Endothelium in spots--high-content imaging of lipid rafts clusters in db/db mice.
Lipid rafts (LRs) are dynamic, sterol- and sphingolipid-enriched nanodomains involved in the regulation of cellular functions and signal transduction, that upon stimuli, via (e.g. association of raft proteins and lipids), may cluster into domains of submicron or micron scale. Up to date, however, li...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4148353?pdf=render |
| _version_ | 1818561305224675328 |
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| author | Marta Pilarczyk Lukasz Mateuszuk Anna Rygula Mariusz Kepczynski Stefan Chlopicki Malgorzata Baranska Agnieszka Kaczor |
| author_facet | Marta Pilarczyk Lukasz Mateuszuk Anna Rygula Mariusz Kepczynski Stefan Chlopicki Malgorzata Baranska Agnieszka Kaczor |
| author_sort | Marta Pilarczyk |
| collection | DOAJ |
| description | Lipid rafts (LRs) are dynamic, sterol- and sphingolipid-enriched nanodomains involved in the regulation of cellular functions and signal transduction, that upon stimuli, via (e.g. association of raft proteins and lipids), may cluster into domains of submicron or micron scale. Up to date, however, lipid raft clusters were observed only under artificially promoted conditions and their formation in vivo has not been confirmed. Using non-destructive approach involving Raman and Atomic Force Microscopy imaging we demonstrated the presence of clustered lipid rafts in endothelium of the aorta of the db/db mice that represent a reliable murine model of type 2 diabetes. The raft clusters in the aorta of diabetic mice were shown to occupy a considerably larger (about 10-fold) area of endothelial cells surface as compared to the control. Observation of pathology-promoted LRs confirms that the cellular increase of lipid content results in clustering of LRs. Clustering of LRs leads to the formation of assemblies with diameters up to 3 micrometers and increased lipid character. This massive clustering of lipid rafts in diabetes may trigger a signaling cascade leading to vascular inflammation. |
| first_indexed | 2024-12-14T00:49:07Z |
| format | Article |
| id | doaj.art-bba95e5ae9f44a3484b1a91dfbb3a137 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T00:49:07Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bba95e5ae9f44a3484b1a91dfbb3a1372022-12-21T23:23:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10606510.1371/journal.pone.0106065Endothelium in spots--high-content imaging of lipid rafts clusters in db/db mice.Marta PilarczykLukasz MateuszukAnna RygulaMariusz KepczynskiStefan ChlopickiMalgorzata BaranskaAgnieszka KaczorLipid rafts (LRs) are dynamic, sterol- and sphingolipid-enriched nanodomains involved in the regulation of cellular functions and signal transduction, that upon stimuli, via (e.g. association of raft proteins and lipids), may cluster into domains of submicron or micron scale. Up to date, however, lipid raft clusters were observed only under artificially promoted conditions and their formation in vivo has not been confirmed. Using non-destructive approach involving Raman and Atomic Force Microscopy imaging we demonstrated the presence of clustered lipid rafts in endothelium of the aorta of the db/db mice that represent a reliable murine model of type 2 diabetes. The raft clusters in the aorta of diabetic mice were shown to occupy a considerably larger (about 10-fold) area of endothelial cells surface as compared to the control. Observation of pathology-promoted LRs confirms that the cellular increase of lipid content results in clustering of LRs. Clustering of LRs leads to the formation of assemblies with diameters up to 3 micrometers and increased lipid character. This massive clustering of lipid rafts in diabetes may trigger a signaling cascade leading to vascular inflammation.http://europepmc.org/articles/PMC4148353?pdf=render |
| spellingShingle | Marta Pilarczyk Lukasz Mateuszuk Anna Rygula Mariusz Kepczynski Stefan Chlopicki Malgorzata Baranska Agnieszka Kaczor Endothelium in spots--high-content imaging of lipid rafts clusters in db/db mice. PLoS ONE |
| title | Endothelium in spots--high-content imaging of lipid rafts clusters in db/db mice. |
| title_full | Endothelium in spots--high-content imaging of lipid rafts clusters in db/db mice. |
| title_fullStr | Endothelium in spots--high-content imaging of lipid rafts clusters in db/db mice. |
| title_full_unstemmed | Endothelium in spots--high-content imaging of lipid rafts clusters in db/db mice. |
| title_short | Endothelium in spots--high-content imaging of lipid rafts clusters in db/db mice. |
| title_sort | endothelium in spots high content imaging of lipid rafts clusters in db db mice |
| url | http://europepmc.org/articles/PMC4148353?pdf=render |
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