Development and Validation of an In-House Real-Time Reverse-Transcriptase Polymerase Chain Reaction Assay for SARS-CoV-2 Omicron Lineage Subtyping between BA.1 and BA.2

Development and Validation of an In-House Real-Time Reverse-Transcriptase Polymerase Chain Reaction Assay for SARS-CoV-2 Omicron Lineage Subtyping between BA.1 and BA.2

In order to rapidly differentiate sublineages BA.1 and BA.2 of the SARS-CoV-2 variant of concern Omicron, we developed a real-time reverse-transcriptase polymerase chain reaction to target the discriminatory spike protein deletion at amino acid position 69–70 (S:del69–70). Compared to the gold stand...

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Bibliographic Details
Main Authors: David Pham, Susan Maddocks, Dominic E. Dwyer, Vitali Sintchenko, Jen Kok, Rebecca J. Rockett
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Viruses
Subjects:
SARS-CoV-2
variants of concern
COVID-19
real-time RT-PCR
Omicron
sublineage
Online Access:https://www.mdpi.com/1999-4915/14/8/1760

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