Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure
The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-12-01
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| Series: | EuPA Open Proteomics |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212968515300192 |
| _version_ | 1818312612295737344 |
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| author | Gregory W. Watson Samanthi Wickramasekara Claudia S. Maier David E. Williams Roderick H. Dashwood Emily Ho |
| author_facet | Gregory W. Watson Samanthi Wickramasekara Claudia S. Maier David E. Williams Roderick H. Dashwood Emily Ho |
| author_sort | Gregory W. Watson |
| collection | DOAJ |
| description | The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC–MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC–MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells. Keywords: Prostate cancer, Sulforaphane, Mass spectrometry |
| first_indexed | 2024-12-13T08:20:37Z |
| format | Article |
| id | doaj.art-bb8a4ed7f7f3414ea4c47adfc1f22b14 |
| institution | Directory Open Access Journal |
| issn | 2212-9685 |
| language | English |
| last_indexed | 2024-12-13T08:20:37Z |
| publishDate | 2015-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EuPA Open Proteomics |
| spelling | doaj.art-bb8a4ed7f7f3414ea4c47adfc1f22b142022-12-21T23:54:02ZengElsevierEuPA Open Proteomics2212-96852015-12-0193440Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposureGregory W. Watson0Samanthi Wickramasekara1Claudia S. Maier2David E. Williams3Roderick H. Dashwood4Emily Ho5Molecular and Cellular Biology, Oregon State University, Corvallis, OR, United States; Biological and Population Health Sciences, Oregon State University, Corvallis, OR, United StatesDepartment of Chemistry, Oregon State University, Corvallis, OR, United StatesDepartment of Chemistry, Oregon State University, Corvallis, OR, United StatesEnvironmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States; Linus Pauling Institute, Oregon State University, Corvallis, OR, United StatesCenter for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M Science Center, Houston, TX, United States; Department of Nutrition & Food Science, Texas A&M University, College Station, TX, United States; Department of Clinical Cancer Prevention, MD Anderson Cancer Center, Houston, TX, United States; Department of Molecular & Cellular Medicine, Texas A&M University College of Medicine, College Station, TX, United StatesBiological and Population Health Sciences, Oregon State University, Corvallis, OR, United States; Linus Pauling Institute, Oregon State University, Corvallis, OR, United States; Corresponding author at: School of Biological and Population Health Sciences, 103 Milam Hall, Oregon State University, Corvallis, OR 97331, United States. Fax: +541 737 6914.The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC–MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC–MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells. Keywords: Prostate cancer, Sulforaphane, Mass spectrometryhttp://www.sciencedirect.com/science/article/pii/S2212968515300192 |
| spellingShingle | Gregory W. Watson Samanthi Wickramasekara Claudia S. Maier David E. Williams Roderick H. Dashwood Emily Ho Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure EuPA Open Proteomics |
| title | Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure |
| title_full | Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure |
| title_fullStr | Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure |
| title_full_unstemmed | Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure |
| title_short | Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure |
| title_sort | assessment of global proteome in lncap cells by 2d rp rp lc ms ms following sulforaphane exposure |
| url | http://www.sciencedirect.com/science/article/pii/S2212968515300192 |
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