Antibodies against the Mycobacterium tuberculosis complex and Brucella spp. in captive and free‐living European bison (Bison bonasus) in Poland
Abstract Background The European bison (Bison bonasus), a symbol of Polish nature, is a protected species that requires active health monitoring. However, conservation efforts are made difficult by the zoonotic diseases such as brucellosis and tuberculosis. Objective The aim of this study was to scr...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.1314 |
| _version_ | 1797353760894222336 |
|---|---|
| author | Anna Didkowska Elisa Ferreras Colino Wanda Olech Hugguette Gloddy Krzysztof Anusz José Antonio Infantes‐Lorenzo Christian Gortázar |
| author_facet | Anna Didkowska Elisa Ferreras Colino Wanda Olech Hugguette Gloddy Krzysztof Anusz José Antonio Infantes‐Lorenzo Christian Gortázar |
| author_sort | Anna Didkowska |
| collection | DOAJ |
| description | Abstract Background The European bison (Bison bonasus), a symbol of Polish nature, is a protected species that requires active health monitoring. However, conservation efforts are made difficult by the zoonotic diseases such as brucellosis and tuberculosis. Objective The aim of this study was to screen the Polish European bison population for exposure to the Mycobacterium tuberculosis complex (MTC) and Brucella spp. Methods A total of 323 free‐living and captive European bison from 13 localities were tested serologically for antibodies against the M. bovis P22 multi‐protein complex (in‐house ELISA) and against Brucella spp. (commercial ELISA). Results Antibodies against the MTC (P22) were detected in 7% (22/323) of the tested European bison. Anti‐MTC antibody positivity was not significantly different by sex, age, and captive/free range status. Anti‐MTC antibodies were found in six of 13 populations sampled, always in populations with larger sample sizes including the four free‐living ones. Antibodies against Brucella spp. were detected in 36% (116/323) of the tested bison. While Brucella spp. antibody prevalence was not different by sex, it was significantly different by age (lower in adults) and captive/free‐living status. Brucella spp. seroprevalence decreased with sample size and seropositive bison were found in 12 of 13 sampling populations. Conclusions Our findings identify potential emerging threats to the European bison population and confirm the first serological response to P22 in European bison. As Poland is currently officially free of brucellosis and bovine tuberculosis, our results require careful interpretation. Further studies are needed to establish the presence of cross‐reactions with atypical mycobacteria in the case of MTC and other bacteria (e.g. Yersinia enterocolitica O:9) in the case of Brucella spp. |
| first_indexed | 2024-03-08T13:35:41Z |
| format | Article |
| id | doaj.art-bb7067e25d624328bddb2326136e6353 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-08T13:35:41Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-bb7067e25d624328bddb2326136e63532024-01-16T18:58:32ZengWileyVeterinary Medicine and Science2053-10952024-01-01101n/an/a10.1002/vms3.1314Antibodies against the Mycobacterium tuberculosis complex and Brucella spp. in captive and free‐living European bison (Bison bonasus) in PolandAnna Didkowska0Elisa Ferreras Colino1Wanda Olech2Hugguette Gloddy3Krzysztof Anusz4José Antonio Infantes‐Lorenzo5Christian Gortázar6Department of Food Hygiene and Public Health Protection Institute of Veterinary Medicine Warsaw University of Life Sciences (SGGW) Warsaw PolandSaBio Instituto de Investigación en Recursos Cinegéticos IREC Consejo Superior de Investigaciones Científicas, Universidad de Castilla – La Mancha Ciudad Real SpainDepartment of Animal Genetics and Conservation Institute of Animal Sciences University of Life Sciences (SGGW) Warsaw PolandSaBio Instituto de Investigación en Recursos Cinegéticos IREC Consejo Superior de Investigaciones Científicas, Universidad de Castilla – La Mancha Ciudad Real SpainDepartment of Food Hygiene and Public Health Protection Institute of Veterinary Medicine Warsaw University of Life Sciences (SGGW) Warsaw PolandUnidad de Inmunología Microbiana Centro Nacional de Microbiología Instituto de Salud Carlos III Majadahonda Madrid SpainSaBio Instituto de Investigación en Recursos Cinegéticos IREC Consejo Superior de Investigaciones Científicas, Universidad de Castilla – La Mancha Ciudad Real