The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic
Heterochromatin is enriched for specific epigenetic factors including Heterochromatin Protein 1a (HP1a), and is essential for many organismal functions. To elucidate heterochromatin organization and regulation, we purified Drosophila melanogaster HP1a interactors, and performed a genome-wide RNAi sc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-08-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/16096 |
| _version_ | 1811180635833761792 |
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| author | Joel M Swenson Serafin U Colmenares Amy R Strom Sylvain V Costes Gary H Karpen |
| author_facet | Joel M Swenson Serafin U Colmenares Amy R Strom Sylvain V Costes Gary H Karpen |
| author_sort | Joel M Swenson |
| collection | DOAJ |
| description | Heterochromatin is enriched for specific epigenetic factors including Heterochromatin Protein 1a (HP1a), and is essential for many organismal functions. To elucidate heterochromatin organization and regulation, we purified Drosophila melanogaster HP1a interactors, and performed a genome-wide RNAi screen to identify genes that impact HP1a levels or localization. The majority of the over four hundred putative HP1a interactors and regulators identified were previously unknown. We found that 13 of 16 tested candidates (83%) are required for gene silencing, providing a substantial increase in the number of identified components that impact heterochromatin properties. Surprisingly, image analysis revealed that although some HP1a interactors and regulators are broadly distributed within the heterochromatin domain, most localize to discrete subdomains that display dynamic localization patterns during the cell cycle. We conclude that heterochromatin composition and architecture is more spatially complex and dynamic than previously suggested, and propose that a network of subdomains regulates diverse heterochromatin functions. |
| first_indexed | 2024-04-11T09:06:29Z |
| format | Article |
| id | doaj.art-bb96ee37e16d40cf984cd84d1198df3e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:06:29Z |
| publishDate | 2016-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-bb96ee37e16d40cf984cd84d1198df3e2022-12-22T04:32:37ZengeLife Sciences Publications LtdeLife2050-084X2016-08-01510.7554/eLife.16096The composition and organization of Drosophila heterochromatin are heterogeneous and dynamicJoel M Swenson0Serafin U Colmenares1https://orcid.org/0000-0002-4094-4220Amy R Strom2Sylvain V Costes3Gary H Karpen4https://orcid.org/0000-0003-1534-0385Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, United StatesDivision of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, United StatesDivision of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, United States; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDivision of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, United StatesDivision of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, United States; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesHeterochromatin is enriched for specific epigenetic factors including Heterochromatin Protein 1a (HP1a), and is essential for many organismal functions. To elucidate heterochromatin organization and regulation, we purified Drosophila melanogaster HP1a interactors, and performed a genome-wide RNAi screen to identify genes that impact HP1a levels or localization. The majority of the over four hundred putative HP1a interactors and regulators identified were previously unknown. We found that 13 of 16 tested candidates (83%) are required for gene silencing, providing a substantial increase in the number of identified components that impact heterochromatin properties. Surprisingly, image analysis revealed that although some HP1a interactors and regulators are broadly distributed within the heterochromatin domain, most localize to discrete subdomains that display dynamic localization patterns during the cell cycle. We conclude that heterochromatin composition and architecture is more spatially complex and dynamic than previously suggested, and propose that a network of subdomains regulates diverse heterochromatin functions.https://elifesciences.org/articles/16096heterochromatincell biologygenome-wide RNAi screennuclear organizationposition effect variegation |
| spellingShingle | Joel M Swenson Serafin U Colmenares Amy R Strom Sylvain V Costes Gary H Karpen The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic eLife heterochromatin cell biology genome-wide RNAi screen nuclear organization position effect variegation |
| title | The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic |
| title_full | The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic |
| title_fullStr | The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic |
| title_full_unstemmed | The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic |
| title_short | The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic |
| title_sort | composition and organization of drosophila heterochromatin are heterogeneous and dynamic |
| topic | heterochromatin cell biology genome-wide RNAi screen nuclear organization position effect variegation |
| url | https://elifesciences.org/articles/16096 |
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