Detection of herbivory: eDNA detection from feeding marks on leaves
Abstract Many techniques have been developed to investigate the interactions between plants and herbivorous insects in natural environments and are generally used to determine either (a) which plant species are eaten by a specific herbivorous insect or (b) which herbivorous insect species are herbiv...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-10-01
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| Series: | Environmental DNA |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/edn3.113 |
| _version_ | 1828832963216801792 |
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| author | Aoi Kudoh Toshifumi Minamoto Satoshi Yamamoto |
| author_facet | Aoi Kudoh Toshifumi Minamoto Satoshi Yamamoto |
| author_sort | Aoi Kudoh |
| collection | DOAJ |
| description | Abstract Many techniques have been developed to investigate the interactions between plants and herbivorous insects in natural environments and are generally used to determine either (a) which plant species are eaten by a specific herbivorous insect or (b) which herbivorous insect species are herbivores of a specific plant. The former problem is usually addressed by the direct observation of feeding and microscopic observation of gut contents and excrements, as well as the application of DNA‐barcoding techniques. However, the latter problem has typically been addressed using time‐consuming methods, such as direct observation and rearing. Therefore, more efficient techniques are needed for identifying and quantifying the interactions of plants with herbivorous insects. The present study demonstrates that the environmental DNA (eDNA) of herbivorous insects can be recovered from leaves with external foliage feeding marks. Mitochondrial DNA fragments of herbivorous insects were detected from insect‐exposed leaves using primer sets that amplified the DNA of target species. The amplification rate of the herbivorous insect DNA was positively associated with the rim length of feeding marks, which suggests that most of the insect DNA came from the feeding marks. Additionally, we showed that this method has the potential to detect eDNA from field‐collected leaves. This time‐efficient approach will contribute to the detection of plant–insect herbivore interactions. |
| first_indexed | 2024-12-12T17:05:02Z |
| format | Article |
| id | doaj.art-bb9fedebff1d416f819ec76f245dfce3 |
| institution | Directory Open Access Journal |
| issn | 2637-4943 |
| language | English |
| last_indexed | 2024-12-12T17:05:02Z |
| publishDate | 2020-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Environmental DNA |
| spelling | doaj.art-bb9fedebff1d416f819ec76f245dfce32022-12-22T00:18:01ZengWileyEnvironmental DNA2637-49432020-10-012462763410.1002/edn3.113Detection of herbivory: eDNA detection from feeding marks on leavesAoi Kudoh0Toshifumi Minamoto1Satoshi Yamamoto2Faculty of Agriculture Kyoto University Kyoto JapanGraduate School of Human Development and Environment Kobe University Kobe JapanDepartment of Zoology Graduate School of Science Kyoto University Kyoto JapanAbstract Many techniques have been developed to investigate the interactions between plants and herbivorous insects in natural environments and are generally used to determine either (a) which plant species are eaten by a specific herbivorous insect or (b) which herbivorous insect species are herbivores of a specific plant. The former problem is usually addressed by the direct observation of feeding and microscopic observation of gut contents and excrements, as well as the application of DNA‐barcoding techniques. However, the latter problem has typically been addressed using time‐consuming methods, such as direct observation and rearing. Therefore, more efficient techniques are needed for identifying and quantifying the interactions of plants with herbivorous insects. The present study demonstrates that the environmental DNA (eDNA) of herbivorous insects can be recovered from leaves with external foliage feeding marks. Mitochondrial DNA fragments of herbivorous insects were detected from insect‐exposed leaves using primer sets that amplified the DNA of target species. The amplification rate of the herbivorous insect DNA was positively associated with the rim length of feeding marks, which suggests that most of the insect DNA came from the feeding marks. Additionally, we showed that this method has the potential to detect eDNA from field‐collected leaves. This time‐efficient approach will contribute to the detection of plant–insect herbivore interactions.https://doi.org/10.1002/edn3.113herbivoryinsectlarvaleafreal‐time PCRsaliva |
| spellingShingle | Aoi Kudoh Toshifumi Minamoto Satoshi Yamamoto Detection of herbivory: eDNA detection from feeding marks on leaves Environmental DNA herbivory insect larva leaf real‐time PCR saliva |
| title | Detection of herbivory: eDNA detection from feeding marks on leaves |
| title_full | Detection of herbivory: eDNA detection from feeding marks on leaves |
| title_fullStr | Detection of herbivory: eDNA detection from feeding marks on leaves |
| title_full_unstemmed | Detection of herbivory: eDNA detection from feeding marks on leaves |
| title_short | Detection of herbivory: eDNA detection from feeding marks on leaves |
| title_sort | detection of herbivory edna detection from feeding marks on leaves |
| topic | herbivory insect larva leaf real‐time PCR saliva |
| url | https://doi.org/10.1002/edn3.113 |
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