Molecular cystoscopy: Micro-RNAs could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladder
Introduction: Normal-looking mucosa may harbor genetic changes preceding a visible tumor. This study was aimed at exploring the role of the quantitative expression of micro-RNAs (miRNAs) in bladder cancer tissue in comparison with normal mucosa and healthy controls (HCs) as a molecular marker. Mate...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Indian Journal of Urology |
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| Online Access: | http://www.indianjurol.com/article.asp?issn=0970-1591;year=2016;volume=32;issue=2;spage=149;epage=153;aulast=Mitash |
| _version_ | 1818845543489601536 |
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| author | Nilay Mitash Shalini Agnihotri Balraj Mittal Swasti Tiwari Anil Mandhani |
| author_facet | Nilay Mitash Shalini Agnihotri Balraj Mittal Swasti Tiwari Anil Mandhani |
| author_sort | Nilay Mitash |
| collection | DOAJ |
| description | Introduction: Normal-looking mucosa may harbor genetic changes preceding a visible tumor. This study was aimed at exploring the role of the quantitative expression of micro-RNAs (miRNAs) in bladder cancer tissue in comparison with normal mucosa and healthy controls (HCs) as a molecular marker.
Materials and Methods: Between October 2011 to December 2012, tissue from the bladder tumor of 21 patients (cases tumor, CT), normal mucosa (case control, CC) of the same patients (n-21) and normal bladder mucosa from 10 HCs were obtained. miRNAs of angiogenesis, endothelial mesenchymal transition and apoptosis were quantified using stem-loop RT Taq Man polymerase chain reaction. Statistical analysis was performed using the Chi square and independent sample T tests by using SPSS version 16.
Results: The mean age of the patients and controls were 55.41 ± 11.03 and 52.14 ± 13.04 years. miR-21, miR-205, miR-126, miR-10b and miR-200a were highly expressed in CT (P < 0.027, <0.048, <0.025, <0.029 and < 0.005) as compared with HC. Expression of miR-21 and miR-129 were both correlated with grade and stage (P = 0.001 and < 0.009, respectively) and the level of expression was different in the same grade of non-muscle invasive tumors. The fold change of miR129, miR205 and miR200a was significantly higher in the normal-looking mucosa of bladder tumor patients than the HC (P < 0.005).
Conclusion: Expression of miR129, miR205 and miR200a in the normal-looking mucosa of bladder cancer patients was significantly higher than the normal mucosa of a HC. This may help in predicting recurrence and formulating the follow-up strategy. |
| first_indexed | 2024-12-19T05:31:20Z |
| format | Article |
| id | doaj.art-bbaa70db392f418eb0ac008f20ac9360 |
| institution | Directory Open Access Journal |
| issn | 0970-1591 1998-3824 |
| language | English |
| last_indexed | 2024-12-19T05:31:20Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Urology |
| spelling | doaj.art-bbaa70db392f418eb0ac008f20ac93602022-12-21T20:34:14ZengWolters Kluwer Medknow PublicationsIndian Journal of Urology0970-15911998-38242016-01-0132214915310.4103/0970-1591.174775Molecular cystoscopy: Micro-RNAs could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladderNilay MitashShalini AgnihotriBalraj MittalSwasti TiwariAnil MandhaniIntroduction: Normal-looking mucosa may harbor genetic changes preceding a visible tumor. This study was aimed at exploring the role of the quantitative expression of micro-RNAs (miRNAs) in bladder cancer tissue in comparison with normal mucosa and healthy controls (HCs) as a molecular marker. Materials and Methods: Between October 2011 to December 2012, tissue from the bladder tumor of 21 patients (cases tumor, CT), normal mucosa (case control, CC) of the same patients (n-21) and normal bladder mucosa from 10 HCs were obtained. miRNAs of angiogenesis, endothelial mesenchymal transition and apoptosis were quantified using stem-loop RT Taq Man polymerase chain reaction. Statistical analysis was performed using the Chi square and independent sample T tests by using SPSS version 16. Results: The mean age of the patients and controls were 55.41 ± 11.03 and 52.14 ± 13.04 years. miR-21, miR-205, miR-126, miR-10b and miR-200a were highly expressed in CT (P < 0.027, <0.048, <0.025, <0.029 and < 0.005) as compared with HC. Expression of miR-21 and miR-129 were both correlated with grade and stage (P = 0.001 and < 0.009, respectively) and the level of expression was different in the same grade of non-muscle invasive tumors. The fold change of miR129, miR205 and miR200a was significantly higher in the normal-looking mucosa of bladder tumor patients than the HC (P < 0.005). Conclusion: Expression of miR129, miR205 and miR200a in the normal-looking mucosa of bladder cancer patients was significantly higher than the normal mucosa of a HC. This may help in predicting recurrence and formulating the follow-up strategy.http://www.indianjurol.com/article.asp?issn=0970-1591;year=2016;volume=32;issue=2;spage=149;epage=153;aulast=MitashBladder cancermicro-RNAmuscle invasivenon-muscle invasive |
| spellingShingle | Nilay Mitash Shalini Agnihotri Balraj Mittal Swasti Tiwari Anil Mandhani Molecular cystoscopy: Micro-RNAs could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladder Indian Journal of Urology Bladder cancer micro-RNA muscle invasive non-muscle invasive |
| title | Molecular cystoscopy: Micro-RNAs could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladder |
| title_full | Molecular cystoscopy: Micro-RNAs could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladder |
| title_fullStr | Molecular cystoscopy: Micro-RNAs could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladder |
| title_full_unstemmed | Molecular cystoscopy: Micro-RNAs could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladder |
| title_short | Molecular cystoscopy: Micro-RNAs could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladder |
| title_sort | molecular cystoscopy micro rnas could be a marker for identifying genotypic changes for transitional cell carcinoma of the urinary bladder |
| topic | Bladder cancer micro-RNA muscle invasive non-muscle invasive |
| url | http://www.indianjurol.com/article.asp?issn=0970-1591;year=2016;volume=32;issue=2;spage=149;epage=153;aulast=Mitash |
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