Silencing the <i>CsSnRK2.11</i> Gene Decreases Drought Tolerance of <i>Cucumis sativus</i> L.
Drought stress restricts vegetable growth, and abscisic acid plays an important role in its regulation. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses....
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15761 |
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| author | Peng Wang Zilong Wan Shilei Luo Haotai Wei Jianuo Zhao Guoshuai Wang Jihua Yu Guobin Zhang |
| author_facet | Peng Wang Zilong Wan Shilei Luo Haotai Wei Jianuo Zhao Guoshuai Wang Jihua Yu Guobin Zhang |
| author_sort | Peng Wang |
| collection | DOAJ |
| description | Drought stress restricts vegetable growth, and abscisic acid plays an important role in its regulation. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. Our previous experiments demonstrated that the <i>SnRK2.11</i> gene exhibits a significant response to drought stress in cucumbers. To further investigate the function of <i>SnRK2.11</i> under drought stress, we used VIGS (virus-induced gene silencing) technology to silence this gene and conducted RNA-seq analysis. The <i>SnRK2.11</i>-silencing plants displayed increased sensitivity to drought stress, which led to stunted growth and increased wilting speed. Moreover, various physiological parameters related to photosynthesis, chlorophyll fluorescence, leaf water content, chlorophyll content, and antioxidant enzyme activity were significantly reduced. The intercellular CO<sub>2</sub> concentration, non-photochemical burst coefficient, and malondialdehyde and proline content were significantly increased. RNA-seq analysis identified 534 differentially expressed genes (DEGs): 311 were upregulated and 223 were downregulated. GO functional annotation analysis indicated that these DEGs were significantly enriched for molecular functions related to host cells, enzyme activity, and stress responses. KEGG pathway enrichment analysis further revealed that these DEGs were significantly enriched in phytohormone signalling, MAPK signalling, and carotenoid biosynthesis pathways, all of which were associated with abscisic acid. This study used VIGS technology and transcriptome data to investigate the role of <i>CsSnRK2.11</i> under drought stress, offering valuable insights into the mechanism of the SnRK2 gene in enhancing drought resistance in cucumbers. |
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| language | English |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bb8f36626d51420a84f2371a5e2c89b32023-11-10T15:05:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211576110.3390/ijms242115761Silencing the <i>CsSnRK2.11</i> Gene Decreases Drought Tolerance of <i>Cucumis sativus</i> L.Peng Wang0Zilong Wan1Shilei Luo2Haotai Wei3Jianuo Zhao4Guoshuai Wang5Jihua Yu6Guobin Zhang7State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaDrought stress restricts vegetable growth, and abscisic acid plays an important role in its regulation. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. Our previous experiments demonstrated that the <i>SnRK2.11</i> gene exhibits a significant response to drought stress in cucumbers. To further investigate the function of <i>SnRK2.11</i> under drought stress, we used VIGS (virus-induced gene silencing) technology to silence this gene and conducted RNA-seq analysis. The <i>SnRK2.11</i>-silencing plants displayed increased sensitivity to drought stress, which led to stunted growth and increased wilting speed. Moreover, various physiological parameters related to photosynthesis, chlorophyll fluorescence, leaf water content, chlorophyll content, and antioxidant enzyme activity were significantly reduced. The intercellular CO<sub>2</sub> concentration, non-photochemical burst coefficient, and malondialdehyde and proline content were significantly increased. RNA-seq analysis identified 534 differentially expressed genes (DEGs): 311 were upregulated and 223 were downregulated. GO functional annotation analysis indicated that these DEGs were significantly enriched for molecular functions related to host cells, enzyme activity, and stress responses. KEGG pathway enrichment analysis further revealed that these DEGs were significantly enriched in phytohormone signalling, MAPK signalling, and carotenoid biosynthesis pathways, all of which were associated with abscisic acid. This study used VIGS technology and transcriptome data to investigate the role of <i>CsSnRK2.11</i> under drought stress, offering valuable insights into the mechanism of the SnRK2 gene in enhancing drought resistance in cucumbers.https://www.mdpi.com/1422-0067/24/21/15761VIGSdrought stressphotosynthesisantioxidanttranscriptome |
| spellingShingle | Peng Wang Zilong Wan Shilei Luo Haotai Wei Jianuo Zhao Guoshuai Wang Jihua Yu Guobin Zhang Silencing the <i>CsSnRK2.11</i> Gene Decreases Drought Tolerance of <i>Cucumis sativus</i> L. International Journal of Molecular Sciences VIGS drought stress photosynthesis antioxidant transcriptome |
| title | Silencing the <i>CsSnRK2.11</i> Gene Decreases Drought Tolerance of <i>Cucumis sativus</i> L. |
| title_full | Silencing the <i>CsSnRK2.11</i> Gene Decreases Drought Tolerance of <i>Cucumis sativus</i> L. |
| title_fullStr | Silencing the <i>CsSnRK2.11</i> Gene Decreases Drought Tolerance of <i>Cucumis sativus</i> L. |
| title_full_unstemmed | Silencing the <i>CsSnRK2.11</i> Gene Decreases Drought Tolerance of <i>Cucumis sativus</i> L. |
| title_short | Silencing the <i>CsSnRK2.11</i> Gene Decreases Drought Tolerance of <i>Cucumis sativus</i> L. |
| title_sort | silencing the i cssnrk2 11 i gene decreases drought tolerance of i cucumis sativus i l |
| topic | VIGS drought stress photosynthesis antioxidant transcriptome |
| url | https://www.mdpi.com/1422-0067/24/21/15761 |
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