Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry
Production of biofuel from lignocellulosic biomass is relatively low due to the limited knowledge about natural cell wall loosening and cellulolytic processes in plants. Industrial separation of cellulose fiber mass from lignin, its saccharification and alcoholic fermentation is still cost-ineffecti...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/12/16/2018 |
| _version_ | 1797585202319458304 |
|---|---|
| author | Maciej Jerzy Bernacki Jakub Mielecki Andrzej Antczak Michał Drożdżek Damian Witoń Joanna Dąbrowska-Bronk Piotr Gawroński Paweł Burdiak Monika Marchwicka Anna Rusaczonek Katarzyna Dąbkowska-Susfał Wacław Roman Strobel Ewa J. Mellerowicz Janusz Zawadzki Magdalena Szechyńska-Hebda Stanisław Karpiński |
| author_facet | Maciej Jerzy Bernacki Jakub Mielecki Andrzej Antczak Michał Drożdżek Damian Witoń Joanna Dąbrowska-Bronk Piotr Gawroński Paweł Burdiak Monika Marchwicka Anna Rusaczonek Katarzyna Dąbkowska-Susfał Wacław Roman Strobel Ewa J. Mellerowicz Janusz Zawadzki Magdalena Szechyńska-Hebda Stanisław Karpiński |
| author_sort | Maciej Jerzy Bernacki |
| collection | DOAJ |
| description | Production of biofuel from lignocellulosic biomass is relatively low due to the limited knowledge about natural cell wall loosening and cellulolytic processes in plants. Industrial separation of cellulose fiber mass from lignin, its saccharification and alcoholic fermentation is still cost-ineffective and environmentally unfriendly. Assuming that the green transformation is inevitable and that new sources of raw materials for biofuels are needed, we decided to study cell death—a natural process occurring in plants in the context of reducing the recalcitrance of lignocellulose for the production of second-generation bioethanol. “Members of the enzyme families responsible for lysigenous aerenchyma formation were identified during the root hypoxia stress in <i>Arabidopsis thaliana</i> cell death mutants. The cell death regulatory genes, LESION SIMULATING DISEASE 1 (LSD1), PHYTOALEXIN DEFICIENT 4 (PAD4) and ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) conditionally regulate the cell wall when suppressed in transgenic aspen. During four years of growth in the field, the following effects were observed: lignin content was reduced, the cellulose fiber polymerization degree increased and the growth itself was unaffected. The wood of transgenic trees was more efficient as a substrate for saccharification, alcoholic fermentation and bioethanol production. The presented results may trigger the development of novel biotechnologies in the biofuel industry. |
| first_indexed | 2024-03-11T00:03:40Z |
| format | Article |
| id | doaj.art-bb809871031241888e8907941d122c66 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T00:03:40Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-bb809871031241888e8907941d122c662023-11-19T00:36:10ZengMDPI AGCells2073-44092023-08-011216201810.3390/cells12162018Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel IndustryMaciej Jerzy Bernacki0Jakub Mielecki1Andrzej Antczak2Michał Drożdżek3Damian Witoń4Joanna Dąbrowska-Bronk5Piotr Gawroński6Paweł Burdiak7Monika Marchwicka8Anna Rusaczonek9Katarzyna Dąbkowska-Susfał10Wacław Roman Strobel11Ewa J. Mellerowicz12Janusz Zawadzki13Magdalena Szechyńska-Hebda14Stanisław Karpiński15Department of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warsaw, PolandInstitute of Wood Sciences and Furniture, Warsaw University of Life Sciences—SGGW, 02-776 Warsaw, PolandInstitute of Wood Sciences and Furniture, Warsaw University of Life Sciences—SGGW, 02-776 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warsaw, PolandDepartment of Plant Physiology, Institute of Biology, Warsaw University of Life Sciences, 02-776 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warsaw, PolandInstitute of Wood Sciences and Furniture, Warsaw University of Life Sciences—SGGW, 02-776 Warsaw, PolandDepartment of Botany, Institute of Biology, Warsaw University of Life Sciences, 02-776 Warsaw, PolandFaculty of Chemical and Process Engineering, Warsaw University of Technology, 00-645 Warsaw, PolandInstitute of Technology and Life Sciences—National Research Institute, Falenty, Al. Hrabska 3, 05-090 Raszyn, PolandDepartment of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901-83 Umeå, SwedenInstitute of Wood Sciences and Furniture, Warsaw University of Life Sciences—SGGW, 02-776 Warsaw, PolandW. Szafer Institute of Botany Polish Academy of Sciences, Lubicz 46, 31-512 Kraków, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warsaw, PolandProduction of biofuel from lignocellulosic biomass is relatively low due to the limited knowledge about natural cell wall loosening and cellulolytic processes in plants. Industrial separation of cellulose fiber mass from lignin, its saccharification and alcoholic fermentation is still cost-ineffective and environmentally unfriendly. Assuming that the green transformation is inevitable and that new sources of raw materials for biofuels are needed, we decided to study cell death—a natural process occurring in plants in the context of reducing the recalcitrance of lignocellulose for the production of second-generation bioethanol. “Members of the enzyme families responsible for lysigenous aerenchyma formation were identified during the root hypoxia stress in <i>Arabidopsis thaliana</i> cell death mutants. The cell death regulatory genes, LESION SIMULATING DISEASE 1 (LSD1), PHYTOALEXIN DEFICIENT 4 (PAD4) and ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) conditionally regulate the cell wall when suppressed in transgenic aspen. During four years of growth in the field, the following effects were observed: lignin content was reduced, the cellulose fiber polymerization degree increased and the growth itself was unaffected. The wood of transgenic trees was more efficient as a substrate for saccharification, alcoholic fermentation and bioethanol production. The presented results may trigger the development of novel biotechnologies in the biofuel industry.https://www.mdpi.com/2073-4409/12/16/2018poplarligninbioethanolcell wallscarificationcell death |
| spellingShingle | Maciej Jerzy Bernacki Jakub Mielecki Andrzej Antczak Michał Drożdżek Damian Witoń Joanna Dąbrowska-Bronk Piotr Gawroński Paweł Burdiak Monika Marchwicka Anna Rusaczonek Katarzyna Dąbkowska-Susfał Wacław Roman Strobel Ewa J. Mellerowicz Janusz Zawadzki Magdalena Szechyńska-Hebda Stanisław Karpiński Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry Cells poplar lignin bioethanol cell wall scarification cell death |
| title | Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry |
| title_full | Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry |
| title_fullStr | Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry |
| title_full_unstemmed | Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry |
| title_short | Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry |
| title_sort | biotechnological potential of the stress response and plant cell death regulators proteins in the biofuel industry |
| topic | poplar lignin bioethanol cell wall scarification cell death |
| url | https://www.mdpi.com/2073-4409/12/16/2018 |
| work_keys_str_mv | AT maciejjerzybernacki biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT jakubmielecki biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT andrzejantczak biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT michałdrozdzek biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT damianwiton biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT joannadabrowskabronk biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT piotrgawronski biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT pawełburdiak biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT monikamarchwicka biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT annarusaczonek biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT katarzynadabkowskasusfał biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT wacławromanstrobel biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT ewajmellerowicz biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT januszzawadzki biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT magdalenaszechynskahebda biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry AT stanisławkarpinski biotechnologicalpotentialofthestressresponseandplantcelldeathregulatorsproteinsinthebiofuelindustry |