The effects of long-term leptin administration on morphometrical changes of mice testicular tissue
Objective(s):Leptin is a novel and interesting hormone for anyone trying to lose weight, but its effects on male gonad structure in longitudinal study is unknown. The present study was designed to explore morphometrical changes of mouse testicular tissue after long-term administration of leptin. Mat...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2015-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_6269_c488a4b5c45f341be0b4793011aaed26.html |
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| author | Mohammad-Reza Esmaili-Nejad Homayoon Babaei Reza Kheirandish |
| author_facet | Mohammad-Reza Esmaili-Nejad Homayoon Babaei Reza Kheirandish |
| author_sort | Mohammad-Reza Esmaili-Nejad |
| collection | DOAJ |
| description | Objective(s):Leptin is a novel and interesting hormone for anyone trying to lose weight, but its effects on male gonad structure in longitudinal study is unknown. The present study was designed to explore morphometrical changes of mouse testicular tissue after long-term administration of leptin. Materials and Methods:Thirty healthy mature male mice were randomly assigned to either control (n=15) or treatment (n=15) groups. Leptin was intraperitoneally injected to the treatment group (0.1 µg/100 µl of physiological saline) once a day for 30 consecutive days, and control animals received normal saline with the same volume and route. Five mice from each experimental group were sacrificed at 15, 30 and 60 days after the beginning of treatments. Left testes were removed, weighted and then fixed in 10% buffered formalin, and stained with hematoxylin and eosine for morphometrical assays. Results:Except for sertoli cell nucleus diameter, which was affected from 30th day, evaluation of other morphometrical parameters such as Johnsen’s score, meiotic index, spermatogenesis, epithelial height, seminiferous tubules diameter and spermatogonial nucleus diameter revealed significant decrease from 15th day after leptin administration compare to those of the control group (P |
| first_indexed | 2024-12-23T04:09:44Z |
| format | Article |
| id | doaj.art-bbba5f50e4f3416d90693f6cad940c55 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-23T04:09:44Z |
| publishDate | 2015-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-bbba5f50e4f3416d90693f6cad940c552022-12-21T18:00:33ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-12-011812117611826269The effects of long-term leptin administration on morphometrical changes of mice testicular tissueMohammad-Reza Esmaili-Nejad0Homayoon Babaei1Reza Kheirandish2Graduated Student of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, IranObjective(s):Leptin is a novel and interesting hormone for anyone trying to lose weight, but its effects on male gonad structure in longitudinal study is unknown. The present study was designed to explore morphometrical changes of mouse testicular tissue after long-term administration of leptin. Materials and Methods:Thirty healthy mature male mice were randomly assigned to either control (n=15) or treatment (n=15) groups. Leptin was intraperitoneally injected to the treatment group (0.1 µg/100 µl of physiological saline) once a day for 30 consecutive days, and control animals received normal saline with the same volume and route. Five mice from each experimental group were sacrificed at 15, 30 and 60 days after the beginning of treatments. Left testes were removed, weighted and then fixed in 10% buffered formalin, and stained with hematoxylin and eosine for morphometrical assays. Results:Except for sertoli cell nucleus diameter, which was affected from 30th day, evaluation of other morphometrical parameters such as Johnsen’s score, meiotic index, spermatogenesis, epithelial height, seminiferous tubules diameter and spermatogonial nucleus diameter revealed significant decrease from 15th day after leptin administration compare to those of the control group (Phttp://ijbms.mums.ac.ir/pdf_6269_c488a4b5c45f341be0b4793011aaed26.htmlLeptinMeiotic indexSertoli cellSpermatogenesisSpermatogonia cell |
| spellingShingle | Mohammad-Reza Esmaili-Nejad Homayoon Babaei Reza Kheirandish The effects of long-term leptin administration on morphometrical changes of mice testicular tissue Iranian Journal of Basic Medical Sciences Leptin Meiotic index Sertoli cell Spermatogenesis Spermatogonia cell |
| title | The effects of long-term leptin administration on morphometrical changes of mice testicular tissue |
| title_full | The effects of long-term leptin administration on morphometrical changes of mice testicular tissue |
| title_fullStr | The effects of long-term leptin administration on morphometrical changes of mice testicular tissue |
| title_full_unstemmed | The effects of long-term leptin administration on morphometrical changes of mice testicular tissue |
| title_short | The effects of long-term leptin administration on morphometrical changes of mice testicular tissue |
| title_sort | effects of long term leptin administration on morphometrical changes of mice testicular tissue |
| topic | Leptin Meiotic index Sertoli cell Spermatogenesis Spermatogonia cell |
| url | http://ijbms.mums.ac.ir/pdf_6269_c488a4b5c45f341be0b4793011aaed26.html |
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