Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde

A new simple, accurate and cost-effective spectrophotometric method has been developed for the analysis of some cephalosporins (ceftriaxone, ceftazidime, cefixime, cefotaxime and cefuroxime) in bulk samples and pharmaceutical dosage forms. The reaction involves a two-step process of diazotization of...

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Main Authors: Olajire A. Adegoke, Monsurat O. Quadri
Format: Article
Language:English
Published: Elsevier 2016-11-01
Series:Arabian Journal of Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1878535212000329
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author Olajire A. Adegoke
Monsurat O. Quadri
author_facet Olajire A. Adegoke
Monsurat O. Quadri
author_sort Olajire A. Adegoke
collection DOAJ
description A new simple, accurate and cost-effective spectrophotometric method has been developed for the analysis of some cephalosporins (ceftriaxone, ceftazidime, cefixime, cefotaxime and cefuroxime) in bulk samples and pharmaceutical dosage forms. The reaction involves a two-step process of diazotization of the cephalosporins with acidified NaNO2 at 0–5 °C and coupling with acidified p-dimethylaminobenzaldehyde (DMAB). Optimal temperature and time for coupling were established at 50 and 60 °C with coupling time ranging from 15 to 20 min. All the cephalosporins gave azo adducts with DMAB that absorbed light optimally at 400–430 nm. The reaction with DMAB occurred at a stoichiometric ratio of 1:1. Optimization of DMAB concentration revealed the superiority of using 0.3% DMAB in 0.0625 M H2SO4 with the best diluting solvent being methanol. Beer’s law was obeyed at concentrations ranging from 5 to 60 μg/mL with correlation coefficients >0.9980 in all cases. Overall recoveries were of the order of 95–103% with errors generally less than 6%. The method was successfully applied to the determination of the cephalosporins in dosage forms and it was found to be equivalent accuracy to the official (USP and BP) HPLC assay procedures for these drugs. There was no interference from commonly adopted excipients. The method could find application as a rapid and cost-effective alternative for the quality control of these cephalosporins, especially in preliminary studies.
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spelling doaj.art-bb84290ccdd34531806598f406d9521c2022-12-21T19:01:31ZengElsevierArabian Journal of Chemistry1878-53522016-11-019S2S1272S128210.1016/j.arabjc.2012.02.005Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehydeOlajire A. AdegokeMonsurat O. QuadriA new simple, accurate and cost-effective spectrophotometric method has been developed for the analysis of some cephalosporins (ceftriaxone, ceftazidime, cefixime, cefotaxime and cefuroxime) in bulk samples and pharmaceutical dosage forms. The reaction involves a two-step process of diazotization of the cephalosporins with acidified NaNO2 at 0–5 °C and coupling with acidified p-dimethylaminobenzaldehyde (DMAB). Optimal temperature and time for coupling were established at 50 and 60 °C with coupling time ranging from 15 to 20 min. All the cephalosporins gave azo adducts with DMAB that absorbed light optimally at 400–430 nm. The reaction with DMAB occurred at a stoichiometric ratio of 1:1. Optimization of DMAB concentration revealed the superiority of using 0.3% DMAB in 0.0625 M H2SO4 with the best diluting solvent being methanol. Beer’s law was obeyed at concentrations ranging from 5 to 60 μg/mL with correlation coefficients >0.9980 in all cases. Overall recoveries were of the order of 95–103% with errors generally less than 6%. The method was successfully applied to the determination of the cephalosporins in dosage forms and it was found to be equivalent accuracy to the official (USP and BP) HPLC assay procedures for these drugs. There was no interference from commonly adopted excipients. The method could find application as a rapid and cost-effective alternative for the quality control of these cephalosporins, especially in preliminary studies.http://www.sciencedirect.com/science/article/pii/S1878535212000329CephalosporinsDiazotizationp-DimethylaminobenzaldehydeDiazo coupling reactionSpectrophotometric analysis
spellingShingle Olajire A. Adegoke
Monsurat O. Quadri
Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde
Arabian Journal of Chemistry
Cephalosporins
Diazotization
p-Dimethylaminobenzaldehyde
Diazo coupling reaction
Spectrophotometric analysis
title Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde
title_full Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde
title_fullStr Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde
title_full_unstemmed Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde
title_short Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde
title_sort novel spectrophotometric determinations of some cephalosporins following azo dye formation with p dimethylaminobenzaldehyde
topic Cephalosporins
Diazotization
p-Dimethylaminobenzaldehyde
Diazo coupling reaction
Spectrophotometric analysis
url http://www.sciencedirect.com/science/article/pii/S1878535212000329
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