>Auricularia auricula</i>polysaccharidestructure elucidationantithrombotic activitythrombosis |
| spellingShingle | Chun Bian Lanyang Ji Hang Qu Zhenyu Wang A Novel Polysaccharide from <i>Auricularia</i><i>Auricula</i> Alleviates Thrombosis Induced by Carrageenan in Mice Molecules <i>Auricularia auricula</i> polysaccharide structure elucidation antithrombotic activity thrombosis |
| title | A Novel Polysaccharide from <i>Auricularia</i><i>Auricula</i> Alleviates Thrombosis Induced by Carrageenan in Mice |
| title_full | A Novel Polysaccharide from <i>Auricularia</i><i>Auricula</i> Alleviates Thrombosis Induced by Carrageenan in Mice |
| title_fullStr | A Novel Polysaccharide from <i>Auricularia</i><i>Auricula</i> Alleviates Thrombosis Induced by Carrageenan in Mice |
| title_full_unstemmed | A Novel Polysaccharide from <i>Auricularia</i><i>Auricula</i> Alleviates Thrombosis Induced by Carrageenan in Mice |
| title_short | A Novel Polysaccharide from <i>Auricularia</i><i>Auricula</i> Alleviates Thrombosis Induced by Carrageenan in Mice |
| title_sort | novel polysaccharide from i auricularia i i auricula i alleviates thrombosis induced by carrageenan in mice |
| topic | <i>Auricularia auricula</i> polysaccharide structure elucidation antithrombotic activity thrombosis |
| url | https://www.mdpi.com/1420-3049/27/15/4831 |
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