Involvement of Dorsal Hippocampal Muscarinic Cholinergic Receptors of CA1 Area on Anxiety Induced by Iron Oxide Nanoparticles in Adult Male Rats
Introduction: Recent findings revealed the biological effects of iron oxide nanoparticles on the central nervous system. Moreover, the brain cholinergic system plays a role in the modulation of anxiety behaviors. Therefore, this study aimed to evaluate the role of dorsal hippocampal muscarinic choli...
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| Format: | Article |
| Language: | fas |
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Ilam University of Medical Sciences
2019-06-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| Subjects: | |
| Online Access: | http://sjimu.medilam.ac.ir/article-1-5001-en.html |
| _version_ | 1818395349880930304 |
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| author | Lotfollah Khajehpour Azam Karimi Mahnaz Kesmati Mozhgan Torabi |
| author_facet | Lotfollah Khajehpour Azam Karimi Mahnaz Kesmati Mozhgan Torabi |
| author_sort | Lotfollah Khajehpour |
| collection | DOAJ |
| description | Introduction: Recent findings revealed the biological effects of iron oxide nanoparticles on the central nervous system. Moreover, the brain cholinergic system plays a role in the modulation of anxiety behaviors. Therefore, this study aimed to evaluate the role of dorsal hippocampal muscarinic cholinergic receptors of the CA1 area on anxiety induced by iron oxide nanoparticles in adult male rats.
Materials & Methods: This experimental study was conducted on adult male Wistar rats with a weight range of 200-250 g. All animals were cannulated in the CA1 area using stereotactic surgery. One week after the recovery the intracerebroventricular injection followed by intraperitoneal (IP) injection after 5 min were administered. Anxiety-like behavior and locomotor activity were performed by elevated plus maze apparatus one week after the injections. The groups were divided into control (saline), iron oxide nanoparticle (5, 7.5 mg/kg,IP), pilocarpine (1,2 µg/rat, intra-hippocampal injection), pilocarpine (1µg/rat, intra-hippocampal injection)+iron oxide nanoparticle (7.5mg/kg, IP) ,and pilocarpine (2µg/rat, intra-hippocampal injection)+iron oxide nanoparticle (7.5mg/kg, IP). Ethics code: EE/96.24.3.88369/SCU.AC.IR
Findings: Iron oxide nanoparticles (7.5 mg/kg) increased the anxiety level, compared to the control group (P<0.05). However, pilocarpine injection (1μg/rat) before iron oxide nanoparticles (7.5 mg/kg) improved the anxiety induced by iron oxide nanoparticle (P<0.05).
Discussion &Conclusions: It seems that probably the anxiogenic effect of iron oxide nanoparticles is mediated through the reduction of dorsal hippocampal muscarinic cholinergic receptor functions in CA1area. |
| first_indexed | 2024-12-14T06:15:42Z |
| format | Article |
| id | doaj.art-bbcb087d37e24a01a2c76fc05da6d783 |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-12-14T06:15:42Z |
| publishDate | 2019-06-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-bbcb087d37e24a01a2c76fc05da6d7832022-12-21T23:14:03ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352019-06-01272169178Involvement of Dorsal Hippocampal Muscarinic Cholinergic Receptors of CA1 Area on Anxiety Induced by Iron Oxide Nanoparticles in Adult Male RatsLotfollah Khajehpour0Azam Karimi1Mahnaz Kesmati2Mozhgan Torabi3 Dept of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Dept of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Dept of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Dept of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Introduction: Recent findings revealed the biological effects of iron oxide nanoparticles on the central nervous system. Moreover, the brain cholinergic system plays a role in the modulation of anxiety behaviors. Therefore, this study aimed to evaluate the role of dorsal hippocampal muscarinic cholinergic receptors of the CA1 area on anxiety induced by iron oxide nanoparticles in adult male rats. Materials & Methods: This experimental study was conducted on adult male Wistar rats with a weight range of 200-250 g. All animals were cannulated in the CA1 area using stereotactic surgery. One week after the recovery the intracerebroventricular injection followed by intraperitoneal (IP) injection after 5 min were administered. Anxiety-like behavior and locomotor activity were performed by elevated plus maze apparatus one week after the injections. The groups were divided into control (saline), iron oxide nanoparticle (5, 7.5 mg/kg,IP), pilocarpine (1,2 µg/rat, intra-hippocampal injection), pilocarpine (1µg/rat, intra-hippocampal injection)+iron oxide nanoparticle (7.5mg/kg, IP) ,and pilocarpine (2µg/rat, intra-hippocampal injection)+iron oxide nanoparticle (7.5mg/kg, IP). Ethics code: EE/96.24.3.88369/SCU.AC.IR Findings: Iron oxide nanoparticles (7.5 mg/kg) increased the anxiety level, compared to the control group (P<0.05). However, pilocarpine injection (1μg/rat) before iron oxide nanoparticles (7.5 mg/kg) improved the anxiety induced by iron oxide nanoparticle (P<0.05). Discussion &Conclusions: It seems that probably the anxiogenic effect of iron oxide nanoparticles is mediated through the reduction of dorsal hippocampal muscarinic cholinergic receptor functions in CA1area.http://sjimu.medilam.ac.ir/article-1-5001-en.htmlAnxietyHippocampusIron oxide nanoparticleMuscarinic receptorsRat |
| spellingShingle | Lotfollah Khajehpour Azam Karimi Mahnaz Kesmati Mozhgan Torabi Involvement of Dorsal Hippocampal Muscarinic Cholinergic Receptors of CA1 Area on Anxiety Induced by Iron Oxide Nanoparticles in Adult Male Rats Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām Anxiety Hippocampus Iron oxide nanoparticle Muscarinic receptors Rat |
| title | Involvement of Dorsal Hippocampal Muscarinic Cholinergic Receptors of CA1 Area on Anxiety Induced by Iron
Oxide Nanoparticles in Adult Male Rats |
| title_full | Involvement of Dorsal Hippocampal Muscarinic Cholinergic Receptors of CA1 Area on Anxiety Induced by Iron
Oxide Nanoparticles in Adult Male Rats |
| title_fullStr | Involvement of Dorsal Hippocampal Muscarinic Cholinergic Receptors of CA1 Area on Anxiety Induced by Iron
Oxide Nanoparticles in Adult Male Rats |
| title_full_unstemmed | Involvement of Dorsal Hippocampal Muscarinic Cholinergic Receptors of CA1 Area on Anxiety Induced by Iron
Oxide Nanoparticles in Adult Male Rats |
| title_short | Involvement of Dorsal Hippocampal Muscarinic Cholinergic Receptors of CA1 Area on Anxiety Induced by Iron
Oxide Nanoparticles in Adult Male Rats |
| title_sort | involvement of dorsal hippocampal muscarinic cholinergic receptors of ca1 area on anxiety induced by iron oxide nanoparticles in adult male rats |
| topic | Anxiety Hippocampus Iron oxide nanoparticle Muscarinic receptors Rat |
| url | http://sjimu.medilam.ac.ir/article-1-5001-en.html |
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