Chemokine-Decorated Nanoparticles Target Specific Subpopulations of Primary Blood Mononuclear Leukocytes
Specific cell targeting to deliver nanoparticles can be achieved by tailored modifications of the material surface with chemical moieties. The selection of the cell targets can be optimized by covering the nanoparticle with molecules, the receptor expression of which is restricted to particular cell...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/20/3560 |
| _version_ | 1797470775923441664 |
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| author | Anissa Pisani Roberto Donno Giulio Valenti Pier Paolo Pompa Nicola Tirelli Giuseppe Bardi |
| author_facet | Anissa Pisani Roberto Donno Giulio Valenti Pier Paolo Pompa Nicola Tirelli Giuseppe Bardi |
| author_sort | Anissa Pisani |
| collection | DOAJ |
| description | Specific cell targeting to deliver nanoparticles can be achieved by tailored modifications of the material surface with chemical moieties. The selection of the cell targets can be optimized by covering the nanoparticle with molecules, the receptor expression of which is restricted to particular cell subsets. Chemokines perform their biological action through 7-TM G<sub>i</sub>-protein-coupled receptors differently expressed in all tissues. We decorated the surface of biocompatible polymer nanoparticles with full-length CCL5, an inflammatory chemokine that attracts leukocytes by binding CCR5, which is highly expressed in blood-circulating monocytes. Our observations showed that CCL5 functionalization does not affect the nanoparticle biocompatibility. Notably, CCL5 NPs delivered to PBMCs are selectively internalized by CCR5<sup>+</sup> monocytes but not by CCR5<sup>-</sup> lymphocytes. The efficacy of PBMC subpopulation targeting by chemokine-decorated nanoparticles establishes an easy-to-use functionalization for specific leukocyte delivery. |
| first_indexed | 2024-03-09T19:40:45Z |
| format | Article |
| id | doaj.art-bb8721f734214c39ade09aacf2a8a7f1 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T19:40:45Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-bb8721f734214c39ade09aacf2a8a7f12023-11-24T01:39:11ZengMDPI AGNanomaterials2079-49912022-10-011220356010.3390/nano12203560Chemokine-Decorated Nanoparticles Target Specific Subpopulations of Primary Blood Mononuclear LeukocytesAnissa Pisani0Roberto Donno1Giulio Valenti2Pier Paolo Pompa3Nicola Tirelli4Giuseppe Bardi5Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, ItalyLaboratory of Polymers and Biomaterials, Istituto Italiano di Tecnologia, 16163 Genova, ItalyLaboratory of Polymers and Biomaterials, Istituto Italiano di Tecnologia, 16163 Genova, ItalyNanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, ItalyLaboratory of Polymers and Biomaterials, Istituto Italiano di Tecnologia, 16163 Genova, ItalyNanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, ItalySpecific cell targeting to deliver nanoparticles can be achieved by tailored modifications of the material surface with chemical moieties. The selection of the cell targets can be optimized by covering the nanoparticle with molecules, the receptor expression of which is restricted to particular cell subsets. Chemokines perform their biological action through 7-TM G<sub>i</sub>-protein-coupled receptors differently expressed in all tissues. We decorated the surface of biocompatible polymer nanoparticles with full-length CCL5, an inflammatory chemokine that attracts leukocytes by binding CCR5, which is highly expressed in blood-circulating monocytes. Our observations showed that CCL5 functionalization does not affect the nanoparticle biocompatibility. Notably, CCL5 NPs delivered to PBMCs are selectively internalized by CCR5<sup>+</sup> monocytes but not by CCR5<sup>-</sup> lymphocytes. The efficacy of PBMC subpopulation targeting by chemokine-decorated nanoparticles establishes an easy-to-use functionalization for specific leukocyte delivery.https://www.mdpi.com/2079-4991/12/20/3560nanoparticleschemokinetargetingPBMCs |
| spellingShingle | Anissa Pisani Roberto Donno Giulio Valenti Pier Paolo Pompa Nicola Tirelli Giuseppe Bardi Chemokine-Decorated Nanoparticles Target Specific Subpopulations of Primary Blood Mononuclear Leukocytes Nanomaterials nanoparticles chemokine targeting PBMCs |
| title | Chemokine-Decorated Nanoparticles Target Specific Subpopulations of Primary Blood Mononuclear Leukocytes |
| title_full | Chemokine-Decorated Nanoparticles Target Specific Subpopulations of Primary Blood Mononuclear Leukocytes |
| title_fullStr | Chemokine-Decorated Nanoparticles Target Specific Subpopulations of Primary Blood Mononuclear Leukocytes |
| title_full_unstemmed | Chemokine-Decorated Nanoparticles Target Specific Subpopulations of Primary Blood Mononuclear Leukocytes |
| title_short | Chemokine-Decorated Nanoparticles Target Specific Subpopulations of Primary Blood Mononuclear Leukocytes |
| title_sort | chemokine decorated nanoparticles target specific subpopulations of primary blood mononuclear leukocytes |
| topic | nanoparticles chemokine targeting PBMCs |
| url | https://www.mdpi.com/2079-4991/12/20/3560 |
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