Molecular Characterization of Mitogenome of <i>Cacopsylla picta</i> and <i>Cacopsylla melanoneura</i>, Two Vector Species of ‘<i>Candidatus</i> Phytoplasma mali’
The mitochondrial genomes of two vector psyllids of the ‘<i>Candidatus</i> Phytoplasma mali’, <i>Cacopsylla picta</i> and <i>C. melanoneura</i>, were sequenced using high-throughput sequencing on the Illumina platform. The main objective of the study was to descri...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/9/2210 |
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| author | Dana Šafářová Erika Zrníková Kateřina Holušová Jana Ouředníčková Martin Starý Milan Navrátil |
| author_facet | Dana Šafářová Erika Zrníková Kateřina Holušová Jana Ouředníčková Martin Starý Milan Navrátil |
| author_sort | Dana Šafářová |
| collection | DOAJ |
| description | The mitochondrial genomes of two vector psyllids of the ‘<i>Candidatus</i> Phytoplasma mali’, <i>Cacopsylla picta</i> and <i>C. melanoneura</i>, were sequenced using high-throughput sequencing on the Illumina platform. The main objective of the study was to describe their mitogenome and characterize their genetic variability and the potential changes in the context of the observed global warming. The four complete sequences for <i>C. picta</i>, 14,801 bp and 14,802 bp in length, two complete and one partial sequence for <i>C. melanoneura</i>, ranging from 14,879 bp to 14,881 bp in length, were obtained for the first time for these European apple psyllids. The detected intraspecies mtDNA identity was highly similar (99.85–99.98%), the identity’s similarity with other <i>Cacopsylla</i> species varied between 79.79 and 86.64%. The mitogenomes showed a typical mitochondrial DNA structure with 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes; the presence of CGGA motif in the ND1-trnS2 junction was detected in both species. Phylogenetic analysis placed both species in close relationship with <i>C. burckhardti</i> within the Cacopsylla clade-I O group. The analysis of complete mitogenomes and of partial COI sequences of fifty-two <i>Cacopsylla</i> individuals showed a high homogeneity of genotypes over 15 years and among the different localities in the Czech Republic. |
| first_indexed | 2024-03-10T23:08:50Z |
| format | Article |
| id | doaj.art-bbc060a56c4c49f38927828266bb13b8 |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T23:08:50Z |
| publishDate | 2023-08-01 |
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| series | Agronomy |
| spelling | doaj.art-bbc060a56c4c49f38927828266bb13b82023-11-19T09:09:07ZengMDPI AGAgronomy2073-43952023-08-01139221010.3390/agronomy13092210Molecular Characterization of Mitogenome of <i>Cacopsylla picta</i> and <i>Cacopsylla melanoneura</i>, Two Vector Species of ‘<i>Candidatus</i> Phytoplasma mali’Dana Šafářová0Erika Zrníková1Kateřina Holušová2Jana Ouředníčková3Martin Starý4Milan Navrátil5Department of Cell Biology and Genetics, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, 779 00 Olomouc, Czech RepublicDepartment of Cell Biology and Genetics, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, 779 00 Olomouc, Czech RepublicInstitute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 779 00 Olomouc, Czech RepublicResearch and Breeding Institute of Pomology Holovousy Ltd., Holovousy 129, 508 01 Holovousy, Czech RepublicDepartment of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 602 00 Brno, Czech RepublicDepartment of Cell Biology and Genetics, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, 779 00 Olomouc, Czech RepublicThe mitochondrial genomes of two vector psyllids of the ‘<i>Candidatus</i> Phytoplasma mali’, <i>Cacopsylla picta</i> and <i>C. melanoneura</i>, were sequenced using high-throughput sequencing on the Illumina platform. The main objective of the study was to describe their mitogenome and characterize their genetic variability and the potential changes in the context of the observed global warming. The four complete sequences for <i>C. picta</i>, 14,801 bp and 14,802 bp in length, two complete and one partial sequence for <i>C. melanoneura</i>, ranging from 14,879 bp to 14,881 bp in length, were obtained for the first time for these European apple psyllids. The detected intraspecies mtDNA identity was highly similar (99.85–99.98%), the identity’s similarity with other <i>Cacopsylla</i> species varied between 79.79 and 86.64%. The mitogenomes showed a typical mitochondrial DNA structure with 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes; the presence of CGGA motif in the ND1-trnS2 junction was detected in both species. Phylogenetic analysis placed both species in close relationship with <i>C. burckhardti</i> within the Cacopsylla clade-I O group. The analysis of complete mitogenomes and of partial COI sequences of fifty-two <i>Cacopsylla</i> individuals showed a high homogeneity of genotypes over 15 years and among the different localities in the Czech Republic.https://www.mdpi.com/2073-4395/13/9/2210apple pestshigh-throughput sequencing complete mitochondrial DNAphylogenetic analysisfruit tree phytoplasma |
| spellingShingle | Dana Šafářová Erika Zrníková Kateřina Holušová Jana Ouředníčková Martin Starý Milan Navrátil Molecular Characterization of Mitogenome of <i>Cacopsylla picta</i> and <i>Cacopsylla melanoneura</i>, Two Vector Species of ‘<i>Candidatus</i> Phytoplasma mali’ Agronomy apple pests high-throughput sequencing complete mitochondrial DNA phylogenetic analysis fruit tree phytoplasma |
| title | Molecular Characterization of Mitogenome of <i>Cacopsylla picta</i> and <i>Cacopsylla melanoneura</i>, Two Vector Species of ‘<i>Candidatus</i> Phytoplasma mali’ |
| title_full | Molecular Characterization of Mitogenome of <i>Cacopsylla picta</i> and <i>Cacopsylla melanoneura</i>, Two Vector Species of ‘<i>Candidatus</i> Phytoplasma mali’ |
| title_fullStr | Molecular Characterization of Mitogenome of <i>Cacopsylla picta</i> and <i>Cacopsylla melanoneura</i>, Two Vector Species of ‘<i>Candidatus</i> Phytoplasma mali’ |
| title_full_unstemmed | Molecular Characterization of Mitogenome of <i>Cacopsylla picta</i> and <i>Cacopsylla melanoneura</i>, Two Vector Species of ‘<i>Candidatus</i> Phytoplasma mali’ |
| title_short | Molecular Characterization of Mitogenome of <i>Cacopsylla picta</i> and <i>Cacopsylla melanoneura</i>, Two Vector Species of ‘<i>Candidatus</i> Phytoplasma mali’ |
| title_sort | molecular characterization of mitogenome of i cacopsylla picta i and i cacopsylla melanoneura i two vector species of i candidatus i phytoplasma mali |
| topic | apple pests high-throughput sequencing complete mitochondrial DNA phylogenetic analysis fruit tree phytoplasma |
| url | https://www.mdpi.com/2073-4395/13/9/2210 |
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