Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells
Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosome...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Cell Adhesion & Migration |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19336918.2019.1568141 |
| _version_ | 1818966999388127232 |
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| author | Zhiping Yan Guanyue Su Wenbo Gao Jia He Yang Shen Ye Zeng Xiaoheng Liu |
| author_facet | Zhiping Yan Guanyue Su Wenbo Gao Jia He Yang Shen Ye Zeng Xiaoheng Liu |
| author_sort | Zhiping Yan |
| collection | DOAJ |
| description | Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosomes. Inhibition of integrin by Cliengitide (Cli) or inhibition of the microfilaments formation both inhibited the activation of autophagy in HepG2 under FSS. In addition, Cli inhibited the microfilaments formation and expressions of Rac1 and RhoA in HepG2 cells under FSS. Finally, inhibition of autophagy suppressed the cell migration and invasion in HepG2 under FSS. In conclusion, FSS induced autophagy to promote migration and invasion of HepG2 cells via integrin/cytoskeleton pathways. |
| first_indexed | 2024-12-20T13:41:49Z |
| format | Article |
| id | doaj.art-bbb05b102ab748d6a979f1ee5e419656 |
| institution | Directory Open Access Journal |
| issn | 1933-6918 1933-6926 |
| language | English |
| last_indexed | 2024-12-20T13:41:49Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj.art-bbb05b102ab748d6a979f1ee5e4196562022-12-21T19:38:48ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262019-01-0113115216310.1080/19336918.2019.15681411568141Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cellsZhiping Yan0Guanyue Su1Wenbo Gao2Jia He3Yang Shen4Ye Zeng5Xiaoheng Liu6Sichuan UniversitySichuan UniversitySichuan UniversitySichuan UniversitySichuan UniversitySichuan UniversitySichuan UniversityFluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosomes. Inhibition of integrin by Cliengitide (Cli) or inhibition of the microfilaments formation both inhibited the activation of autophagy in HepG2 under FSS. In addition, Cli inhibited the microfilaments formation and expressions of Rac1 and RhoA in HepG2 cells under FSS. Finally, inhibition of autophagy suppressed the cell migration and invasion in HepG2 under FSS. In conclusion, FSS induced autophagy to promote migration and invasion of HepG2 cells via integrin/cytoskeleton pathways.http://dx.doi.org/10.1080/19336918.2019.1568141fluid shear stressautophagyintegrincytoskeletonmetastasis |
| spellingShingle | Zhiping Yan Guanyue Su Wenbo Gao Jia He Yang Shen Ye Zeng Xiaoheng Liu Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells Cell Adhesion & Migration fluid shear stress autophagy integrin cytoskeleton metastasis |
| title | Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells |
| title_full | Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells |
| title_fullStr | Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells |
| title_full_unstemmed | Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells |
| title_short | Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells |
| title_sort | fluid shear stress induces cell migration and invasion via activating autophagy in hepg2 cells |
| topic | fluid shear stress autophagy integrin cytoskeleton metastasis |
| url | http://dx.doi.org/10.1080/19336918.2019.1568141 |
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