Enzyme-Assisted Extraction for the Recovery of Food-Grade Chlorophyll-Based Green Colorant
The aim of the study was to develop a biotechnological approach for the green recovery of chlorophyll from spinach, to be used as a natural food colorant. The plant matrix was characterized in terms of cell wall polysaccharide composition, and a tailored enzymatic mix based on cellulase (40%) xylana...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/18/3440 |
| _version_ | 1797580104102051840 |
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| author | Caterina Mazzocchi Ilaria Benucci Claudio Lombardelli Marco Esti |
| author_facet | Caterina Mazzocchi Ilaria Benucci Claudio Lombardelli Marco Esti |
| author_sort | Caterina Mazzocchi |
| collection | DOAJ |
| description | The aim of the study was to develop a biotechnological approach for the green recovery of chlorophyll from spinach, to be used as a natural food colorant. The plant matrix was characterized in terms of cell wall polysaccharide composition, and a tailored enzymatic mix based on cellulase (40%) xylanase (41%) and polygalacturonase (19%) was formulated. The process variables (temperature (°C), time (h), enzyme mix dose (U/g), zinc concentration (ppm), and buffer/substrate ratio (B/S)) and their interactions were studied by response surface methodology. The overlay plot made it possible to identify the process conditions (T: 25 °C, Zn: 150 ppm e B/S: 17.5, t: <2 h and enzyme mix dose between 12 and 45 U/g) to maximize the amount of chlorophyll, and concurrently, the quality of the green color of the extract. Finally, the novel colorant was applied in the production of a real food. |
| first_indexed | 2024-03-10T22:45:38Z |
| format | Article |
| id | doaj.art-bbc8720a2fc84da6b17837ee37a7d20a |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T22:45:38Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-bbc8720a2fc84da6b17837ee37a7d20a2023-11-19T10:43:15ZengMDPI AGFoods2304-81582023-09-011218344010.3390/foods12183440Enzyme-Assisted Extraction for the Recovery of Food-Grade Chlorophyll-Based Green ColorantCaterina Mazzocchi0Ilaria Benucci1Claudio Lombardelli2Marco Esti3Department of Agriculture and Forest Sciences (DAFNE), Tuscia University, Via S. Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Agriculture and Forest Sciences (DAFNE), Tuscia University, Via S. Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Agriculture and Forest Sciences (DAFNE), Tuscia University, Via S. Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Agriculture and Forest Sciences (DAFNE), Tuscia University, Via S. Camillo de Lellis snc, 01100 Viterbo, ItalyThe aim of the study was to develop a biotechnological approach for the green recovery of chlorophyll from spinach, to be used as a natural food colorant. The plant matrix was characterized in terms of cell wall polysaccharide composition, and a tailored enzymatic mix based on cellulase (40%) xylanase (41%) and polygalacturonase (19%) was formulated. The process variables (temperature (°C), time (h), enzyme mix dose (U/g), zinc concentration (ppm), and buffer/substrate ratio (B/S)) and their interactions were studied by response surface methodology. The overlay plot made it possible to identify the process conditions (T: 25 °C, Zn: 150 ppm e B/S: 17.5, t: <2 h and enzyme mix dose between 12 and 45 U/g) to maximize the amount of chlorophyll, and concurrently, the quality of the green color of the extract. Finally, the novel colorant was applied in the production of a real food.https://www.mdpi.com/2304-8158/12/18/3440solvent-free extractioncircular economyzincnatural pigment recoveryresponse surface methodology |
| spellingShingle | Caterina Mazzocchi Ilaria Benucci Claudio Lombardelli Marco Esti Enzyme-Assisted Extraction for the Recovery of Food-Grade Chlorophyll-Based Green Colorant Foods solvent-free extraction circular economy zinc natural pigment recovery response surface methodology |
| title | Enzyme-Assisted Extraction for the Recovery of Food-Grade Chlorophyll-Based Green Colorant |
| title_full | Enzyme-Assisted Extraction for the Recovery of Food-Grade Chlorophyll-Based Green Colorant |
| title_fullStr | Enzyme-Assisted Extraction for the Recovery of Food-Grade Chlorophyll-Based Green Colorant |
| title_full_unstemmed | Enzyme-Assisted Extraction for the Recovery of Food-Grade Chlorophyll-Based Green Colorant |
| title_short | Enzyme-Assisted Extraction for the Recovery of Food-Grade Chlorophyll-Based Green Colorant |
| title_sort | enzyme assisted extraction for the recovery of food grade chlorophyll based green colorant |
| topic | solvent-free extraction circular economy zinc natural pigment recovery response surface methodology |
| url | https://www.mdpi.com/2304-8158/12/18/3440 |
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