Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children
The present study aimed to determine the capsular serotype distribution and antimicrobial drug resistance patterns of Haemophilus influenzae from children in the Kunming region of China. This information could guide policymakers in clinical treatment. In the present study, H. influenzae isolates wer...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2023-06-01
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| Series: | Polish Journal of Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.33073/pjm-2023-006 |
| _version_ | 1797800796843147264 |
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| author | Yuan Mei Ma Mingbiao Jiang Hongchao Fan Mao Sun Ying Zhou Bailing Feng Xingxing Yang Junyi Su Min He Xiaoli |
| author_facet | Yuan Mei Ma Mingbiao Jiang Hongchao Fan Mao Sun Ying Zhou Bailing Feng Xingxing Yang Junyi Su Min He Xiaoli |
| author_sort | Yuan Mei |
| collection | DOAJ |
| description | The present study aimed to determine the capsular serotype distribution and antimicrobial drug resistance patterns of Haemophilus influenzae from children in the Kunming region of China. This information could guide policymakers in clinical treatment. In the present study, H. influenzae isolates were tested for their serotypes, antimicrobial susceptibility pattern, and presence of β-lactamases. One-hundred forty-eight H. influenzae strains isolated from children 0–2 years old were investigated for capsular types by glass slide agglutination and molecular methods, and biotyped by the biochemical reactions. The drug resistance-encoding genes TEM-1, ROB-1, and the ftsI gene mutations PBP3-3, and PBP3-BLN were detected with real-time quantitative polymerase chain reaction (qPCR). The prevalence of β-lactamase-producing strains (60.3%) was significantly higher (p < 0.05) than non-enzyme-producing strains. β-Lactamase-producing strains were multidrug resistant to various antibiotics such as ampicillin, tetracycline, sulfamethoxazole/trimethoprim, chloramphenicol, cefuroxime, and cefaclor. Among β-lactamase-producing strains, the detection rates of the TEM-1, PBP3-BLN, PBP3-s, and ROB-1 were 54.1%, 18.9%, 11.8%, and 6.9%, respectively. The biotyping results show that most H. influenzae strains were of type II and III. Non-typeable H. influenzae (NTHi) accounted for 89.3% of the strains. NTHi strains were the most prevalent in this region; most belonged to biological types II and III. β-Lactamase-positive ampi-cillin-resistant (BLPAR) strains were prevalent among H. influenzae isolates in this region. |
| first_indexed | 2024-03-13T04:40:40Z |
| format | Article |
| id | doaj.art-bb8d370c496942c5a081be5de313f35a |
| institution | Directory Open Access Journal |
| issn | 2544-4646 |
| language | English |
| last_indexed | 2024-03-13T04:40:40Z |
| publishDate | 2023-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-bb8d370c496942c5a081be5de313f35a2023-06-19T05:55:35ZengSciendoPolish Journal of Microbiology2544-46462023-06-0172212513110.33073/pjm-2023-006Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming ChildrenYuan Mei0Ma Mingbiao1Jiang Hongchao2Fan Mao3Sun Ying4Zhou Bailing5Feng Xingxing6Yang Junyi7Su Min8He Xiaoli91Department of Laboratory, Children’s Hospital affiliated with Kunming Medical University, Kunming, China1Department of Laboratory, Children’s Hospital affiliated with Kunming Medical University, Kunming, China2Science and Education Section, Children’s Hospital affiliated with Kunming Medical University, Kunming, China1Department of Laboratory, Children’s Hospital affiliated with Kunming Medical University, Kunming, China3Epilepsy Center of Children, Children’s Hospital affiliated with Kunming Medical University, Kunming, China1Department of Laboratory, Children’s Hospital affiliated with Kunming Medical University, Kunming, China1Department of Laboratory, Children’s Hospital affiliated with Kunming Medical University, Kunming, China1Department of Laboratory, Children’s Hospital affiliated with Kunming Medical University, Kunming, China1Department of Laboratory, Children’s Hospital affiliated with Kunming Medical University, Kunming, China4Institute of Pediatrics, Children’s Hospital affiliated with Kunming Medical University, Kunming, ChinaThe present study aimed to determine the capsular serotype distribution and antimicrobial drug resistance patterns of Haemophilus influenzae from children in the Kunming region of China. This information could guide policymakers in clinical treatment. In the present study, H. influenzae isolates were tested for their serotypes, antimicrobial susceptibility pattern, and presence of β-lactamases. One-hundred forty-eight H. influenzae strains isolated from children 0–2 years old were investigated for capsular types by glass slide agglutination and molecular methods, and biotyped by the biochemical reactions. The drug resistance-encoding genes TEM-1, ROB-1, and the ftsI gene mutations PBP3-3, and PBP3-BLN were detected with real-time quantitative polymerase chain reaction (qPCR). The prevalence of β-lactamase-producing strains (60.3%) was significantly higher (p < 0.05) than non-enzyme-producing strains. β-Lactamase-producing strains were multidrug resistant to various antibiotics such as ampicillin, tetracycline, sulfamethoxazole/trimethoprim, chloramphenicol, cefuroxime, and cefaclor. Among β-lactamase-producing strains, the detection rates of the TEM-1, PBP3-BLN, PBP3-s, and ROB-1 were 54.1%, 18.9%, 11.8%, and 6.9%, respectively. The biotyping results show that most H. influenzae strains were of type II and III. Non-typeable H. influenzae (NTHi) accounted for 89.3% of the strains. NTHi strains were the most prevalent in this region; most belonged to biological types II and III. β-Lactamase-positive ampi-cillin-resistant (BLPAR) strains were prevalent among H. influenzae isolates in this region.https://doi.org/10.33073/pjm-2023-006emophilus influenzaebiotyping and capsule typingresistant phenotype |
| spellingShingle | Yuan Mei Ma Mingbiao Jiang Hongchao Fan Mao Sun Ying Zhou Bailing Feng Xingxing Yang Junyi Su Min He Xiaoli Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children Polish Journal of Microbiology emophilus influenzae biotyping and capsule typing resistant phenotype |
| title | Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children |
| title_full | Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children |
| title_fullStr | Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children |
| title_full_unstemmed | Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children |
| title_short | Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children |
| title_sort | characterization of serotypes and molecular drug resistance patterns of haemophilus influenzae in kunming children |
| topic | emophilus influenzae biotyping and capsule typing resistant phenotype |
| url | https://doi.org/10.33073/pjm-2023-006 |
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