Rapid Screening of toxigenic and non-toxigenic V. cholera from clinical and environmental samples in Iraq by PCR
Zonnla occludens toxin (zot) is one of the major virulence factors of Vibrio cholera. The detection of zot producing V. cholerae using conventional culture, biochemical and immunological based assays is time-consuming laborious, and requiring more than three days to perform. In this work aspecific...
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| Format: | Article |
| Language: | English |
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College of Pharmacy / Mustansiriyah University
2013-06-01
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| Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
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| Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/184 |
| _version_ | 1797223948106072064 |
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| author | Tahreer Hadi Saleh Alnaddawi Sadik Abdulridha Gatea Alkaabi Waffa Hassan Muslem |
| author_facet | Tahreer Hadi Saleh Alnaddawi Sadik Abdulridha Gatea Alkaabi Waffa Hassan Muslem |
| author_sort | Tahreer Hadi Saleh Alnaddawi |
| collection | DOAJ |
| description |
Zonnla occludens toxin (zot) is one of the major virulence factors of Vibrio cholera. The detection of zot producing V. cholerae using conventional culture, biochemical and immunological based assays is time-consuming laborious, and requiring more than three days to perform. In this work aspecific primers for zot gene were used for detection of V. cholerae that isolated from clinical and environmental samples.
The results revealed that 12 of 15 clinical isolates were Ol serogroup, 6 isolates belonged to each of serotypes ogawa and lnaba and 3 isolates belonged to the serotype Non/ Ol, and all environmental isolates (5 isolates) were belonged to the serotype Non/ O1. Few colonies of V.
cholerae were suspended in nutrient broth and used as few plates in PCR reaction for the detection of zot gene, The 947 bp sequence of a gene that codes for the zonula occludens toxin was amplified by PCR. Direct use of V. cholerae pure culture for PCR replaces the need for DNA extraction or boiling.
PCR results showed that all clinical (Ol) isolates of V. cholerae contain gene namely zot, while the Non/ O1 isolates that had been isolated from clinical sources, as well as, that isolated from environment showed no existence for zot gene.
The results of Molecular epidemiology study indicated that the source of outbreaks of 2007, 2008 and 2009 is the same all over Iraq, according to the PCR profile.
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| first_indexed | 2024-04-24T13:45:20Z |
| format | Article |
| id | doaj.art-bba14b4c15ac4739bf7acdeac6c094fd |
| institution | Directory Open Access Journal |
| issn | 1815-0993 2959-183X |
| language | English |
| last_indexed | 2024-04-24T13:45:20Z |
| publishDate | 2013-06-01 |
| publisher | College of Pharmacy / Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| spelling | doaj.art-bba14b4c15ac4739bf7acdeac6c094fd2024-04-04T06:58:40ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2013-06-0113110.32947/ajps.v13i1.184Rapid Screening of toxigenic and non-toxigenic V. cholera from clinical and environmental samples in Iraq by PCRTahreer Hadi Saleh Alnaddawi0Sadik Abdulridha Gatea Alkaabi1Waffa Hassan Muslem2Biology Department, Collage science, AlMustansiriyah University*Biology Department, Collage science, University of BaghdadBiology Department, Collage science, AlMustansiriyah University Zonnla occludens toxin (zot) is one of the major virulence factors of Vibrio cholera. The detection of zot producing V. cholerae using conventional culture, biochemical and immunological based assays is time-consuming laborious, and requiring more than three days to perform. In this work aspecific primers for zot gene were used for detection of V. cholerae that isolated from clinical and environmental samples. The results revealed that 12 of 15 clinical isolates were Ol serogroup, 6 isolates belonged to each of serotypes ogawa and lnaba and 3 isolates belonged to the serotype Non/ Ol, and all environmental isolates (5 isolates) were belonged to the serotype Non/ O1. Few colonies of V. cholerae were suspended in nutrient broth and used as few plates in PCR reaction for the detection of zot gene, The 947 bp sequence of a gene that codes for the zonula occludens toxin was amplified by PCR. Direct use of V. cholerae pure culture for PCR replaces the need for DNA extraction or boiling. PCR results showed that all clinical (Ol) isolates of V. cholerae contain gene namely zot, while the Non/ O1 isolates that had been isolated from clinical sources, as well as, that isolated from environment showed no existence for zot gene. The results of Molecular epidemiology study indicated that the source of outbreaks of 2007, 2008 and 2009 is the same all over Iraq, according to the PCR profile. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/184toxigenic, non-toxigenic, pcr, V. cholera |
| spellingShingle | Tahreer Hadi Saleh Alnaddawi Sadik Abdulridha Gatea Alkaabi Waffa Hassan Muslem Rapid Screening of toxigenic and non-toxigenic V. cholera from clinical and environmental samples in Iraq by PCR Al-Mustansiriyah Journal of Pharmaceutical Sciences toxigenic, non-toxigenic, pcr, V. cholera |
| title | Rapid Screening of toxigenic and non-toxigenic V. cholera from clinical and environmental samples in Iraq by PCR |
| title_full | Rapid Screening of toxigenic and non-toxigenic V. cholera from clinical and environmental samples in Iraq by PCR |
| title_fullStr | Rapid Screening of toxigenic and non-toxigenic V. cholera from clinical and environmental samples in Iraq by PCR |
| title_full_unstemmed | Rapid Screening of toxigenic and non-toxigenic V. cholera from clinical and environmental samples in Iraq by PCR |
| title_short | Rapid Screening of toxigenic and non-toxigenic V. cholera from clinical and environmental samples in Iraq by PCR |
| title_sort | rapid screening of toxigenic and non toxigenic v cholera from clinical and environmental samples in iraq by pcr |
| topic | toxigenic, non-toxigenic, pcr, V. cholera |
| url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/184 |
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