Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae
Pegah Shakib1,2, Sobhan Ghafourian1, Mohammad Reza Zolfaghary2, Reza Hushmandfar3, Reza Ranjbar4, Nourkhoda Sadeghifard11Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam; 2Department of Microbiology, Qom Branch, Islamic Azad University of Qom, Qom; 3Faculty of Veterin...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2011-12-01
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| Series: | Biologics: Targets & Therapy |
| Online Access: | http://www.dovepress.com/prevalence-of-ompk35-and-ompk36-porin-expression-in-beta-lactamase-and-a8943 |
| _version_ | 1818754279364624384 |
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| author | Shakib P Ghafourian S Zolfaghary MR Hushmandfar R Ranjbar R Sadeghifard N |
| author_facet | Shakib P Ghafourian S Zolfaghary MR Hushmandfar R Ranjbar R Sadeghifard N |
| author_sort | Shakib P |
| collection | DOAJ |
| description | Pegah Shakib1,2, Sobhan Ghafourian1, Mohammad Reza Zolfaghary2, Reza Hushmandfar3, Reza Ranjbar4, Nourkhoda Sadeghifard11Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam; 2Department of Microbiology, Qom Branch, Islamic Azad University of Qom, Qom; 3Faculty of Veterinary Medicine, Ilam University, Ilam; 4Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranBackground: The aims of this study were to confirm the presence of OmpK35 and OmpK36 in extended-spectrum beta-lactamase-producing and nonextended-spectrum beta-lactamase-producing Klebsiella pneumoniae and to determine the relationship between porin expression and resistance to third-generation cephalosporins.Methods: Fifty-two K. pneumoniae isolates were obtained and analyzed for extended-spectrum beta-lactamase and for OmpK35 and OmpK36.Results: Twenty-two (42.3%) isolates of K. pneumoniae were extended-spectrum beta-lactamase producers. The OmpK35 profile in K. pneumoniae producing extended-spectrum beta-lactamase showed the presence of porin protein in ceftazidime-sensitive K. pneumoniae (six isolates), and the OmpK36 profile in K. pneumoniae producing extended-spectrum beta-lactamase revealed isolates sensitive to cefotaxime (n = 8) and ceftriaxone (n = 6). All nonextended-spectrum beta-lactamase-producing K. pneumoniae showed the presence of OmpK35 and OmpK36 porin proteins.Conclusion: The presence of OmpK35 is mostly related to ceftazidime susceptibility and less to cefotaxime and ceftriaxone susceptibility, while OmpK36 expression is seen more often in cefotaxime-sensitive isolates. OmpK35 and OmpK36 indicate nonextended-spectrum beta-lactamase producing strains, and their presence is important when selecting an antimicrobial agent.Keywords: Klebsiella pneumoniae, extended-spectrum beta-lactamase producing strains, porin expression |
| first_indexed | 2024-12-18T05:20:44Z |
| format | Article |
| id | doaj.art-bb83c984b56f4537a27034a7b04b629c |
| institution | Directory Open Access Journal |
| issn | 1177-5475 1177-5491 |
| language | English |
| last_indexed | 2024-12-18T05:20:44Z |
| publishDate | 2011-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Biologics: Targets & Therapy |
| spelling | doaj.art-bb83c984b56f4537a27034a7b04b629c2022-12-21T21:19:40ZengDove Medical PressBiologics: Targets & Therapy1177-54751177-54912011-12-012012default14Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniaeShakib PGhafourian SZolfaghary MRHushmandfar RRanjbar RSadeghifard NPegah Shakib1,2, Sobhan Ghafourian1, Mohammad Reza Zolfaghary2, Reza Hushmandfar3, Reza Ranjbar4, Nourkhoda Sadeghifard11Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam; 2Department of Microbiology, Qom Branch, Islamic Azad University of Qom, Qom; 3Faculty of Veterinary Medicine, Ilam University, Ilam; 4Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranBackground: The aims of this study were to confirm the presence of OmpK35 and OmpK36 in extended-spectrum beta-lactamase-producing and nonextended-spectrum beta-lactamase-producing Klebsiella pneumoniae and to determine the relationship between porin expression and resistance to third-generation cephalosporins.Methods: Fifty-two K. pneumoniae isolates were obtained and analyzed for extended-spectrum beta-lactamase and for OmpK35 and OmpK36.Results: Twenty-two (42.3%) isolates of K. pneumoniae were extended-spectrum beta-lactamase producers. The OmpK35 profile in K. pneumoniae producing extended-spectrum beta-lactamase showed the presence of porin protein in ceftazidime-sensitive K. pneumoniae (six isolates), and the OmpK36 profile in K. pneumoniae producing extended-spectrum beta-lactamase revealed isolates sensitive to cefotaxime (n = 8) and ceftriaxone (n = 6). All nonextended-spectrum beta-lactamase-producing K. pneumoniae showed the presence of OmpK35 and OmpK36 porin proteins.Conclusion: The presence of OmpK35 is mostly related to ceftazidime susceptibility and less to cefotaxime and ceftriaxone susceptibility, while OmpK36 expression is seen more often in cefotaxime-sensitive isolates. OmpK35 and OmpK36 indicate nonextended-spectrum beta-lactamase producing strains, and their presence is important when selecting an antimicrobial agent.Keywords: Klebsiella pneumoniae, extended-spectrum beta-lactamase producing strains, porin expressionhttp://www.dovepress.com/prevalence-of-ompk35-and-ompk36-porin-expression-in-beta-lactamase-and-a8943 |
| spellingShingle | Shakib P Ghafourian S Zolfaghary MR Hushmandfar R Ranjbar R Sadeghifard N Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae Biologics: Targets & Therapy |
| title | Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae |
| title_full | Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae |
| title_fullStr | Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae |
| title_full_unstemmed | Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae |
| title_short | Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae |
| title_sort | prevalence of ompk35 and ompk36 porin expression in beta lactamase and non beta lactamase producing klebsiella pneumoniae |
| url | http://www.dovepress.com/prevalence-of-ompk35-and-ompk36-porin-expression-in-beta-lactamase-and-a8943 |
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