Protocol for in vivo dual-color fiber photometry in the mouse thalamus
Summary: In vivo calcium imaging of neural activity is an indispensable approach for understanding the mechanisms and functions of neural system. Development of advanced imaging tools and various genetically encoded calcium indicators allows us to simultaneously record the activity of different neur...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724000960 |
| _version_ | 1827320461709541376 |
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| author | Almas Serikov Iryna Martsishevska Wooyeon Shin Jeongjin Kim |
| author_facet | Almas Serikov Iryna Martsishevska Wooyeon Shin Jeongjin Kim |
| author_sort | Almas Serikov |
| collection | DOAJ |
| description | Summary: In vivo calcium imaging of neural activity is an indispensable approach for understanding the mechanisms and functions of neural system. Development of advanced imaging tools and various genetically encoded calcium indicators allows us to simultaneously record the activity of different neural populations. Here, we present a protocol for acquiring neural activity of two discrete neural populations in mice using dual-color fiber photometry. We describe steps for injecting viral constructs and implanting the fiber optic through stereotaxic surgery, calcium signal acquisition, and data analysis. We also describe the incorporation of electroencephalogram and electromyography recordings with dual-color fiber photometry analysis.For complete details on the use and execution of this protocol, please refer to Shin et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-25T00:46:44Z |
| format | Article |
| id | doaj.art-bbc85249c5294e31857be7e40da6bea8 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-25T00:46:44Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-bbc85249c5294e31857be7e40da6bea82024-03-12T04:16:51ZengElsevierSTAR Protocols2666-16672024-06-0152102931Protocol for in vivo dual-color fiber photometry in the mouse thalamusAlmas Serikov0Iryna Martsishevska1Wooyeon Shin2Jeongjin Kim3Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST), Daejeon, Republic of KoreaBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST), Daejeon, Republic of KoreaBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Program of Brain and Cognitive Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of KoreaBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST), Daejeon, Republic of Korea; Corresponding authorSummary: In vivo calcium imaging of neural activity is an indispensable approach for understanding the mechanisms and functions of neural system. Development of advanced imaging tools and various genetically encoded calcium indicators allows us to simultaneously record the activity of different neural populations. Here, we present a protocol for acquiring neural activity of two discrete neural populations in mice using dual-color fiber photometry. We describe steps for injecting viral constructs and implanting the fiber optic through stereotaxic surgery, calcium signal acquisition, and data analysis. We also describe the incorporation of electroencephalogram and electromyography recordings with dual-color fiber photometry analysis.For complete details on the use and execution of this protocol, please refer to Shin et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724000960NeuroscienceBehavior |
| spellingShingle | Almas Serikov Iryna Martsishevska Wooyeon Shin Jeongjin Kim Protocol for in vivo dual-color fiber photometry in the mouse thalamus STAR Protocols Neuroscience Behavior |
| title | Protocol for in vivo dual-color fiber photometry in the mouse thalamus |
| title_full | Protocol for in vivo dual-color fiber photometry in the mouse thalamus |
| title_fullStr | Protocol for in vivo dual-color fiber photometry in the mouse thalamus |
| title_full_unstemmed | Protocol for in vivo dual-color fiber photometry in the mouse thalamus |
| title_short | Protocol for in vivo dual-color fiber photometry in the mouse thalamus |
| title_sort | protocol for in vivo dual color fiber photometry in the mouse thalamus |
| topic | Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724000960 |
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