Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of <i>Camelus dromedarius</i>
Mesenchymal stem cells (MSCs) are promising multipotent cells with applications for cartilage tissue regeneration in stem cell-based therapies. In cartilage regeneration, both bone marrow (BM-MSCs) and synovial fluid (SF-MSCs) are valuable sources. However, the cellular characteristics and chondrocy...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/7/1918 |
| _version_ | 1797528550962626560 |
|---|---|
| author | Young-Bum Son Yeon Ik Jeong Yeon Woo Jeong Mohammad Shamim Hossein Per Olof Olsson Alex Tinson Kuhad Kuldip Singh Sang-Yun Lee Woo Suk Hwang |
| author_facet | Young-Bum Son Yeon Ik Jeong Yeon Woo Jeong Mohammad Shamim Hossein Per Olof Olsson Alex Tinson Kuhad Kuldip Singh Sang-Yun Lee Woo Suk Hwang |
| author_sort | Young-Bum Son |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are promising multipotent cells with applications for cartilage tissue regeneration in stem cell-based therapies. In cartilage regeneration, both bone marrow (BM-MSCs) and synovial fluid (SF-MSCs) are valuable sources. However, the cellular characteristics and chondrocyte differentiation potential were not reported in either of the camel stem cells. The in vitro chondrocyte differentiation competence of MSCs, from (BM and SF) sources of the same <i>Camelus dromedaries</i> (camel) donor, was determined. Both MSCs were evaluated on pluripotent markers and proliferation capacity. After passage three, both MSCs showed fibroblast-like morphology. The proliferation capacity was significantly increased in SF-MSCs compared to BM-MSCs. Furthermore, SF-MSCs showed an enhanced expression of transcription factors than BM-MSCs. SF-MSCs exhibited lower differentiation potential toward adipocytes than BM-MSCs. However, the osteoblast differentiation potential was similar in MSCs from both sources. Chondrogenic pellets obtained from SF-MSCs revealed higher levels of chondrocyte-specific markers than those from BM-MSCs. Additionally, glycosaminoglycan (GAG) content was elevated in SF-MSCs related to BM-MSCs. This is, to our knowledge, the first study to establish BM-MSCs and SF-MSCs from the same donor and to demonstrate in vitro differentiation potential into chondrocytes in camels. |
| first_indexed | 2024-03-10T10:01:00Z |
| format | Article |
| id | doaj.art-bb78a9e6016543008414d5b1a56b0ee2 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:01:00Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-bb78a9e6016543008414d5b1a56b0ee22023-11-22T02:00:05ZengMDPI AGAnimals2076-26152021-06-01117191810.3390/ani11071918Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of <i>Camelus dromedarius</i>Young-Bum Son0Yeon Ik Jeong1Yeon Woo Jeong2Mohammad Shamim Hossein3Per Olof Olsson4Alex Tinson5Kuhad Kuldip Singh6Sang-Yun Lee7Woo Suk Hwang8UAE Biotech Research Center, Abu Dhabi 30310, United Arab EmiratesUAE Biotech Research Center, Abu Dhabi 30310, United Arab EmiratesUAE Biotech Research Center, Abu Dhabi 30310, United Arab EmiratesUAE Biotech Research Center, Abu Dhabi 30310, United Arab EmiratesUAE Biotech Research Center, Abu Dhabi 30310, United Arab EmiratesHilli E.T. Cloning and Surgical Centre Presidential Camels and Camel Racing Affairs, Al-Ain 17292, United Arab EmiratesHilli E.T. Cloning and Surgical Centre Presidential Camels and Camel Racing Affairs, Al-Ain 17292, United Arab EmiratesDepartment of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, Jinju 52828, KoreaUAE Biotech Research Center, Abu Dhabi 30310, United Arab EmiratesMesenchymal stem cells (MSCs) are promising multipotent cells with applications for cartilage tissue regeneration in stem cell-based therapies. In cartilage regeneration, both bone marrow (BM-MSCs) and synovial fluid (SF-MSCs) are valuable sources. However, the cellular characteristics and chondrocyte differentiation potential were not reported in either of the camel stem cells. The in vitro chondrocyte differentiation competence of MSCs, from (BM and SF) sources of the same <i>Camelus dromedaries</i> (camel) donor, was determined. Both MSCs were evaluated on pluripotent markers and proliferation capacity. After passage three, both MSCs showed fibroblast-like morphology. The proliferation capacity was significantly increased in SF-MSCs compared to BM-MSCs. Furthermore, SF-MSCs showed an enhanced expression of transcription factors than BM-MSCs. SF-MSCs exhibited lower differentiation potential toward adipocytes than BM-MSCs. However, the osteoblast differentiation potential was similar in MSCs from both sources. Chondrogenic pellets obtained from SF-MSCs revealed higher levels of chondrocyte-specific markers than those from BM-MSCs. Additionally, glycosaminoglycan (GAG) content was elevated in SF-MSCs related to BM-MSCs. This is, to our knowledge, the first study to establish BM-MSCs and SF-MSCs from the same donor and to demonstrate in vitro differentiation potential into chondrocytes in camels.https://www.mdpi.com/2076-2615/11/7/1918mesenchymal stem cells<i>Camelus dromedarius</i>bone marrowsynovial fluidchondrocyte differentiation |
| spellingShingle | Young-Bum Son Yeon Ik Jeong Yeon Woo Jeong Mohammad Shamim Hossein Per Olof Olsson Alex Tinson Kuhad Kuldip Singh Sang-Yun Lee Woo Suk Hwang Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of <i>Camelus dromedarius</i> Animals mesenchymal stem cells <i>Camelus dromedarius</i> bone marrow synovial fluid chondrocyte differentiation |
| title | Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of <i>Camelus dromedarius</i> |
| title_full | Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of <i>Camelus dromedarius</i> |
| title_fullStr | Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of <i>Camelus dromedarius</i> |
| title_full_unstemmed | Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of <i>Camelus dromedarius</i> |
| title_short | Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of <i>Camelus dromedarius</i> |
| title_sort | cell source dependent in vitro chondrogenic differentiation potential of mesenchymal stem cell established from bone marrow and synovial fluid of i camelus dromedarius i |
| topic | mesenchymal stem cells <i>Camelus dromedarius</i> bone marrow synovial fluid chondrocyte differentiation |
| url | https://www.mdpi.com/2076-2615/11/7/1918 |
| work_keys_str_mv | AT youngbumson cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi AT yeonikjeong cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi AT yeonwoojeong cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi AT mohammadshamimhossein cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi AT perolofolsson cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi AT alextinson cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi AT kuhadkuldipsingh cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi AT sangyunlee cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi AT woosukhwang cellsourcedependentinvitrochondrogenicdifferentiationpotentialofmesenchymalstemcellestablishedfrombonemarrowandsynovialfluidoficamelusdromedariusi |