Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria
Conventionally, cultivations of Nepenthes are conducted by using seeds, cutting, and filial separation. However, there are many obstacles come both from time and technical aspect. In vitro culture is an alternative way for cultivating N. ampullaria (Jack,). One of a technique of in vitro culture is...
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| Format: | Article |
| Language: | English |
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Universitas Negeri Semarang
2018-12-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/15718 |
| _version_ | 1819260999534903296 |
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| author | Iman Budisantoso Mona Indriani Kamsinah Kamsinah |
| author_facet | Iman Budisantoso Mona Indriani Kamsinah Kamsinah |
| author_sort | Iman Budisantoso |
| collection | DOAJ |
| description | Conventionally, cultivations of Nepenthes are conducted by using seeds, cutting, and filial separation. However, there are many obstacles come both from time and technical aspect. In vitro culture is an alternative way for cultivating N. ampullaria (Jack,). One of a technique of in vitro culture is micro cutting. BAP (6-benzyl amino purine) growth regulator could be added to optimize the growth of N. ampullaria microcutting. The purpose of this research was to determine the effect of BAP on the growth of Nepenthes microcutting. This research was done experimentally using Completely Randomized Design (CRD). BAP treatment consisted of 5 concentrations: 0; 0.5; 1; 1.5; 2 (ppm), each treatment were multiplied 4 times. The parameters observed were: a time of bud initiation, time of root initiation, total of leaves, total of new buds, total of roots, length of leave, length of root, and height of bud. The data obtained were analyzed with ANOVA (Analisis of Variance) and continued with 5% and 1% LSD (Least Significant Different) test. The result showed that addition of BAP affected the growth of N. ampullaria microcutting in total leaves, length of leave, and total of buds. LSD test proved that 0.57 ppm of BAP was optimal concentration to increase total buds, whit the value reached of 3.86. Here, we found that BAP can be utilized to enhanche N. ampullaria growth on in vitro culture. The benefit of this study is to conserve N. ampullaria in vitro using BAP at 0.57 ppm. |
| first_indexed | 2024-12-23T19:34:50Z |
| format | Article |
| id | doaj.art-bbbde7e4b4bc4f05ac1d7209f0bc0970 |
| institution | Directory Open Access Journal |
| issn | 2085-191X 2338-7610 |
| language | English |
| last_indexed | 2024-12-23T19:34:50Z |
| publishDate | 2018-12-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj.art-bbbde7e4b4bc4f05ac1d7209f0bc09702022-12-21T17:33:48ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102018-12-0110367868310.15294/biosaintifika.v10i3.157188162Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullariaIman Budisantoso0Mona Indriani1Kamsinah Kamsinah2Faculty of Biology, Universitas Jenderal SoedirmanFaculty of Biology, Universitas Jenderal SoedirmanFaculty of Biology, Universitas Jenderal SoedirmanConventionally, cultivations of Nepenthes are conducted by using seeds, cutting, and filial separation. However, there are many obstacles come both from time and technical aspect. In vitro culture is an alternative way for cultivating N. ampullaria (Jack,). One of a technique of in vitro culture is micro cutting. BAP (6-benzyl amino purine) growth regulator could be added to optimize the growth of N. ampullaria microcutting. The purpose of this research was to determine the effect of BAP on the growth of Nepenthes microcutting. This research was done experimentally using Completely Randomized Design (CRD). BAP treatment consisted of 5 concentrations: 0; 0.5; 1; 1.5; 2 (ppm), each treatment were multiplied 4 times. The parameters observed were: a time of bud initiation, time of root initiation, total of leaves, total of new buds, total of roots, length of leave, length of root, and height of bud. The data obtained were analyzed with ANOVA (Analisis of Variance) and continued with 5% and 1% LSD (Least Significant Different) test. The result showed that addition of BAP affected the growth of N. ampullaria microcutting in total leaves, length of leave, and total of buds. LSD test proved that 0.57 ppm of BAP was optimal concentration to increase total buds, whit the value reached of 3.86. Here, we found that BAP can be utilized to enhanche N. ampullaria growth on in vitro culture. The benefit of this study is to conserve N. ampullaria in vitro using BAP at 0.57 ppm.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/15718nepenthes ampullaria (jack)in vitro culturemicro cuttingbap |
| spellingShingle | Iman Budisantoso Mona Indriani Kamsinah Kamsinah Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria Biosaintifika: Journal of Biology & Biology Education nepenthes ampullaria (jack) in vitro culture micro cutting bap |
| title | Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria |
| title_full | Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria |
| title_fullStr | Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria |
| title_full_unstemmed | Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria |
| title_short | Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria |
| title_sort | effect of bap 6 benzyl amino purine concentration on growth micro cutting of nepenthes ampullaria |
| topic | nepenthes ampullaria (jack) in vitro culture micro cutting bap |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/15718 |
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