SpainAbstract Background The European bison (Bison bonasus), a symbol of Polish nature, is a protected species that requires active health monitoring. However, conservation efforts are made difficult by the zoonotic diseases such as brucellosis and tuberculosis. Objective The aim of this study was to screen the Polish European bison population for exposure to the Mycobacterium tuberculosis complex (MTC) and Brucella spp. Methods A total of 323 free‐living and captive European bison from 13 localities were tested serologically for antibodies against the M. bovis P22 multi‐protein complex (in‐house ELISA) and against Brucella spp. (commercial ELISA). Results Antibodies against the MTC (P22) were detected in 7% (22/323) of the tested European bison. Anti‐MTC antibody positivity was not significantly different by sex, age, and captive/free range status. Anti‐MTC antibodies were found in six of 13 populations sampled, always in populations with larger sample sizes including the four free‐living ones. Antibodies against Brucella spp. were detected in 36% (116/323) of the tested bison. While Brucella spp. antibody prevalence was not different by sex, it was significantly different by age (lower in adults) and captive/free‐living status. Brucella spp. seroprevalence decreased with sample size and seropositive bison were found in 12 of 13 sampling populations. Conclusions Our findings identify potential emerging threats to the European bison population and confirm the first serological response to P22 in European bison. As Poland is currently officially free of brucellosis and bovine tuberculosis, our results require careful interpretation. Further studies are needed to establish the presence of cross‐reactions with atypical mycobacteria in the case of MTC and other bacteria (e.g. Yersinia enterocolitica O:9) in the case of Brucella spp.https://doi.org/10.1002/vms3.1314Brucella sppELISAEuropean bisonP22serologytuberculosis |
| spellingShingle | Anna Didkowska Elisa Ferreras Colino Wanda Olech Hugguette Gloddy Krzysztof Anusz José Antonio Infantes‐Lorenzo Christian Gortázar Antibodies against the Mycobacterium tuberculosis complex and Brucella spp. in captive and free‐living European bison (Bison bonasus) in Poland Veterinary Medicine and Science Brucella spp ELISA European bison P22 serology tuberculosis |
| title | Antibodies against the Mycobacterium tuberculosis complex and Brucella spp. in captive and free‐living European bison (Bison bonasus) in Poland |
| title_full | Antibodies against the Mycobacterium tuberculosis complex and Brucella spp. in captive and free‐living European bison (Bison bonasus) in Poland |
| title_fullStr | Antibodies against the Mycobacterium tuberculosis complex and Brucella spp. in captive and free‐living European bison (Bison bonasus) in Poland |
| title_full_unstemmed | Antibodies against the Mycobacterium tuberculosis complex and Brucella spp. in captive and free‐living European bison (Bison bonasus) in Poland |
| title_short | Antibodies against the Mycobacterium tuberculosis complex and Brucella spp. in captive and free‐living European bison (Bison bonasus) in Poland |
| title_sort | antibodies against the mycobacterium tuberculosis complex and brucella spp in captive and free living european bison bison bonasus in poland |
| topic | Brucella spp ELISA European bison P22 serology tuberculosis |
| url | https://doi.org/10.1002/vms3.1314 |
| work_keys_str_mv | AT annadidkowska antibodiesagainstthemycobacteriumtuberculosiscomplexandbrucellasppincaptiveandfreelivingeuropeanbisonbisonbonasusinpoland AT elisaferrerascolino antibodiesagainstthemycobacteriumtuberculosiscomplexandbrucellasppincaptiveandfreelivingeuropeanbisonbisonbonasusinpoland AT wandaolech antibodiesagainstthemycobacteriumtuberculosiscomplexandbrucellasppincaptiveandfreelivingeuropeanbisonbisonbonasusinpoland AT hugguettegloddy antibodiesagainstthemycobacteriumtuberculosiscomplexandbrucellasppincaptiveandfreelivingeuropeanbisonbisonbonasusinpoland AT krzysztofanusz antibodiesagainstthemycobacteriumtuberculosiscomplexandbrucellasppincaptiveandfreelivingeuropeanbisonbisonbonasusinpoland AT joseantonioinfanteslorenzo antibodiesagainstthemycobacteriumtuberculosiscomplexandbrucellasppincaptiveandfreelivingeuropeanbisonbisonbonasusinpoland AT christiangortazar antibodiesagainstthemycobacteriumtuberculosiscomplexandbrucellasppincaptiveandfreelivingeuropeanbisonbisonbonasusinpoland |