Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries
Existing Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs.
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-25323-x |
| _version_ | 1819194853323440128 |
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| author | Longwei Liu Praopim Limsakul Xianhui Meng Yan Huang Reed E. S. Harrison Tse-Shun Huang Yiwen Shi Yiyan Yu Krit Charupanit Sheng Zhong Shaoying Lu Jin Zhang Shu Chien Jie Sun Yingxiao Wang |
| author_facet | Longwei Liu Praopim Limsakul Xianhui Meng Yan Huang Reed E. S. Harrison Tse-Shun Huang Yiwen Shi Yiyan Yu Krit Charupanit Sheng Zhong Shaoying Lu Jin Zhang Shu Chien Jie Sun Yingxiao Wang |
| author_sort | Longwei Liu |
| collection | DOAJ |
| description | Existing Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs. |
| first_indexed | 2024-12-23T02:03:28Z |
| format | Article |
| id | doaj.art-bb6ff210871349e99294dd89352b8506 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-23T02:03:28Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-bb6ff210871349e99294dd89352b85062022-12-21T18:03:56ZengNature PortfolioNature Communications2041-17232021-08-0112111610.1038/s41467-021-25323-xIntegration of FRET and sequencing to engineer kinase biosensors from mammalian cell librariesLongwei Liu0Praopim Limsakul1Xianhui Meng2Yan Huang3Reed E. S. Harrison4Tse-Shun Huang5Yiwen Shi6Yiyan Yu7Krit Charupanit8Sheng Zhong9Shaoying Lu10Jin Zhang11Shu Chien12Jie Sun13Yingxiao Wang14Department of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Cell Biology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of MedicineDepartment of Chemistry and Chemical Engineering, Hunan UniversityDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla UniversityDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Pharmacology, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Cell Biology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of MedicineDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaExisting Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs.https://doi.org/10.1038/s41467-021-25323-x |
| spellingShingle | Longwei Liu Praopim Limsakul Xianhui Meng Yan Huang Reed E. S. Harrison Tse-Shun Huang Yiwen Shi Yiyan Yu Krit Charupanit Sheng Zhong Shaoying Lu Jin Zhang Shu Chien Jie Sun Yingxiao Wang Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries Nature Communications |
| title | Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_full | Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_fullStr | Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_full_unstemmed | Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_short | Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_sort | integration of fret and sequencing to engineer kinase biosensors from mammalian cell libraries |
| url | https://doi.org/10.1038/s41467-021-25323-x |
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