First Report of Hemp Fusarium Wilt Caused by <i>Fusarium oxysporum</i> in Croatia
Wilted hemp (<i>Cannabis sativa</i> L.) plants were observed in August 2019 in commercial fields around Osijek, Croatia. Plants and roots with disease symptoms were collected. The single-spored isolates produced septate cottony white to light pink aerial mycelium and purple undersurface...
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MDPI AG
2023-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/18/3305 |
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| author | Tomislav Duvnjak Karolina Vrandecic Aleksandra Sudaric Jasenka Cosic Tamara Siber Maja Matosa Kocar |
| author_facet | Tomislav Duvnjak Karolina Vrandecic Aleksandra Sudaric Jasenka Cosic Tamara Siber Maja Matosa Kocar |
| author_sort | Tomislav Duvnjak |
| collection | DOAJ |
| description | Wilted hemp (<i>Cannabis sativa</i> L.) plants were observed in August 2019 in commercial fields around Osijek, Croatia. Plants and roots with disease symptoms were collected. The single-spored isolates produced septate cottony white to light pink aerial mycelium and purple undersurface on potato dextrose agar (PDA). Smooth and hyaline hyphae were branched and septate. Macroconidia were fusiform to sickle-shaped with foot-shaped basal cells, elongated apical cells and three to five septa. Sequencing of the internal transcribed spacer and the partial elongation factor 1-α gene identified the species as <i>Fusarium oxysporum</i>. Artificial infection fulfills Koch’s postulates, producing plants which show stunted growth and wilt symptoms similar to those observed in the commercial fields. Control seedlings remained symptomless and healthy. To the best of our knowledge, this is the first report of hemp Fusarium wilt causing <i>F. oxysporum</i> in Croatia. Considering that <i>F. oxysporum</i> has been reported in main field crops in Croatia, the presence of this pathogen could cause economically significant hemp production decreases, especially in humid and cold springs and susceptible varieties. |
| first_indexed | 2024-03-10T22:12:43Z |
| format | Article |
| id | doaj.art-bb72d09c10df4e1ab7bb9551a539e91c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T22:12:43Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-bb72d09c10df4e1ab7bb9551a539e91c2023-11-19T12:33:01ZengMDPI AGPlants2223-77472023-09-011218330510.3390/plants12183305First Report of Hemp Fusarium Wilt Caused by <i>Fusarium oxysporum</i> in CroatiaTomislav Duvnjak0Karolina Vrandecic1Aleksandra Sudaric2Jasenka Cosic3Tamara Siber4Maja Matosa Kocar5Department of Industrial Plants Breeding and Genetics, Agricultural Institute Osijek, 31000 Osijek, CroatiaDepartment of Phytomedicine, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, CroatiaDepartment of Industrial Plants Breeding and Genetics, Agricultural Institute Osijek, 31000 Osijek, CroatiaDepartment of Phytomedicine, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, CroatiaDepartment of Phytomedicine, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, CroatiaDepartment of Industrial Plants Breeding and Genetics, Agricultural Institute Osijek, 31000 Osijek, CroatiaWilted hemp (<i>Cannabis sativa</i> L.) plants were observed in August 2019 in commercial fields around Osijek, Croatia. Plants and roots with disease symptoms were collected. The single-spored isolates produced septate cottony white to light pink aerial mycelium and purple undersurface on potato dextrose agar (PDA). Smooth and hyaline hyphae were branched and septate. Macroconidia were fusiform to sickle-shaped with foot-shaped basal cells, elongated apical cells and three to five septa. Sequencing of the internal transcribed spacer and the partial elongation factor 1-α gene identified the species as <i>Fusarium oxysporum</i>. Artificial infection fulfills Koch’s postulates, producing plants which show stunted growth and wilt symptoms similar to those observed in the commercial fields. Control seedlings remained symptomless and healthy. To the best of our knowledge, this is the first report of hemp Fusarium wilt causing <i>F. oxysporum</i> in Croatia. Considering that <i>F. oxysporum</i> has been reported in main field crops in Croatia, the presence of this pathogen could cause economically significant hemp production decreases, especially in humid and cold springs and susceptible varieties.https://www.mdpi.com/2223-7747/12/18/3305hemp<i>Fusarium oxysporum</i>wiltCroatia |
| spellingShingle | Tomislav Duvnjak Karolina Vrandecic Aleksandra Sudaric Jasenka Cosic Tamara Siber Maja Matosa Kocar First Report of Hemp Fusarium Wilt Caused by <i>Fusarium oxysporum</i> in Croatia Plants hemp <i>Fusarium oxysporum</i> wilt Croatia |
| title | First Report of Hemp Fusarium Wilt Caused by <i>Fusarium oxysporum</i> in Croatia |
| title_full | First Report of Hemp Fusarium Wilt Caused by <i>Fusarium oxysporum</i> in Croatia |
| title_fullStr | First Report of Hemp Fusarium Wilt Caused by <i>Fusarium oxysporum</i> in Croatia |
| title_full_unstemmed | First Report of Hemp Fusarium Wilt Caused by <i>Fusarium oxysporum</i> in Croatia |
| title_short | First Report of Hemp Fusarium Wilt Caused by <i>Fusarium oxysporum</i> in Croatia |
| title_sort | first report of hemp fusarium wilt caused by i fusarium oxysporum i in croatia |
| topic | hemp <i>Fusarium oxysporum</i> wilt Croatia |
| url | https://www.mdpi.com/2223-7747/12/18/3305 |
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