New disease on red flowering horse chestnut (<em>Aesculus</em> x <em>carnea</em> Hayne) and the molecular identification of the involved pathogens
A so far unknown disease which is found on red flowering horse chestnut (Aesculus × carnea Hayne) in the north-west of Germany has been investigated. It is characterized by the presence of fruiting bodies of Flammulina velutipes (Curtis) Singer and Pleurotus ostreatus (Jacq. ex Fr.) Kummer, able to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | deu |
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Julius Kühn-Institut
2014-12-01
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| Series: | Journal für Kulturpflanzen |
| Subjects: | |
| Online Access: | https://ojs.openagrar.de/index.php/Kulturpflanzenjournal/article/view/12561 |
| _version_ | 1818791752093401088 |
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| author | Annika Müller-Navarra Oliver Gaiser Ute Moreth Dirk Dujesiefken Elisabeth Annemarie Magel |
| author_facet | Annika Müller-Navarra Oliver Gaiser Ute Moreth Dirk Dujesiefken Elisabeth Annemarie Magel |
| author_sort | Annika Müller-Navarra |
| collection | DOAJ |
| description | A so far unknown disease which is found on red flowering horse chestnut (Aesculus × carnea Hayne) in the north-west of Germany has been investigated. It is characterized by the presence of fruiting bodies of Flammulina velutipes (Curtis) Singer and Pleurotus ostreatus (Jacq. ex Fr.) Kummer, able to cause breakage of branches and stems by white rot. Additional infection by Pseudomonas syringae pv. aesculi, and cracks of the bark are found.
To get detailed knowledge of the disease, samples were taken from cross sections of four decayed red flowering horse chestnut stems. The investigation of individual tissues across the stem enables the assignment of the pathogens to the new disease. Sequencing of the rDNA-ITS identified F. velutipes and P. ostreatus, Bjerkandera adusta (Willd. ex Fr.) Karst. and Chondrostereum purpureum (Pers. ex Fr.) Pouzar, four white rot fungi, as main pathogens and some ascomycetes in the infected and decaying stem tissues.
Our studies revealed the co-appearance of different basidiomycetes and ascomycetes in decaying tissue zones which emphasizes the complexity of the disease. It is also the first proof of involvement of F. velutipes as a parasite on red flowering horse chestnut. In addition, for the first time, Pseudomonas syringae pv. aesculi was detected in the inner bark and within the secondary xylem of natural infected red flowering horse chestnut.
DOI: 10.5073/JfK.2014.12.03, https://doi.org/10.5073/JfK.2014.12.03 |
| first_indexed | 2024-12-18T15:16:20Z |
| format | Article |
| id | doaj.art-bb930db49e1e41ca8d79a0969af7b0be |
| institution | Directory Open Access Journal |
| issn | 1867-0911 1867-0938 |
| language | deu |
| last_indexed | 2024-12-18T15:16:20Z |
| publishDate | 2014-12-01 |
| publisher | Julius Kühn-Institut |
| record_format | Article |
| series | Journal für Kulturpflanzen |
| spelling | doaj.art-bb930db49e1e41ca8d79a0969af7b0be2022-12-21T21:03:30ZdeuJulius Kühn-InstitutJournal für Kulturpflanzen1867-09111867-09382014-12-016612New disease on red flowering horse chestnut (<em>Aesculus</em> x <em>carnea</em> Hayne) and the molecular identification of the involved pathogensAnnika Müller-Navarra0Oliver Gaiser1Ute Moreth2Dirk Dujesiefken3Elisabeth Annemarie Magel4Universität Hamburg, Zentrum Holzwirtschaft, Abteilung Holzbiologie und Holzschutz, HamburgInstitut für Baumpflege, HamburgUniversität Hamburg, Zentrum Holzwirtschaft, Abteilung Holzbiologie und Holzschutz, HamburgInstitut für Baumpflege, HamburgUniversität Hamburg, Zentrum Holzwirtschaft, Abteilung Holzbiologie und Holzschutz, HamburgA so far unknown disease which is found on red flowering horse chestnut (Aesculus × carnea Hayne) in the north-west of Germany has been investigated. It is characterized by the presence of fruiting bodies of Flammulina velutipes (Curtis) Singer and Pleurotus ostreatus (Jacq. ex Fr.) Kummer, able to cause breakage of branches and stems by white rot. Additional infection by Pseudomonas syringae pv. aesculi, and cracks of the bark are found. To get detailed knowledge of the disease, samples were taken from cross sections of four decayed red flowering horse chestnut stems. The investigation of individual tissues across the stem enables the assignment of the pathogens to the new disease. Sequencing of the rDNA-ITS identified F. velutipes and P. ostreatus, Bjerkandera adusta (Willd. ex Fr.) Karst. and Chondrostereum purpureum (Pers. ex Fr.) Pouzar, four white rot fungi, as main pathogens and some ascomycetes in the infected and decaying stem tissues. Our studies revealed the co-appearance of different basidiomycetes and ascomycetes in decaying tissue zones which emphasizes the complexity of the disease. It is also the first proof of involvement of F. velutipes as a parasite on red flowering horse chestnut. In addition, for the first time, Pseudomonas syringae pv. aesculi was detected in the inner bark and within the secondary xylem of natural infected red flowering horse chestnut. DOI: 10.5073/JfK.2014.12.03, https://doi.org/10.5073/JfK.2014.12.03https://ojs.openagrar.de/index.php/Kulturpflanzenjournal/article/view/12561Aesculus × carneanew diseasePseudomonas syringae pv. aesculiFlammulina velutipesPleurotus ostreatusBjerkandera adusta |
| spellingShingle | Annika Müller-Navarra Oliver Gaiser Ute Moreth Dirk Dujesiefken Elisabeth Annemarie Magel New disease on red flowering horse chestnut (<em>Aesculus</em> x <em>carnea</em> Hayne) and the molecular identification of the involved pathogens Journal für Kulturpflanzen Aesculus × carnea new disease Pseudomonas syringae pv. aesculi Flammulina velutipes Pleurotus ostreatus Bjerkandera adusta |
| title | New disease on red flowering horse chestnut (<em>Aesculus</em> x <em>carnea</em> Hayne) and the molecular identification of the involved pathogens |
| title_full | New disease on red flowering horse chestnut (<em>Aesculus</em> x <em>carnea</em> Hayne) and the molecular identification of the involved pathogens |
| title_fullStr | New disease on red flowering horse chestnut (<em>Aesculus</em> x <em>carnea</em> Hayne) and the molecular identification of the involved pathogens |
| title_full_unstemmed | New disease on red flowering horse chestnut (<em>Aesculus</em> x <em>carnea</em> Hayne) and the molecular identification of the involved pathogens |
| title_short | New disease on red flowering horse chestnut (<em>Aesculus</em> x <em>carnea</em> Hayne) and the molecular identification of the involved pathogens |
| title_sort | new disease on red flowering horse chestnut em aesculus em x em carnea em hayne and the molecular identification of the involved pathogens |
| topic | Aesculus × carnea new disease Pseudomonas syringae pv. aesculi Flammulina velutipes Pleurotus ostreatus Bjerkandera adusta |
| url | https://ojs.openagrar.de/index.php/Kulturpflanzenjournal/article/view/12561 |
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