Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity
Infectious diseases constitute a problem of great importance for animal and human health, as well as the increasing bacterial resistance to antibiotics. In this context, medicinal plants emerge as an effective alternative to replace the use antibiotics. The essential oil (EO) of Minthostachys vertic...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021000013 |
| _version_ | 1819061872285974528 |
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| author | M.E. Cecchini C. Paoloni N. Campra N. Picco M.C. Grosso M.L. Soriano Perez F. Alustiza N. Cariddi R. Bellingeri |
| author_facet | M.E. Cecchini C. Paoloni N. Campra N. Picco M.C. Grosso M.L. Soriano Perez F. Alustiza N. Cariddi R. Bellingeri |
| author_sort | M.E. Cecchini |
| collection | DOAJ |
| description | Infectious diseases constitute a problem of great importance for animal and human health, as well as the increasing bacterial resistance to antibiotics. In this context, medicinal plants emerge as an effective alternative to replace the use antibiotics. The essential oil (EO) of Minthostachys verticillata (Griseb.) Epling (Lamiaceae) has demonstrated a strong antimicrobial activity. However, its instability and hydrophobicity under normal storage conditions are limitations to its use. Nanoemulsion technology is an excellent way to solubilize, microencapsulate, and protect this compound. This study aimed to obtain a nanoemulsion based on M. verticillata EO and evaluate its antibacterial activity against Staphylococcus aureus. The EO was obtained by steam distillation. Identification and quantification of their components were determined by GC-MS revealing that the dominated chemical group was oxygenated monoterpenes. Nanoemulsions (NE) were characterized by measuring pH, transmittance, separation percentage, release profile, and morphology. The effect of NE on the growth of S. aureus and cyto-compatibility was also evaluated. The results showed that NE containing a higher percentage of tween 20 exhibited higher stability with an approximated droplet size of 10 nm. The effect of encapsulation process was evaluated by GC-MS revealing that the volatile components in EO were no affected. After 24 h, 74.24 ± 0.75% of EO was released from NE and the antibacterial activity of EO was enhanced considerably by its encapsulation. The incubation of S. aureus with the NE and pure EO, show a bacterial growth inhibition of 58.87% ± 0.99 and 46.72% ± 3.32 (p < 0.05), respectively. In addition, nanoemulsión did not cause toxicity to porcine and equine red blood cells. The results obtained showed that NE could be a potential vehicle for M. verticillata EO with promissory properties to emerge as a tool for developing advanced therapies to control and combat infections. |
| first_indexed | 2024-12-21T14:49:47Z |
| format | Article |
| id | doaj.art-bb9b0805888543aba3a67995918f3221 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-21T14:49:47Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-bb9b0805888543aba3a67995918f32212022-12-21T18:59:55ZengElsevierHeliyon2405-84402021-01-0171e05896Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activityM.E. Cecchini0C. Paoloni1N. Campra2N. Picco3M.C. Grosso4M.L. Soriano Perez5F. Alustiza6N. Cariddi7R. Bellingeri8Universidad Nacional de Río Cuarto, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Departamento de Microbiología e Inmunología, Laboratorio de Inmunología, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, Argentina; Instituto de Biotecnología Ambiental y Salud (INBIAS) CONICET, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, ArgentinaUniversidad Nacional de Río Cuarto, Facultad de Agronomía y Veterinaria, Departamento de Anatomía Animal, Laboratorio de Biotecnología Animal, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, ArgentinaUniversidad Nacional de Río Cuarto, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Departamento de Microbiología e Inmunología, Laboratorio de Inmunología, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, Argentina; Instituto de Biotecnología Ambiental y Salud (INBIAS) CONICET, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, ArgentinaUniversidad Nacional de Río Cuarto, Facultad de Agronomía y Veterinaria, Departamento de Anatomía Animal, Laboratorio de Biotecnología Animal, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, ArgentinaUniversidad Nacional de Río Cuarto, Facultad de Agronomía y Veterinaria, Departamento de Anatomía Animal, Laboratorio de Biotecnología Animal, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, ArgentinaInstituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Marcos Juárez, Marcos Juárez, X2580, Córdoba, ArgentinaInstituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Marcos Juárez, Marcos Juárez, X2580, Córdoba, ArgentinaUniversidad Nacional de Río Cuarto, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Departamento de Microbiología e Inmunología, Laboratorio de Inmunología, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, Argentina; Instituto de Biotecnología Ambiental y Salud (INBIAS) CONICET, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, ArgentinaUniversidad Nacional de Río Cuarto, Facultad de Agronomía y Veterinaria, Departamento de Anatomía Animal, Laboratorio de Biotecnología Animal, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, Argentina; Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados (IITEMA) CONICET, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba, 5800, Argentina; Corresponding author.Infectious diseases constitute a problem of great importance for animal and human health, as well as the increasing bacterial resistance to antibiotics. In this context, medicinal plants emerge as an effective alternative to replace the use antibiotics. The essential oil (EO) of Minthostachys verticillata (Griseb.) Epling (Lamiaceae) has demonstrated a strong antimicrobial activity. However, its instability and hydrophobicity under normal storage conditions are limitations to its use. Nanoemulsion technology is an excellent way to solubilize, microencapsulate, and protect this compound. This study aimed to obtain a nanoemulsion based on M. verticillata EO and evaluate its antibacterial activity against Staphylococcus aureus. The EO was obtained by steam distillation. Identification and quantification of their components were determined by GC-MS revealing that the dominated chemical group was oxygenated monoterpenes. Nanoemulsions (NE) were characterized by measuring pH, transmittance, separation percentage, release profile, and morphology. The effect of NE on the growth of S. aureus and cyto-compatibility was also evaluated. The results showed that NE containing a higher percentage of tween 20 exhibited higher stability with an approximated droplet size of 10 nm. The effect of encapsulation process was evaluated by GC-MS revealing that the volatile components in EO were no affected. After 24 h, 74.24 ± 0.75% of EO was released from NE and the antibacterial activity of EO was enhanced considerably by its encapsulation. The incubation of S. aureus with the NE and pure EO, show a bacterial growth inhibition of 58.87% ± 0.99 and 46.72% ± 3.32 (p < 0.05), respectively. In addition, nanoemulsión did not cause toxicity to porcine and equine red blood cells. The results obtained showed that NE could be a potential vehicle for M. verticillata EO with promissory properties to emerge as a tool for developing advanced therapies to control and combat infections.http://www.sciencedirect.com/science/article/pii/S2405844021000013Minthostachys verticillataEssential oilNanoemulsionAntibacterial activity |
| spellingShingle | M.E. Cecchini C. Paoloni N. Campra N. Picco M.C. Grosso M.L. Soriano Perez F. Alustiza N. Cariddi R. Bellingeri Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity Heliyon Minthostachys verticillata Essential oil Nanoemulsion Antibacterial activity |
| title | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_full | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_fullStr | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_full_unstemmed | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_short | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_sort | nanoemulsion of minthostachys verticillata essential oil in vitro evaluation of its antibacterial activity |
| topic | Minthostachys verticillata Essential oil Nanoemulsion Antibacterial activity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021000013 |
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