Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique
Antibacterial and osteogenic functionalization of titanium (Ti) implants will greatly expand their clinical indications in immediate implant therapy, accelerate osteointegration, and enhance long-term prognosis. We had recently shown that the high-energy shot peening (HESP)-assisted micro-arc oxidat...
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| Format: | Article |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.573464/full |
| _version_ | 1818966999097671680 |
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| author | Xinkun Shen Wenjia Hu Linchao Ping Chongxing Liu Lili Yao Zhennan Deng Gang Wu Gang Wu |
| author_facet | Xinkun Shen Wenjia Hu Linchao Ping Chongxing Liu Lili Yao Zhennan Deng Gang Wu Gang Wu |
| author_sort | Xinkun Shen |
| collection | DOAJ |
| description | Antibacterial and osteogenic functionalization of titanium (Ti) implants will greatly expand their clinical indications in immediate implant therapy, accelerate osteointegration, and enhance long-term prognosis. We had recently shown that the high-energy shot peening (HESP)-assisted micro-arc oxidation (MAO) significantly improved the bioactivity and coating stability of Ti-based substrates. In this study, we further functionalized Ti with antibacterial and osteogenic properties by doping silicon (Si) and/or copper (Cu) ions into HESP/MAO-treated coatings. Physicochemical characterization displayed that the doping of Si and Cu in HESP/MAO-treated coatings (Si/Cu-MAO) did not significantly change their surface topography, roughness, crystal structure, coating thickness, bonding strength, and wettability. The results of X-ray photoelectron spectroscopy (XPS) showed that Si and Cu in the Si/Cu-MAO coating was in the form of silicate radical (SiO32–) and bivalent copper (Cu2+), respectively. The total amounts of Si and Cu were about 13.5 and 5.8 μg/cm2, which released about 33.2 and 31.3% within 14 day, respectively. Compared with the control group (MAO), Si doping samples (MAO-Si) significantly increased the cell viability, alkaline phosphatase (ALP) activity, mineralization and osteogenic genes (ALP, collagen I and osteocalcin) expression of MC3T3-E1 cells. Furthermore, the addition of Cu presented good bactericidal property against both Staphylococcus aureus and Streptococcus mutans (even under the co-culture condition of bacteria and MC3T3-E1 cells): the bacteriostatic rate of both bacteria was over 95%. In conclusion, the novel bioactive Si/Cu-MAO coating with antibacterial and osteogenic properties is a promising functionalization method for orthopedic and dental implants, especially in the immediate implant treatment with an infected socket. |
| first_indexed | 2024-12-20T13:41:49Z |
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| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-20T13:41:49Z |
| publishDate | 2020-10-01 |
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| spelling | doaj.art-bb97718951574e0b8ab493a9e42e0e232022-12-21T19:38:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-10-01810.3389/fbioe.2020.573464573464Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation TechniqueXinkun Shen0Wenjia Hu1Linchao Ping2Chongxing Liu3Lili Yao4Zhennan Deng5Gang Wu6Gang Wu7School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam Movement Science, University of Amsterdam and Vrije University Amsterdam, Amsterdam, NetherlandDepartment of Oral and Maxillofacial Surgary/Pathology, Amsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universitetit Amsterdam, Amsterdam Movement Science, Amsterdam, NetherlandsAntibacterial and osteogenic functionalization of titanium (Ti) implants will greatly expand their clinical indications in immediate implant therapy, accelerate osteointegration, and enhance long-term prognosis. We had recently shown that the high-energy shot peening (HESP)-assisted micro-arc oxidation (MAO) significantly improved the bioactivity and coating stability of Ti-based substrates. In this study, we further functionalized Ti with antibacterial and osteogenic properties by doping silicon (Si) and/or copper (Cu) ions into HESP/MAO-treated coatings. Physicochemical characterization displayed that the doping of Si and Cu in HESP/MAO-treated coatings (Si/Cu-MAO) did not significantly change their surface topography, roughness, crystal structure, coating thickness, bonding strength, and wettability. The results of X-ray photoelectron spectroscopy (XPS) showed that Si and Cu in the Si/Cu-MAO coating was in the form of silicate radical (SiO32–) and bivalent copper (Cu2+), respectively. The total amounts of Si and Cu were about 13.5 and 5.8 μg/cm2, which released about 33.2 and 31.3% within 14 day, respectively. Compared with the control group (MAO), Si doping samples (MAO-Si) significantly increased the cell viability, alkaline phosphatase (ALP) activity, mineralization and osteogenic genes (ALP, collagen I and osteocalcin) expression of MC3T3-E1 cells. Furthermore, the addition of Cu presented good bactericidal property against both Staphylococcus aureus and Streptococcus mutans (even under the co-culture condition of bacteria and MC3T3-E1 cells): the bacteriostatic rate of both bacteria was over 95%. In conclusion, the novel bioactive Si/Cu-MAO coating with antibacterial and osteogenic properties is a promising functionalization method for orthopedic and dental implants, especially in the immediate implant treatment with an infected socket.https://www.frontiersin.org/article/10.3389/fbioe.2020.573464/fulltitaniumhigh-energy shot peeningmicro-arc oxidationantibacterialosteogenesis |
| spellingShingle | Xinkun Shen Wenjia Hu Linchao Ping Chongxing Liu Lili Yao Zhennan Deng Gang Wu Gang Wu Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique Frontiers in Bioengineering and Biotechnology titanium high-energy shot peening micro-arc oxidation antibacterial osteogenesis |
| title | Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique |
| title_full | Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique |
| title_fullStr | Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique |
| title_full_unstemmed | Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique |
| title_short | Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique |
| title_sort | antibacterial and osteogenic functionalization of titanium with silicon copper doped high energy shot peening assisted micro arc oxidation technique |
| topic | titanium high-energy shot peening micro-arc oxidation antibacterial osteogenesis |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.573464/full |
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