Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium
Abstract Citrus limetta peels (CLP), a waste material generated by juice industries, has scarcely been reported for the production of yeast enzymes. The study was conducted to obtain a multienzyme preparation from a yeast consortium under solid-state fermentation of CLP. The substrate, CLP, was pret...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-11-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-019-0278-0 |
| _version_ | 1818532258576859136 |
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| author | Maria Shariq Muhammad Sohail |
| author_facet | Maria Shariq Muhammad Sohail |
| author_sort | Maria Shariq |
| collection | DOAJ |
| description | Abstract Citrus limetta peels (CLP), a waste material generated by juice industries, has scarcely been reported for the production of yeast enzymes. The study was conducted to obtain a multienzyme preparation from a yeast consortium under solid-state fermentation of CLP. The substrate, CLP, was pretreated using either acid or alkali, and factors affecting production of multienzyme were studied by generating two separate Plackett–Burman designs. Since, alkali-pretreated CLP yielded higher titers; therefore, significant factors affecting multienzyme preparation using this substrate were optimized by employing Box–Behnken design. The analysis revealed that under optimized conditions, i.e., cultivation of yeast strains for 72 h to alkali-pretreated CLP moistened with mineral salt medium having pH 5 yielded more than 10 IU mL−1 of cellulase, xylanase, and amylase. The multienzyme was studied for its application to saccharify fruit and non-fruit wastes and for orange juice clarification. The data showed that the enzyme preparation could release 3.03 mg L−1 h−1 of reducing sugars from various crude substrates and was able to reduce turbidity of orange juice by 11% with substantial decrease in viscosity and acidity. Hence, CLP appeared as a promising substrate to produce multienzyme preparation from yeast consortium. |
| first_indexed | 2024-12-11T17:43:09Z |
| format | Article |
| id | doaj.art-bbc6a1c37b1a446681479081db4e301b |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-11T17:43:09Z |
| publishDate | 2019-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-bbc6a1c37b1a446681479081db4e301b2022-12-22T00:56:28ZengSpringerOpenBioresources and Bioprocessing2197-43652019-11-016111510.1186/s40643-019-0278-0Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortiumMaria Shariq0Muhammad Sohail1Department of Microbiology, University of KarachiDepartment of Microbiology, University of KarachiAbstract Citrus limetta peels (CLP), a waste material generated by juice industries, has scarcely been reported for the production of yeast enzymes. The study was conducted to obtain a multienzyme preparation from a yeast consortium under solid-state fermentation of CLP. The substrate, CLP, was pretreated using either acid or alkali, and factors affecting production of multienzyme were studied by generating two separate Plackett–Burman designs. Since, alkali-pretreated CLP yielded higher titers; therefore, significant factors affecting multienzyme preparation using this substrate were optimized by employing Box–Behnken design. The analysis revealed that under optimized conditions, i.e., cultivation of yeast strains for 72 h to alkali-pretreated CLP moistened with mineral salt medium having pH 5 yielded more than 10 IU mL−1 of cellulase, xylanase, and amylase. The multienzyme was studied for its application to saccharify fruit and non-fruit wastes and for orange juice clarification. The data showed that the enzyme preparation could release 3.03 mg L−1 h−1 of reducing sugars from various crude substrates and was able to reduce turbidity of orange juice by 11% with substantial decrease in viscosity and acidity. Hence, CLP appeared as a promising substrate to produce multienzyme preparation from yeast consortium.http://link.springer.com/article/10.1186/s40643-019-0278-0Box–Behnken designCitrus limettaMultienzyme preparationPlackett–Burman designSaccharificationYeast consortium |
| spellingShingle | Maria Shariq Muhammad Sohail Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium Bioresources and Bioprocessing Box–Behnken design Citrus limetta Multienzyme preparation Plackett–Burman design Saccharification Yeast consortium |
| title | Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium |
| title_full | Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium |
| title_fullStr | Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium |
| title_full_unstemmed | Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium |
| title_short | Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium |
| title_sort | citrus limetta peels a promising substrate for the production of multienzyme preparation from a yeast consortium |
| topic | Box–Behnken design Citrus limetta Multienzyme preparation Plackett–Burman design Saccharification Yeast consortium |
| url | http://link.springer.com/article/10.1186/s40643-019-0278-0 |
| work_keys_str_mv | AT mariashariq citruslimettapeelsapromisingsubstratefortheproductionofmultienzymepreparationfromayeastconsortium AT muhammadsohail citruslimettapeelsapromisingsubstratefortheproductionofmultienzymepreparationfromayeastconsortium |