Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of Iran
Staphylococcus aureus is a common pathogen potentially able to cause a wide range of infectious diseases in human and animals and coagulase enzyme is one of the important virulence factors of this bacterium. Polymorphism of the coagulase encoding gene (coa) is one of the molecular-based typing metho...
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2018-09-01
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| Series: | Journal of Cell and Molecular Research |
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| Online Access: | https://jcmr.um.ac.ir/article_29408_24d0460f48fd6f8a16f646661ef7db8b.pdf |
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| author | Mohammad Reza Izadpanah Leila Asadpour |
| author_facet | Mohammad Reza Izadpanah Leila Asadpour |
| author_sort | Mohammad Reza Izadpanah |
| collection | DOAJ |
| description | Staphylococcus aureus is a common pathogen potentially able to cause a wide range of infectious diseases in human and animals and coagulase enzyme is one of the important virulence factors of this bacterium. Polymorphism of the coagulase encoding gene (coa) is one of the molecular-based typing methods of S. aureus isolates. In this study, the polymorphism of the coagulase gene among MRSA and MSSA isolates were investigated using PCR-RFLP analysis. To perform coagulase gene typing, the repeated units encoding hypervariable regions of coagulase gene of 30 clinical isolates of S. aureus were amplified by the PCR technique; this was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. In total two amplicons (680 bp and 750 bp) and four distinct RFLP banding patterns (280+400, 340+340, 280+470, and no digested amplicon of 750 bp) were observed. Genotype with PCR-RFLP patterns of 280+400 bp was predominated. The results indicated polymorphism in the investigated regions of coagulase gene. This polymorphism can be used for identification of S. aureus isolates and showing the epidemiological relationship among them. |
| first_indexed | 2024-04-11T15:25:03Z |
| format | Article |
| id | doaj.art-bb970f100be94b49ac0a66b271554699 |
| institution | Directory Open Access Journal |
| issn | 2008-9147 2717-3364 |
| language | English |
| last_indexed | 2024-04-11T15:25:03Z |
| publishDate | 2018-09-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Journal of Cell and Molecular Research |
| spelling | doaj.art-bb970f100be94b49ac0a66b2715546992022-12-22T04:16:16ZengFerdowsi University of MashhadJournal of Cell and Molecular Research2008-91472717-33642018-09-01101273110.22067/jcmr.v10i1.5273029408Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of IranMohammad Reza Izadpanah0Leila Asadpour1Rasht Branch, Islamic Azad UniversityRasht Branch, Islamic Azad UniversityStaphylococcus aureus is a common pathogen potentially able to cause a wide range of infectious diseases in human and animals and coagulase enzyme is one of the important virulence factors of this bacterium. Polymorphism of the coagulase encoding gene (coa) is one of the molecular-based typing methods of S. aureus isolates. In this study, the polymorphism of the coagulase gene among MRSA and MSSA isolates were investigated using PCR-RFLP analysis. To perform coagulase gene typing, the repeated units encoding hypervariable regions of coagulase gene of 30 clinical isolates of S. aureus were amplified by the PCR technique; this was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. In total two amplicons (680 bp and 750 bp) and four distinct RFLP banding patterns (280+400, 340+340, 280+470, and no digested amplicon of 750 bp) were observed. Genotype with PCR-RFLP patterns of 280+400 bp was predominated. The results indicated polymorphism in the investigated regions of coagulase gene. This polymorphism can be used for identification of S. aureus isolates and showing the epidemiological relationship among them.https://jcmr.um.ac.ir/article_29408_24d0460f48fd6f8a16f646661ef7db8b.pdfstaphylococcus aureuscoa genepcr-rflppolymorphism |
| spellingShingle | Mohammad Reza Izadpanah Leila Asadpour Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of Iran Journal of Cell and Molecular Research staphylococcus aureus coa gene pcr-rflp polymorphism |
| title | Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of Iran |
| title_full | Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of Iran |
| title_fullStr | Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of Iran |
| title_full_unstemmed | Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of Iran |
| title_short | Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of Iran |
| title_sort | investigation of coa gene polymorphism in clinical isolates of staphylococcus aureus in north of iran |
| topic | staphylococcus aureus coa gene pcr-rflp polymorphism |
| url | https://jcmr.um.ac.ir/article_29408_24d0460f48fd6f8a16f646661ef7db8b.pdf |
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