Identification of Key Pathways and Genes in L4 Dorsal Root Ganglion (DRG) After Sciatic Nerve Injury via Microarray Analysis
Background: Peripheral nerve injury (PNI) has devastating consequences. Dorsal root ganglion as a pivotal locus participates in the process of neuropathic pain and nerve regeneration. In recent years, gene sequencing technology has seen rapid rise in the biomedicine field. So, we attempt to gain ins...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-02-01
|
| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08941939.2018.1452996 |
| _version_ | 1797684694387523584 |
|---|---|
| author | He Zhao Li-Jun Duan Qing-Ling Sun Yu-Shan Gao Yong-Dong Yang Xiang-Sheng Tang Ding-Yan Zhao Yang Xiong Zhen-Guo Hu Chuan-Hong Li Si-Xue Chen Tao Liu Xing Yu |
| author_facet | He Zhao Li-Jun Duan Qing-Ling Sun Yu-Shan Gao Yong-Dong Yang Xiang-Sheng Tang Ding-Yan Zhao Yang Xiong Zhen-Guo Hu Chuan-Hong Li Si-Xue Chen Tao Liu Xing Yu |
| author_sort | He Zhao |
| collection | DOAJ |
| description | Background: Peripheral nerve injury (PNI) has devastating consequences. Dorsal root ganglion as a pivotal locus participates in the process of neuropathic pain and nerve regeneration. In recent years, gene sequencing technology has seen rapid rise in the biomedicine field. So, we attempt to gain insight into in the mechanism of neuropathic pain and nerve regeneration in the transcriptional level and to explore novel genes through bioinformatics analysis. Methods: The gene expression profiles of GSE96051 were downloaded from GEO database. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses were performed, and protein–protein interaction (PPI) network of the differentially expressed genes (DEGs) was constructed by Cytoscape software. Results: Our results showed that both IL-6 and Jun genes and the signaling pathway of MAPK, apoptosis, P53 present their vital modulatory role in nerve regeneration and neuropathic pain. Noteworthy, 13 hub genes associated with neuropathic pain and nerve regeneration, including Ccl12, Ppp1r15a, Cdkn1a, Atf3, Nts, Dusp1, Ccl7, Csf, Gadd45a, Serpine1, Timp1 were rarely reported in PubMed database, these genes may provide us the new orientation in experimental research and clinical study. Conclusions: Our results may provide more deep insight into the mechanism and a promising therapeutic target. The next step is to put our emphasis on an experiment level and to verify the novel genes from 13 hub genes. |
| first_indexed | 2024-03-12T00:34:26Z |
| format | Article |
| id | doaj.art-bba6a95da29c4065885af849a4293f72 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:34:26Z |
| publishDate | 2020-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-bba6a95da29c4065885af849a4293f722023-09-15T10:07:28ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532020-02-0133217218010.1080/08941939.2018.14529961452996Identification of Key Pathways and Genes in L4 Dorsal Root Ganglion (DRG) After Sciatic Nerve Injury via Microarray AnalysisHe Zhao0Li-Jun Duan1Qing-Ling Sun2Yu-Shan Gao3Yong-Dong Yang4Xiang-Sheng Tang5Ding-Yan Zhao6Yang Xiong7Zhen-Guo Hu8Chuan-Hong Li9Si-Xue Chen10Tao Liu11Xing Yu12Beijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBackground: Peripheral nerve injury (PNI) has devastating consequences. Dorsal root ganglion as a pivotal locus participates in the process of neuropathic pain and nerve regeneration. In recent years, gene sequencing technology has seen rapid rise in the biomedicine field. So, we attempt to gain insight into in the mechanism of neuropathic pain and nerve regeneration in the transcriptional level and to explore novel genes through bioinformatics analysis. Methods: The gene expression profiles of GSE96051 were downloaded from GEO database. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses were performed, and protein–protein interaction (PPI) network of the differentially expressed genes (DEGs) was constructed by Cytoscape software. Results: Our results showed that both IL-6 and Jun genes and the signaling pathway of MAPK, apoptosis, P53 present their vital modulatory role in nerve regeneration and neuropathic pain. Noteworthy, 13 hub genes associated with neuropathic pain and nerve regeneration, including Ccl12, Ppp1r15a, Cdkn1a, Atf3, Nts, Dusp1, Ccl7, Csf, Gadd45a, Serpine1, Timp1 were rarely reported in PubMed database, these genes may provide us the new orientation in experimental research and clinical study. Conclusions: Our results may provide more deep insight into the mechanism and a promising therapeutic target. The next step is to put our emphasis on an experiment level and to verify the novel genes from 13 hub genes.http://dx.doi.org/10.1080/08941939.2018.1452996dorsal root ganglionsciatic nerve injurymicroarray analysis |
| spellingShingle | He Zhao Li-Jun Duan Qing-Ling Sun Yu-Shan Gao Yong-Dong Yang Xiang-Sheng Tang Ding-Yan Zhao Yang Xiong Zhen-Guo Hu Chuan-Hong Li Si-Xue Chen Tao Liu Xing Yu Identification of Key Pathways and Genes in L4 Dorsal Root Ganglion (DRG) After Sciatic Nerve Injury via Microarray Analysis Journal of Investigative Surgery dorsal root ganglion sciatic nerve injury microarray analysis |
| title | Identification of Key Pathways and Genes in L4 Dorsal Root Ganglion (DRG) After Sciatic Nerve Injury via Microarray Analysis |
| title_full | Identification of Key Pathways and Genes in L4 Dorsal Root Ganglion (DRG) After Sciatic Nerve Injury via Microarray Analysis |
| title_fullStr | Identification of Key Pathways and Genes in L4 Dorsal Root Ganglion (DRG) After Sciatic Nerve Injury via Microarray Analysis |
| title_full_unstemmed | Identification of Key Pathways and Genes in L4 Dorsal Root Ganglion (DRG) After Sciatic Nerve Injury via Microarray Analysis |
| title_short | Identification of Key Pathways and Genes in L4 Dorsal Root Ganglion (DRG) After Sciatic Nerve Injury via Microarray Analysis |
| title_sort | identification of key pathways and genes in l4 dorsal root ganglion drg after sciatic nerve injury via microarray analysis |
| topic | dorsal root ganglion sciatic nerve injury microarray analysis |
| url | http://dx.doi.org/10.1080/08941939.2018.1452996 |
| work_keys_str_mv | AT hezhao identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT lijunduan identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT qinglingsun identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT yushangao identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT yongdongyang identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT xiangshengtang identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT dingyanzhao identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT yangxiong identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT zhenguohu identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT chuanhongli identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT sixuechen identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT taoliu identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis AT xingyu identificationofkeypathwaysandgenesinl4dorsalrootgangliondrgaftersciaticnerveinjuryviamicroarrayanalysis |