Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded with Doxorubicin on Human Hepatoma Cells
Background: Anthracyclines are used to treat different types of cancer including hepatocellular carcinoma. However, they have various side effects such as cardiotoxicity. Designing a drug delivery system which targets retinoic acid receptors in hepatocellular carcinoma can reduce these side effects....
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2013-02-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2398 |
| _version_ | 1797710792502542336 |
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| author | Jaleh Varshosaz Farshid Hassanzadeh Hojat Sadeghi Aliabadi Zahra Alimohammad Ghelichkhan |
| author_facet | Jaleh Varshosaz Farshid Hassanzadeh Hojat Sadeghi Aliabadi Zahra Alimohammad Ghelichkhan |
| author_sort | Jaleh Varshosaz |
| collection | DOAJ |
| description | Background: Anthracyclines are used to treat different types of cancer including hepatocellular carcinoma. However, they have various side effects such as cardiotoxicity. Designing a drug delivery system which targets retinoic acid receptors in hepatocellular carcinoma can reduce these side effects.
Methods: Chitosan/retinoic acid/albumin nanoparticles were prepared using a coacervation method. Nanoparticles which were optimized according to their particle size, zeta potential, polydispersity index, loading efficiency and release of doxorubicin, were used for further tests of cytotoxicity on HepG2 cells [by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay] and cellular uptake tests (by fluorescent microscopy).
Findings: The optimum targeted nanoparticles (with particle size of 286 ± 50.0 nm, zeta potential of 30.5 ± 3.6 mv, polydispersity index of 0.50 ± 0.06, loading efficiency of 43.6 ± 13.5%, and drug release of 56.17 ± 6.00%) in a concentration of 0.5 mg/ml were two to three times more cytotoxic than non-targeted nanoparticles and free drug. Their cellular uptake was also more.
Conclusion: Chitosan/retinoic acid/albumin targeted nanoparticles loaded with doxorubicin could affect HepG2 cells more effectively than non-targeted nanoparticles and free drug.
Keywords: Chitosan/retinoic acid/albumin nanoparticles, Doxorubicin, HepG2, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay |
| first_indexed | 2024-03-12T06:57:24Z |
| format | Article |
| id | doaj.art-bbc98e2d9f8c44468bb96c5a843a79f4 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T06:57:24Z |
| publishDate | 2013-02-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-bbc98e2d9f8c44468bb96c5a843a79f42023-09-02T23:57:58ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-02-0130218220622161231Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded with Doxorubicin on Human Hepatoma CellsJaleh Varshosaz0Farshid Hassanzadeh1Hojat Sadeghi Aliabadi2Zahra Alimohammad Ghelichkhan3Professor, Department of Pharmaceutics, School of Pharmacy AND Novel Drug Delivery Systems Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Medicinal Chemistry, School of Pharmacy AND Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, IranProfessor, Department of Biotechnology, School of Pharmacy AND Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, IranPharm D Student, Department of Pharmaceutics, School of Pharmacy AND Novel Drug Delivery Systems Research Center AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranBackground: Anthracyclines are used to treat different types of cancer including hepatocellular carcinoma. However, they have various side effects such as cardiotoxicity. Designing a drug delivery system which targets retinoic acid receptors in hepatocellular carcinoma can reduce these side effects. Methods: Chitosan/retinoic acid/albumin nanoparticles were prepared using a coacervation method. Nanoparticles which were optimized according to their particle size, zeta potential, polydispersity index, loading efficiency and release of doxorubicin, were used for further tests of cytotoxicity on HepG2 cells [by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay] and cellular uptake tests (by fluorescent microscopy). Findings: The optimum targeted nanoparticles (with particle size of 286 ± 50.0 nm, zeta potential of 30.5 ± 3.6 mv, polydispersity index of 0.50 ± 0.06, loading efficiency of 43.6 ± 13.5%, and drug release of 56.17 ± 6.00%) in a concentration of 0.5 mg/ml were two to three times more cytotoxic than non-targeted nanoparticles and free drug. Their cellular uptake was also more. Conclusion: Chitosan/retinoic acid/albumin targeted nanoparticles loaded with doxorubicin could affect HepG2 cells more effectively than non-targeted nanoparticles and free drug. Keywords: Chitosan/retinoic acid/albumin nanoparticles, Doxorubicin, HepG2, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assayhttp://jims.mui.ac.ir/index.php/jims/article/view/2398 |
| spellingShingle | Jaleh Varshosaz Farshid Hassanzadeh Hojat Sadeghi Aliabadi Zahra Alimohammad Ghelichkhan Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded with Doxorubicin on Human Hepatoma Cells مجله دانشکده پزشکی اصفهان |
| title | Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded with Doxorubicin on Human Hepatoma Cells |
| title_full | Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded with Doxorubicin on Human Hepatoma Cells |
| title_fullStr | Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded with Doxorubicin on Human Hepatoma Cells |
| title_full_unstemmed | Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded with Doxorubicin on Human Hepatoma Cells |
| title_short | Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded with Doxorubicin on Human Hepatoma Cells |
| title_sort | cytotoxic effects of chitosan retinoic acid albumin targeted nanoparticles loaded with doxorubicin on human hepatoma cells |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2398 |
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