Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array
Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distin...
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| Format: | Article |
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MDPI AG
2018-03-01
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| Series: | Micromachines |
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| Online Access: | http://www.mdpi.com/2072-666X/9/3/106 |
| _version_ | 1817988530204311552 |
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| author | Yousang Yoon Jusin Lee Ki-Chun Yoo Onejae Sul Su-Jae Lee Seung-Beck Lee |
| author_facet | Yousang Yoon Jusin Lee Ki-Chun Yoo Onejae Sul Su-Jae Lee Seung-Beck Lee |
| author_sort | Yousang Yoon |
| collection | DOAJ |
| description | Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distinct sub-population of CTCs that causes metastasis. Here, we report a flow-restricted microfluidic trap array capable of deterministic single-cell capture of CTCs. The extent of flow restriction, correlating with the device geometry, was then optimized using a highly invasive breast cancer cell line (LM2 MDA-MB-231) to achieve 97% capture efficiency with a single-cell capture rate of 99%. Single-cell capture of CTCs from mice with full-blown metastasis was also demonstrated. The single-CTC capturing ability of the flow-restricted trap array not only showed cell enumerating ability but also high prospects for application in future automated downstream analysis. |
| first_indexed | 2024-04-14T00:36:06Z |
| format | Article |
| id | doaj.art-bb7cf2bb22004a9da31bc70f51f3efd4 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-04-14T00:36:06Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-bb7cf2bb22004a9da31bc70f51f3efd42022-12-22T02:22:23ZengMDPI AGMicromachines2072-666X2018-03-019310610.3390/mi9030106mi9030106Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap ArrayYousang Yoon0Jusin Lee1Ki-Chun Yoo2Onejae Sul3Su-Jae Lee4Seung-Beck Lee5Department of Electronic Engineering, Hanyang Universtiy College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang Universtiy College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Life Science and Research Institute for Natural Sciences, Hanyang Universtiy College of Natural Sciences, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaInstitute of Nano Science and Technology, Hanyang Universtiy, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Life Science and Research Institute for Natural Sciences, Hanyang Universtiy College of Natural Sciences, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Electronic Engineering, Hanyang Universtiy College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaCirculating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distinct sub-population of CTCs that causes metastasis. Here, we report a flow-restricted microfluidic trap array capable of deterministic single-cell capture of CTCs. The extent of flow restriction, correlating with the device geometry, was then optimized using a highly invasive breast cancer cell line (LM2 MDA-MB-231) to achieve 97% capture efficiency with a single-cell capture rate of 99%. Single-cell capture of CTCs from mice with full-blown metastasis was also demonstrated. The single-CTC capturing ability of the flow-restricted trap array not only showed cell enumerating ability but also high prospects for application in future automated downstream analysis.http://www.mdpi.com/2072-666X/9/3/106circulating tumor cellsingle-cell capturemicrofluidics |
| spellingShingle | Yousang Yoon Jusin Lee Ki-Chun Yoo Onejae Sul Su-Jae Lee Seung-Beck Lee Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array Micromachines circulating tumor cell single-cell capture microfluidics |
| title | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
| title_full | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
| title_fullStr | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
| title_full_unstemmed | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
| title_short | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
| title_sort | deterministic capture of individual circulating tumor cells using a flow restricted microfluidic trap array |
| topic | circulating tumor cell single-cell capture microfluidics |
| url | http://www.mdpi.com/2072-666X/9/3/106 |
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