The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1 assays
Abstract Background Soluble programmed death-1 (sPD-1) is a novel immune markers and possibly predictive of chronic hepatitis B (CHB) outcome. However, results were inconsistent by different ELISA kits. This study aims to compare the characteristics and correlations with other markers for sPD-1 meas...
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-022-01777-3 |
| _version_ | 1811280250878820352 |
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| author | Wen-Juei Jeng Chien-Hung Chen Yi-Wen Wang Mei-Hung Pan Chia-Wei Lin Chun-Yen Lin Hwai-I Yang |
| author_facet | Wen-Juei Jeng Chien-Hung Chen Yi-Wen Wang Mei-Hung Pan Chia-Wei Lin Chun-Yen Lin Hwai-I Yang |
| author_sort | Wen-Juei Jeng |
| collection | DOAJ |
| description | Abstract Background Soluble programmed death-1 (sPD-1) is a novel immune markers and possibly predictive of chronic hepatitis B (CHB) outcome. However, results were inconsistent by different ELISA kits. This study aims to compare the characteristics and correlations with other markers for sPD-1 measured by MyBioSource (MB) and R&D (RD) kits. Methods A total of 254 untreated CHB patients from three sites were assayed with sPD-1 by MB and RD kits at the same time. Spearman’s correlations between the kits, and those with viral markers and ALT levels were calculated. Multivariate linear regression analysis was applied for independent factors associated with the sPD-1 levels. Results There’s no correlation between sPD-1 level using MB and RD assays. sPD-1 by MB correlated profoundly with HBsAg (r = 0.8311, P < 0.0001), HBV DNA (r = 0.3896, P < 0.0001), and ALT levels (r = 0.1604, P = 0.0105) while an opposite trend by RD kit (r = − 0.0644, P = 0.3109; r = 0.2554, P < 0.0001; r = 0.4417, P < 0.0001, respectively for the 3 markers). In the multivariate linear regression analysis, HBsAg and ALT levels was the major factor associated with sPD-1 levels by MB and RD, respectively. Conclusions The characteristics and correlations with host/viral markers of sPD-1 by the two kits are different and leading to different associations on clinical outcomes of CHB. |
| first_indexed | 2024-04-13T01:10:16Z |
| format | Article |
| id | doaj.art-bb7ca646633748cbbc58f73715df1486 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-13T01:10:16Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-bb7ca646633748cbbc58f73715df14862022-12-22T03:09:11ZengBMCVirology Journal1743-422X2022-03-011911810.1186/s12985-022-01777-3The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1 assaysWen-Juei Jeng0Chien-Hung Chen1Yi-Wen Wang2Mei-Hung Pan3Chia-Wei Lin4Chun-Yen Lin5Hwai-I Yang6Department of Gastroenterology and Hepatology, Chang Gung Memorial HospitalDepartment of Gastroenterology and Hepatology, Chang Gung Memorial HospitalGenomics Research Center, Academia SinicaGenomics Research Center, Academia SinicaDepartment of Gastroenterology and Hepatology, Chang Gung Memorial HospitalDepartment of Gastroenterology and Hepatology, Chang Gung Memorial HospitalGenomics Research Center, Academia SinicaAbstract Background Soluble programmed death-1 (sPD-1) is a novel immune markers and possibly predictive of chronic hepatitis B (CHB) outcome. However, results were inconsistent by different ELISA kits. This study aims to compare the characteristics and correlations with other markers for sPD-1 measured by MyBioSource (MB) and R&D (RD) kits. Methods A total of 254 untreated CHB patients from three sites were assayed with sPD-1 by MB and RD kits at the same time. Spearman’s correlations between the kits, and those with viral markers and ALT levels were calculated. Multivariate linear regression analysis was applied for independent factors associated with the sPD-1 levels. Results There’s no correlation between sPD-1 level using MB and RD assays. sPD-1 by MB correlated profoundly with HBsAg (r = 0.8311, P < 0.0001), HBV DNA (r = 0.3896, P < 0.0001), and ALT levels (r = 0.1604, P = 0.0105) while an opposite trend by RD kit (r = − 0.0644, P = 0.3109; r = 0.2554, P < 0.0001; r = 0.4417, P < 0.0001, respectively for the 3 markers). In the multivariate linear regression analysis, HBsAg and ALT levels was the major factor associated with sPD-1 levels by MB and RD, respectively. Conclusions The characteristics and correlations with host/viral markers of sPD-1 by the two kits are different and leading to different associations on clinical outcomes of CHB.https://doi.org/10.1186/s12985-022-01777-3Immune markersPD-1AssayHepatitis B virus |
| spellingShingle | Wen-Juei Jeng Chien-Hung Chen Yi-Wen Wang Mei-Hung Pan Chia-Wei Lin Chun-Yen Lin Hwai-I Yang The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1 assays Virology Journal Immune markers PD-1 Assay Hepatitis B virus |
| title | The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1 assays |
| title_full | The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1 assays |
| title_fullStr | The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1 assays |
| title_full_unstemmed | The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1 assays |
| title_short | The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1 assays |
| title_sort | association between spd 1 levels versus liver biochemistry and viral markers in chronic hepatitis b patients a comparative study of different spd 1 assays |
| topic | Immune markers PD-1 Assay Hepatitis B virus |
| url | https://doi.org/10.1186/s12985-022-01777-3 |
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