Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 Induction
Lipid-laden foam cells were considered to be targets for therapeutic intervention in atherosclerosis. Several studies proposed new approaches to alter both lipid accumulation and inflammatory responses in macrophages. Finding anti-inflammatory signals during foam cell formation would provide new val...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2007-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319343038 |
| _version_ | 1819265130195582976 |
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| author | Jian-Li Ma Peng-Yuan Yang Yao-Cheng Rui Ling Lu Hui Kang Jing Zhang |
| author_facet | Jian-Li Ma Peng-Yuan Yang Yao-Cheng Rui Ling Lu Hui Kang Jing Zhang |
| author_sort | Jian-Li Ma |
| collection | DOAJ |
| description | Lipid-laden foam cells were considered to be targets for therapeutic intervention in atherosclerosis. Several studies proposed new approaches to alter both lipid accumulation and inflammatory responses in macrophages. Finding anti-inflammatory signals during foam cell formation would provide new valid targets for anti-atherosclerotic treatment. The aim of the present study was to see whether oxidized low-density lipoprotein (ox-LDL) can active heme oxygenase (HO)-1 expression level in a human monocyte line, U937 cells, associated with the increase of cytokine secretion. We used hemin (HO-1 activator) and zinc protoporphyrin IX (ZnPP IX, HO-1 inhibitor) to determine the effect of HO-1 on the regulation of cytokine expressions. The results showed that hemin can significantly decrease pro-inflammatory cytokines interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels, while enhancing IL-10 production in a dose-dependent manner in U937 foam cells. ZnPP IX did not significantly affect cytokine levels in foam cells. Our present results suggested that HO-1 is an important antiinflammatory therapeutic target through inhibiting pro-inflammatory cytokines and enhancing anti-inflammatory cytokines for the management of atherogenesis. Keywords:: heme oxygenase (HO)-1, hemin, cytokine, macrophage-derived foam cell, atherosclerosis |
| first_indexed | 2024-12-23T20:40:29Z |
| format | Article |
| id | doaj.art-bbbc65933fe04dc89970a222acc8829d |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-23T20:40:29Z |
| publishDate | 2007-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-bbbc65933fe04dc89970a222acc8829d2022-12-21T17:31:56ZengElsevierJournal of Pharmacological Sciences1347-86132007-01-011033261266Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 InductionJian-Li Ma0Peng-Yuan Yang1Yao-Cheng Rui2Ling Lu3Hui Kang4Jing Zhang5Department of Pharmacology, School of Pharmacy, Second Military Medical University, No. 325 GuoHe Road, Shanghai 200433, China; First Affiliated Hospital, General Hospital of PLA, No. 51 FuCheng Road, Beijing 100037, ChinaDepartment of Pharmacology, School of Pharmacy, Second Military Medical University, No. 325 GuoHe Road, Shanghai 200433, ChinaDepartment of Pharmacology, School of Pharmacy, Second Military Medical University, No. 325 GuoHe Road, Shanghai 200433, China; Corresponding author. ruiyc@smmu.edu.cnDepartment of Pharmacology, School of Pharmacy, Second Military Medical University, No. 325 GuoHe Road, Shanghai 200433, ChinaDepartment of Pharmacology, School of Pharmacy, Second Military Medical University, No. 325 GuoHe Road, Shanghai 200433, ChinaDepartment of Pharmacology, School of Pharmacy, Second Military Medical University, No. 325 GuoHe Road, Shanghai 200433, ChinaLipid-laden foam cells were considered to be targets for therapeutic intervention in atherosclerosis. Several studies proposed new approaches to alter both lipid accumulation and inflammatory responses in macrophages. Finding anti-inflammatory signals during foam cell formation would provide new valid targets for anti-atherosclerotic treatment. The aim of the present study was to see whether oxidized low-density lipoprotein (ox-LDL) can active heme oxygenase (HO)-1 expression level in a human monocyte line, U937 cells, associated with the increase of cytokine secretion. We used hemin (HO-1 activator) and zinc protoporphyrin IX (ZnPP IX, HO-1 inhibitor) to determine the effect of HO-1 on the regulation of cytokine expressions. The results showed that hemin can significantly decrease pro-inflammatory cytokines interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels, while enhancing IL-10 production in a dose-dependent manner in U937 foam cells. ZnPP IX did not significantly affect cytokine levels in foam cells. Our present results suggested that HO-1 is an important antiinflammatory therapeutic target through inhibiting pro-inflammatory cytokines and enhancing anti-inflammatory cytokines for the management of atherogenesis. Keywords:: heme oxygenase (HO)-1, hemin, cytokine, macrophage-derived foam cell, atherosclerosishttp://www.sciencedirect.com/science/article/pii/S1347861319343038 |
| spellingShingle | Jian-Li Ma Peng-Yuan Yang Yao-Cheng Rui Ling Lu Hui Kang Jing Zhang Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 Induction Journal of Pharmacological Sciences |
| title | Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 Induction |
| title_full | Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 Induction |
| title_fullStr | Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 Induction |
| title_full_unstemmed | Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 Induction |
| title_short | Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 Induction |
| title_sort | hemin modulates cytokine expressions in macrophage derived foam cells via heme oxygenase 1 induction |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319343038 |
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