Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria
Abstract Background Streptococcus agalactiae (Group B Streptococcus, GBS) is one of the major bacterial pathogens responsible for neonatal sepsis. Whole genome sequencing has, in recent years, emerged as a reliable tool for capsular typing and antimicrobial resistance prediction. This study characte...
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| Format: | Article |
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BMC
2021-08-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07929-z |
| _version_ | 1818881069611483136 |
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| author | Mienye Bob-Manuel Lesley McGee Jeremiah A Igunma Mary A Alex-Wele Orikomaba K Obunge Kennedy T Wariso |
| author_facet | Mienye Bob-Manuel Lesley McGee Jeremiah A Igunma Mary A Alex-Wele Orikomaba K Obunge Kennedy T Wariso |
| author_sort | Mienye Bob-Manuel |
| collection | DOAJ |
| description | Abstract Background Streptococcus agalactiae (Group B Streptococcus, GBS) is one of the major bacterial pathogens responsible for neonatal sepsis. Whole genome sequencing has, in recent years, emerged as a reliable tool for capsular typing and antimicrobial resistance prediction. This study characterized vaginal and rectal isolates of Group B Streptococcus obtained from pregnant women in Port Harcourt, Nigeria using a whole-genome sequence-based approach. Results Capsular types Ia, Ib, II, III, IV and V were detected among the 43 isolates sequenced. Twelve sequence types (STs) were identified, with ST19 (n = 9, 27.3 %) and ST486 (n = 5, 15.2 %) the most frequent among non-duplicated isolates. Of the alpha-like proteins (alp) identified, Alp1 was the most prevalent in 11 (33.3 %) isolates. Macrolide and lincosamide resistance determinants were present in 15 (45.5 %) isolates; ermB was detected in 1 (3 %), ermTR in 7 (21.2 %) isolates, lnu gene was detected in 6 (18.2 %) and mef was identified in 3 (9.1 %) isolates. Resistance of GBS to erythromycin and clindamycin (predicted from presence of erm or mef genes) was found to be 30.3 % and 24.2 %, respectively. All isolates were predicted resistant to tetracycline with only the tetM gene identified. Fluoroquinolone-resistance conferring substitutions in gyrA + parC were detected in 9 (27.3 %) isolates and chloramphenicol resistance was predicted from presence of aac6’-aph2 gene in 11 (33.3 %). Conclusions The data available from the whole genome sequencing of these isolates offers a small but insightful description of common serotypes and resistance features within colonizing GBS in Nigeria. |
| first_indexed | 2024-12-19T14:56:00Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-19T14:56:00Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-bb74d5e7ed1e4e6b8c2b803c988b65b92022-12-21T20:16:43ZengBMCBMC Genomics1471-21642021-08-012211610.1186/s12864-021-07929-zWhole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in NigeriaMienye Bob-Manuel0Lesley McGee1Jeremiah A Igunma2Mary A Alex-Wele3Orikomaba K Obunge4Kennedy T Wariso5Department of Medical Microbiology and Parasitology, Rivers State University Teaching HospitalRespiratory Diseases Branch, Centers for Disease Control and PreventionDepartment of Medical Microbiology, University of Benin Teaching HospitalDepartment of Medical Microbiology and Parasitology, University of Port Harcourt Teaching HospitalDepartment of Medical Microbiology and Parasitology, University of Port Harcourt Teaching HospitalDepartment of Medical Microbiology and Parasitology, University of Port Harcourt Teaching HospitalAbstract Background Streptococcus agalactiae (Group B Streptococcus, GBS) is one of the major bacterial pathogens responsible for neonatal sepsis. Whole genome sequencing has, in recent years, emerged as a reliable tool for capsular typing and antimicrobial resistance prediction. This study characterized vaginal and rectal isolates of Group B Streptococcus obtained from pregnant women in Port Harcourt, Nigeria using a whole-genome sequence-based approach. Results Capsular types Ia, Ib, II, III, IV and V were detected among the 43 isolates sequenced. Twelve sequence types (STs) were identified, with ST19 (n = 9, 27.3 %) and ST486 (n = 5, 15.2 %) the most frequent among non-duplicated isolates. Of the alpha-like proteins (alp) identified, Alp1 was the most prevalent in 11 (33.3 %) isolates. Macrolide and lincosamide resistance determinants were present in 15 (45.5 %) isolates; ermB was detected in 1 (3 %), ermTR in 7 (21.2 %) isolates, lnu gene was detected in 6 (18.2 %) and mef was identified in 3 (9.1 %) isolates. Resistance of GBS to erythromycin and clindamycin (predicted from presence of erm or mef genes) was found to be 30.3 % and 24.2 %, respectively. All isolates were predicted resistant to tetracycline with only the tetM gene identified. Fluoroquinolone-resistance conferring substitutions in gyrA + parC were detected in 9 (27.3 %) isolates and chloramphenicol resistance was predicted from presence of aac6’-aph2 gene in 11 (33.3 %). Conclusions The data available from the whole genome sequencing of these isolates offers a small but insightful description of common serotypes and resistance features within colonizing GBS in Nigeria.https://doi.org/10.1186/s12864-021-07929-zWhole genome sequencingGroup B StreptococcusNigeriacapsular typingAntimicrobial resistance |
| spellingShingle | Mienye Bob-Manuel Lesley McGee Jeremiah A Igunma Mary A Alex-Wele Orikomaba K Obunge Kennedy T Wariso Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria BMC Genomics Whole genome sequencing Group B Streptococcus Nigeria capsular typing Antimicrobial resistance |
| title | Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria |
| title_full | Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria |
| title_fullStr | Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria |
| title_full_unstemmed | Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria |
| title_short | Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria |
| title_sort | whole genome sequence based capsular typing and antimicrobial resistance prediction of group b streptococcal isolates from colonized pregnant women in nigeria |
| topic | Whole genome sequencing Group B Streptococcus Nigeria capsular typing Antimicrobial resistance |
| url | https://doi.org/10.1186/s12864-021-07929-z |
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