In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31
Surgical failures, caused by postoperative infections of bone implants, are commonly met, which cannot be treated precisely with intravenous antibiotics. Photothermal therapy (PTT) and photodynamic therapy (PDT) have attracted widespread attention due to their non-invasive antibacterial effects on t...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-12-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X22000640 |
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| author | Zhen-Yu Zhang Yan-Lin An Xiao-Shi Wang Lan-Yue Cui Shuo-Qi Li Cheng-Bao Liu Yu-Hong Zou Fen Zhang Rong-Chang Zeng |
| author_facet | Zhen-Yu Zhang Yan-Lin An Xiao-Shi Wang Lan-Yue Cui Shuo-Qi Li Cheng-Bao Liu Yu-Hong Zou Fen Zhang Rong-Chang Zeng |
| author_sort | Zhen-Yu Zhang |
| collection | DOAJ |
| description | Surgical failures, caused by postoperative infections of bone implants, are commonly met, which cannot be treated precisely with intravenous antibiotics. Photothermal therapy (PTT) and photodynamic therapy (PDT) have attracted widespread attention due to their non-invasive antibacterial effects on tissues and no bacterial resistance, which may be an excellent approach to solve infections related to bone implants for biodegradable magnesium alloys. Herein, a sodium copper chlorophyllin (SCC) with a porphyrin ring induced Ca–P coating was prepared on AZ31 magnesium alloy via layer-by-layer (LbL) assembly. The morphology and composition of the samples were characterized through field emission scanning electron microscope (FE-SEM) with affiliated energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and Fourier infrared spectrometer (FTIR) and X-ray photoelectron spectrometer (XPS) as well. Potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and hydrogen evolution experiments were employed to evaluate the corrosion behavior of the samples. Atomic absorption spectrophotometer was used to measure Cu elemental content of different immersion periods. Cytocompatibility and antibacterial performance of the coatings were probed using in vitro cytotoxicity tests (MTT assay), live/dead cell staining and plate counting method. The results showed that the obtained (Ca–P/SCC)10 coating exhibited good corrosion resistance, antimicrobial activity (especially under 808 nm irradiation) and biocompatibility. The antibacterial rates for E. coli and S. aureus were 99.9% and 99.8%, respectively; and the photothermal conversion efficiency was as high as 42.1%. Triple antibacterial mechanisms including photodynamic, photothermal reactions and copper-ions release were proposed. This coating exhibited a promising application for biodegradable magnesium alloys. |
| first_indexed | 2024-04-12T06:09:47Z |
| format | Article |
| id | doaj.art-bbb6635a42f14cb88335f5d4231b4a52 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:35:59Z |
| publishDate | 2022-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-bbb6635a42f14cb88335f5d4231b4a522024-04-16T16:56:15ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2022-12-0118284299In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31Zhen-Yu Zhang0Yan-Lin An1Xiao-Shi Wang2Lan-Yue Cui3Shuo-Qi Li4Cheng-Bao Liu5Yu-Hong Zou6Fen Zhang7Rong-Chang Zeng8College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, ChinaCollege of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, ChinaCollege of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, ChinaCollege of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, China; Corresponding author.College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, ChinaCollege of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, ChinaCollege of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, 266590, ChinaCollege of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, ChinaCollege of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, China; School of Materials Science and Engineering, Zhengzhou University, Zhengzhou, 450002, ChinaSurgical failures, caused by postoperative infections of bone implants, are commonly met, which cannot be treated precisely with intravenous antibiotics. Photothermal therapy (PTT) and photodynamic therapy (PDT) have attracted widespread attention due to their non-invasive antibacterial effects on tissues and no bacterial resistance, which may be an excellent approach to solve infections related to bone implants for biodegradable magnesium alloys. Herein, a sodium copper chlorophyllin (SCC) with a porphyrin ring induced Ca–P coating was prepared on AZ31 magnesium alloy via layer-by-layer (LbL) assembly. The morphology and composition of the samples were characterized through field emission scanning electron microscope (FE-SEM) with affiliated energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and Fourier infrared spectrometer (FTIR) and X-ray photoelectron spectrometer (XPS) as well. Potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and hydrogen evolution experiments were employed to evaluate the corrosion behavior of the samples. Atomic absorption spectrophotometer was used to measure Cu elemental content of different immersion periods. Cytocompatibility and antibacterial performance of the coatings were probed using in vitro cytotoxicity tests (MTT assay), live/dead cell staining and plate counting method. The results showed that the obtained (Ca–P/SCC)10 coating exhibited good corrosion resistance, antimicrobial activity (especially under 808 nm irradiation) and biocompatibility. The antibacterial rates for E. coli and S. aureus were 99.9% and 99.8%, respectively; and the photothermal conversion efficiency was as high as 42.1%. Triple antibacterial mechanisms including photodynamic, photothermal reactions and copper-ions release were proposed. This coating exhibited a promising application for biodegradable magnesium alloys.http://www.sciencedirect.com/science/article/pii/S2452199X22000640Magnesium alloyCoatingDegradable biomaterialBiocompatibilityAntibacterial activity |
| spellingShingle | Zhen-Yu Zhang Yan-Lin An Xiao-Shi Wang Lan-Yue Cui Shuo-Qi Li Cheng-Bao Liu Yu-Hong Zou Fen Zhang Rong-Chang Zeng In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31 Bioactive Materials Magnesium alloy Coating Degradable biomaterial Biocompatibility Antibacterial activity |
| title | In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31 |
| title_full | In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31 |
| title_fullStr | In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31 |
| title_full_unstemmed | In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31 |
| title_short | In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31 |
| title_sort | in vitro degradation photo dynamic and thermal antibacterial activities of cu bearing chlorophyllin induced ca p coating on magnesium alloy az31 |
| topic | Magnesium alloy Coating Degradable biomaterial Biocompatibility Antibacterial activity |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X22000640 |
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