Role of Oct4 in the early embryo development
Oct4 is a key component of the pluripotency regulatory network, and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells (ESCs) or their differentiation into trophoblast. Oct4 of maternal origin is postulated to play critical role...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2014-01-01
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| Series: | Cell Regeneration |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2045976917300226 |
| _version_ | 1818539531963465728 |
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| author | Guangming Wu Hans R Schöler |
| author_facet | Guangming Wu Hans R Schöler |
| author_sort | Guangming Wu |
| collection | DOAJ |
| description | Oct4 is a key component of the pluripotency regulatory network, and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells (ESCs) or their differentiation into trophoblast. Oct4 of maternal origin is postulated to play critical role in defining totipotency and inducing pluripotency during embryonic development. However, the genetic elimination of maternal Oct4 using a Cre-lox approach in mouse revealed that the establishment of totipotency in maternal Oct4–depleted embryos was not affected, and that these embryos could complete full-term development without any obvious defect. These results indicate that Oct4 is not essential for the initiation of pluripotency, in contrast to its critical role in maintaining pluripotency. This conclusion is further supported by the formation of Oct4-GFP– and Nanog- expressing inner cell masses (ICMs) in embryos with complete inactivation of both maternal and zygotic Oct4 expression and the reprogramming of fibroblasts into fully pluripotent cells by Oct4-deficient oocytes. |
| first_indexed | 2024-12-11T21:43:17Z |
| format | Article |
| id | doaj.art-bbb67f44a87649da94695ba75f60a8c8 |
| institution | Directory Open Access Journal |
| issn | 2045-9769 |
| language | English |
| last_indexed | 2024-12-11T21:43:17Z |
| publishDate | 2014-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Cell Regeneration |
| spelling | doaj.art-bbb67f44a87649da94695ba75f60a8c82022-12-22T00:49:45ZengSpringerOpenCell Regeneration2045-97692014-01-013110.1186/2045-9769-3-7Role of Oct4 in the early embryo developmentGuangming Wu0Hans R Schöler1Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, GermanyDepartment of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, GermanyOct4 is a key component of the pluripotency regulatory network, and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells (ESCs) or their differentiation into trophoblast. Oct4 of maternal origin is postulated to play critical role in defining totipotency and inducing pluripotency during embryonic development. However, the genetic elimination of maternal Oct4 using a Cre-lox approach in mouse revealed that the establishment of totipotency in maternal Oct4–depleted embryos was not affected, and that these embryos could complete full-term development without any obvious defect. These results indicate that Oct4 is not essential for the initiation of pluripotency, in contrast to its critical role in maintaining pluripotency. This conclusion is further supported by the formation of Oct4-GFP– and Nanog- expressing inner cell masses (ICMs) in embryos with complete inactivation of both maternal and zygotic Oct4 expression and the reprogramming of fibroblasts into fully pluripotent cells by Oct4-deficient oocytes.http://www.sciencedirect.com/science/article/pii/S2045976917300226Oct4Oct4BTotipotencyPluripotencyEmbryoDevelopment |
| spellingShingle | Guangming Wu Hans R Schöler Role of Oct4 in the early embryo development Cell Regeneration Oct4 Oct4B Totipotency Pluripotency Embryo Development |
| title | Role of Oct4 in the early embryo development |
| title_full | Role of Oct4 in the early embryo development |
| title_fullStr | Role of Oct4 in the early embryo development |
| title_full_unstemmed | Role of Oct4 in the early embryo development |
| title_short | Role of Oct4 in the early embryo development |
| title_sort | role of oct4 in the early embryo development |
| topic | Oct4 Oct4B Totipotency Pluripotency Embryo Development |
| url | http://www.sciencedirect.com/science/article/pii/S2045976917300226 |
| work_keys_str_mv | AT guangmingwu roleofoct4intheearlyembryodevelopment AT hansrscholer roleofoct4intheearlyembryodevelopment |