Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in Vietnam
Sponge-associated prokaryotic diversity has been studied from a wide range of marine environments across the globe. However, for certain regions, e.g., Vietnam, Thailand, Cambodia, and Singapore, an overview of the sponge-associated prokaryotic communities is still pending. In this study we characte...
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PeerJ Inc.
2018-06-01
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| author | Ton That Huu Dat Georg Steinert Nguyen Thi Kim Cuc Hauke Smidt Detmer Sipkema |
| author_facet | Ton That Huu Dat Georg Steinert Nguyen Thi Kim Cuc Hauke Smidt Detmer Sipkema |
| author_sort | Ton That Huu Dat |
| collection | DOAJ |
| description | Sponge-associated prokaryotic diversity has been studied from a wide range of marine environments across the globe. However, for certain regions, e.g., Vietnam, Thailand, Cambodia, and Singapore, an overview of the sponge-associated prokaryotic communities is still pending. In this study we characterized the prokaryotic communities from 27 specimens, comprising 18 marine sponge species, sampled from the central coastal region of Vietnam. Illumina MiSeq sequencing of 16S ribosomal RNA (rRNA) gene fragments was used to investigate sponge-associated bacterial and archaeal diversity. Overall, 14 bacterial phyla and one archaeal phylum were identified among all 27 samples. The phylum Proteobacteria was present in all sponges and the most prevalent phylum in 15 out of 18 sponge species, albeit with pronounced differences at the class level. In contrast, Chloroflexi was the most abundant phylum in Halichondria sp., whereas Spirastrella sp. and Dactylospongia sp. were dominated by Actinobacteria. Several bacterial phyla such as Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Deferribacteres, Gemmatimonadetes, and Nitrospirae were found in two-thirds of the sponge species. Moreover, the phylum Thaumarchaeota (Archaea), which is known to comprise nitrifying archaea, was highly abundant among the majority of the 18 investigated sponge species. Altogether, this study demonstrates that the diversity of prokaryotic communities associated with Vietnamese sponges is comparable to sponge-prokaryotic assemblages from well-documented regions. Furthermore, the phylogenetically divergent sponges hosted species-specific prokaryotic communities, thus demonstrating the influence of host identity on the composition and diversity of the associated communities. Therefore, this high-throughput 16S rRNA gene amplicon analysis of Vietnamese sponge-prokaryotic communities provides a foundation for future studies on sponge symbiont function and sponge-derived bioactive compounds from this region. |
| first_indexed | 2024-03-09T06:36:30Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T06:36:30Z |
| publishDate | 2018-06-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-bba52f67ea7a4308b8c77b6d656bd7082023-12-03T10:58:16ZengPeerJ Inc.PeerJ2167-83592018-06-016e497010.7717/peerj.4970Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in VietnamTon That Huu Dat0Georg Steinert1Nguyen Thi Kim Cuc2Hauke Smidt3Detmer Sipkema4Mientrung Institute for Scientific Research, Vietnam Academy of Science and Technology, Hanoi, VietnamLaboratory of Microbiology, Wageningen University & Research, Wageningen, The NetherlandsInstitute of Marine Biochemistry, Vietnam Academy of Science and Technology, Ha Noi, VietnamLaboratory of Microbiology, Wageningen University & Research, Wageningen, The NetherlandsLaboratory of Microbiology, Wageningen University & Research, Wageningen, The NetherlandsSponge-associated prokaryotic diversity has been studied from a wide range of marine environments across the globe. However, for certain regions, e.g., Vietnam, Thailand, Cambodia, and Singapore, an overview of the sponge-associated prokaryotic communities is still pending. In this study we characterized the prokaryotic communities from 27 specimens, comprising 18 marine sponge species, sampled from the central coastal region of Vietnam. Illumina MiSeq sequencing of 16S ribosomal RNA (rRNA) gene fragments was used to investigate sponge-associated bacterial and archaeal diversity. Overall, 14 bacterial phyla and one archaeal phylum were identified among all 27 samples. The phylum Proteobacteria was present in all sponges and the most prevalent phylum in 15 out of 18 sponge species, albeit with pronounced differences at the class level. In contrast, Chloroflexi was the most abundant phylum in Halichondria sp., whereas Spirastrella sp. and Dactylospongia sp. were dominated by Actinobacteria. Several bacterial phyla such as Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Deferribacteres, Gemmatimonadetes, and Nitrospirae were found in two-thirds of the sponge species. Moreover, the phylum Thaumarchaeota (Archaea), which is known to comprise nitrifying archaea, was highly abundant among the majority of the 18 investigated sponge species. Altogether, this study demonstrates that the diversity of prokaryotic communities associated with Vietnamese sponges is comparable to sponge-prokaryotic assemblages from well-documented regions. Furthermore, the phylogenetically divergent sponges hosted species-specific prokaryotic communities, thus demonstrating the influence of host identity on the composition and diversity of the associated communities. Therefore, this high-throughput 16S rRNA gene amplicon analysis of Vietnamese sponge-prokaryotic communities provides a foundation for future studies on sponge symbiont function and sponge-derived bioactive compounds from this region.https://peerj.com/articles/4970.pdf16S rRNAProkaryotic diversityVietnamSymbiosisPorifera |
| spellingShingle | Ton That Huu Dat Georg Steinert Nguyen Thi Kim Cuc Hauke Smidt Detmer Sipkema Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in Vietnam PeerJ 16S rRNA Prokaryotic diversity Vietnam Symbiosis Porifera |
| title | Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in Vietnam |
| title_full | Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in Vietnam |
| title_fullStr | Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in Vietnam |
| title_full_unstemmed | Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in Vietnam |
| title_short | Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in Vietnam |
| title_sort | archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in vietnam |
| topic | 16S rRNA Prokaryotic diversity Vietnam Symbiosis Porifera |
| url | https://peerj.com/articles/4970.pdf |
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