Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties
Abstract Background The peanut is one of the most important oil crops worldwide. Qualities and yields of peanut can be dramatically diminished by abiotic stresses particularly by drought. Therefore, it would be beneficial to gain a comprehensive understanding on peanut drought-responsive transcripti...
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| Format: | Article |
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BMC
2021-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-020-02761-1 |
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| author | Chunji Jiang Xinlin Li Jixiang Zou Jingyao Ren Chunyi Jin He Zhang Haiqiu Yu Hua Jin |
| author_facet | Chunji Jiang Xinlin Li Jixiang Zou Jingyao Ren Chunyi Jin He Zhang Haiqiu Yu Hua Jin |
| author_sort | Chunji Jiang |
| collection | DOAJ |
| description | Abstract Background The peanut is one of the most important oil crops worldwide. Qualities and yields of peanut can be dramatically diminished by abiotic stresses particularly by drought. Therefore, it would be beneficial to gain a comprehensive understanding on peanut drought-responsive transcriptional regulatory activities, and hopefully to extract critical drought-tolerance-related molecular mechanism from it. Results In this study, two peanut Arachis hypogaea L. varieties, NH5 (tolerant) and FH18 (sensitive), which show significantly differential drought tolerance, were screened from 23 main commercial peanut cultivars and used for physiological characterization and transcriptomic analysis. NH5 leaves showed higher water and GSH contents, faster stomatal closure, and lower relative conductivity (REC) than FH18. Under the time-course of drought-treatments 0 h (CK), 4 h (DT1), 8 h (DT2) and 24 h (DT3), the number of down-regulated differential expressed genes (DEGs) increased with the progression of treatments indicating repressive impacts on transcriptomes by drought in both peanut varieties. Conclusions Nevertheless, NH5 maintained more stable transcriptomic dynamics than FH18. Furthermore, annotations of identified DEGs implicate signal transduction, the elimination of reactive oxygen species, and the maintenance of cell osmotic potential which are key drought-tolerance-related pathways. Finally, evidences from the examination of ABA and SA components suggested that the fast stomatal closure in NH5 was likely mediated through SA rather than ABA signaling. In all, these results have provided us a comprehensive overview of peanut drought-responsive transcriptomic changes, which could serve as solid foundation for further identification of the molecular drought-tolerance mechanism in peanut and other oil crops. |
| first_indexed | 2024-12-16T16:41:07Z |
| format | Article |
| id | doaj.art-bb7c8b720f6940ad965dade68ef7900f |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-16T16:41:07Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-bb7c8b720f6940ad965dade68ef7900f2022-12-21T22:24:19ZengBMCBMC Plant Biology1471-22292021-01-0121111410.1186/s12870-020-02761-1Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varietiesChunji Jiang0Xinlin Li1Jixiang Zou2Jingyao Ren3Chunyi Jin4He Zhang5Haiqiu Yu6Hua Jin7College of Agriculture, Shenyang Agricultural UniversityCollege of Environment and Resources, Dalian Minzu UniversityCollege of Environment and Resources, Dalian Minzu UniversityCollege of Agriculture, Shenyang Agricultural UniversityCollege of Environment and Resources, Dalian Minzu UniversityCollege of Agriculture, Shenyang Agricultural UniversityCollege of Agriculture, Shenyang Agricultural UniversityCollege of Environment and Resources, Dalian Minzu UniversityAbstract Background The peanut is one of the most important oil crops worldwide. Qualities and yields of peanut can be dramatically diminished by abiotic stresses particularly by drought. Therefore, it would be beneficial to gain a comprehensive understanding on peanut drought-responsive transcriptional regulatory activities, and hopefully to extract critical drought-tolerance-related molecular mechanism from it. Results In this study, two peanut Arachis hypogaea L. varieties, NH5 (tolerant) and FH18 (sensitive), which show significantly differential drought tolerance, were screened from 23 main commercial peanut cultivars and used for physiological characterization and transcriptomic analysis. NH5 leaves showed higher water and GSH contents, faster stomatal closure, and lower relative conductivity (REC) than FH18. Under the time-course of drought-treatments 0 h (CK), 4 h (DT1), 8 h (DT2) and 24 h (DT3), the number of down-regulated differential expressed genes (DEGs) increased with the progression of treatments indicating repressive impacts on transcriptomes by drought in both peanut varieties. Conclusions Nevertheless, NH5 maintained more stable transcriptomic dynamics than FH18. Furthermore, annotations of identified DEGs implicate signal transduction, the elimination of reactive oxygen species, and the maintenance of cell osmotic potential which are key drought-tolerance-related pathways. Finally, evidences from the examination of ABA and SA components suggested that the fast stomatal closure in NH5 was likely mediated through SA rather than ABA signaling. In all, these results have provided us a comprehensive overview of peanut drought-responsive transcriptomic changes, which could serve as solid foundation for further identification of the molecular drought-tolerance mechanism in peanut and other oil crops.https://doi.org/10.1186/s12870-020-02761-1RNA-seqPhytohormonepeanut Arachis hypogaea L.Drought stress |
| spellingShingle | Chunji Jiang Xinlin Li Jixiang Zou Jingyao Ren Chunyi Jin He Zhang Haiqiu Yu Hua Jin Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties BMC Plant Biology RNA-seq Phytohormone peanut Arachis hypogaea L. Drought stress |
| title | Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties |
| title_full | Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties |
| title_fullStr | Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties |
| title_full_unstemmed | Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties |
| title_short | Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties |
| title_sort | comparative transcriptome analysis of genes involved in the drought stress response of two peanut arachis hypogaea l varieties |
| topic | RNA-seq Phytohormone peanut Arachis hypogaea L. Drought stress |
| url | https://doi.org/10.1186/s12870-020-02761-1 |
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