Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii
We have previously reported that the RNA-binding protein Dull slicer 16 (DUS16) plays a key role in the processing of primary miRNAs (pri-miRNAs) in the unicellular green alga Chlamydomonas reinhardtii. In the present report, we elaborate on the interaction of DUS16 with Dicer-like 3 (DCL3) during p...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Communicative & Integrative Biology |
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| Online Access: | http://dx.doi.org/10.1080/19420889.2017.1280208 |
| _version_ | 1818862540520685568 |
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| author | Tomohito Yamasaki Heriberto Cerutti |
| author_facet | Tomohito Yamasaki Heriberto Cerutti |
| author_sort | Tomohito Yamasaki |
| collection | DOAJ |
| description | We have previously reported that the RNA-binding protein Dull slicer 16 (DUS16) plays a key role in the processing of primary miRNAs (pri-miRNAs) in the unicellular green alga Chlamydomonas reinhardtii. In the present report, we elaborate on the interaction of DUS16 with Dicer-like 3 (DCL3) during pri-miRNA processing. Comprehensive analyses of small RNA libraries derived from mutant and wild-type algal strains allowed the de novo prediction of 35 pri-miRNA genes, including 9 previously unknown ones. The pri-miRNAs dependent on DUS16 for processing largely overlapped with those dependent on DCL3. Our findings suggest that DUS16 and DCL3 work cooperatively, presumably as components of a microprocessor complex, in the processing of the majority of pri-miRNAs in C. reinhardtii. |
| first_indexed | 2024-12-19T10:01:29Z |
| format | Article |
| id | doaj.art-bb77cba06c954c1ca01f98d63603ff96 |
| institution | Directory Open Access Journal |
| issn | 1942-0889 |
| language | English |
| last_indexed | 2024-12-19T10:01:29Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Communicative & Integrative Biology |
| spelling | doaj.art-bb77cba06c954c1ca01f98d63603ff962022-12-21T20:26:39ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892017-01-0110110.1080/19420889.2017.12802081280208Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtiiTomohito Yamasaki0Heriberto Cerutti1National Institute for Basic BiologySchool of Biological Science and Center for Plant Science Innovation, University of Nebraska–LincolnWe have previously reported that the RNA-binding protein Dull slicer 16 (DUS16) plays a key role in the processing of primary miRNAs (pri-miRNAs) in the unicellular green alga Chlamydomonas reinhardtii. In the present report, we elaborate on the interaction of DUS16 with Dicer-like 3 (DCL3) during pri-miRNA processing. Comprehensive analyses of small RNA libraries derived from mutant and wild-type algal strains allowed the de novo prediction of 35 pri-miRNA genes, including 9 previously unknown ones. The pri-miRNAs dependent on DUS16 for processing largely overlapped with those dependent on DCL3. Our findings suggest that DUS16 and DCL3 work cooperatively, presumably as components of a microprocessor complex, in the processing of the majority of pri-miRNAs in C. reinhardtii.http://dx.doi.org/10.1080/19420889.2017.1280208ArgonauteChlamydomonas reinhardtiiDicermiRNARNA-binding proteinsmall RNA-seq |
| spellingShingle | Tomohito Yamasaki Heriberto Cerutti Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii Communicative & Integrative Biology Argonaute Chlamydomonas reinhardtii Dicer miRNA RNA-binding protein small RNA-seq |
| title | Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii |
| title_full | Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii |
| title_fullStr | Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii |
| title_full_unstemmed | Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii |
| title_short | Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii |
| title_sort | cooperative processing of primary mirnas by dus16 and dcl3 in the unicellular green alga chlamydomonas reinhardtii |
| topic | Argonaute Chlamydomonas reinhardtii Dicer miRNA RNA-binding protein small RNA-seq |
| url | http://dx.doi.org/10.1080/19420889.2017.1280208 |
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